Ensemble structural analyses depict the regulatory mechanism of non-phosphorylated human MAP2K4

Mitogen-activated protein kinase kinase 4 (MAP2K4) plays a critical role in regulating the stress-activated protein kinase signaling cascade. A small angle X-ray scattering experiment, a powerful technique for analyzing a solution structure cleared from the structural artifacts due to crystal packing, provided the ensemble structures of human non-phosphorylated MAP2K4 in three states involving the apo form, the binary complex with an ATP analogue, and the ternary complex with the ATP analogue and substrate peptide. These ensemble structures provided more detailed mechanisms for regulating MAP2K4 in addition to those delineated only by the crystal structures in three states.     

Use of the regression of mean crowding on mean density for estimating sample size and the transformation of data for the analysis of variance

An approximate method for estimating the sample size in simple random sampling and a systematic way of transformation of sample data are derived by using the parameters α and β of the regression of mean crowding on mean density in the spatial distribution per quadrat of animal populations (Iwao , 1968). If the values of α and β have been known for the species concerned, the sample size needed to attain a desired precision can be estimated by simply knowing the approximate level of mean density of the population to be sampled. Also, an appropriate variance stabilizing transformation of sample data can be obtained by the method given here without restrictions on the distribution pattern of the frequency counts.     

A rapid screening method for the isolation of metal-accumulating microorganisms

Summary An agar plate screening method was developed for the rapid isolation of heavy metal-accumulating microorganisms and preliminary estimation of their biosorption capacity. The test is based on the visulaization and interpretation of the metal distribution between agar and colonies by chemical preciptitation with hydrogen sulphide or ammonium sulphide. The heavy metals silver, thallium, lead, copper, nickel and cadmium have been tested successfully. The efficiency of the method is demonstrated for isolating silver-accumulating bacterian and estimating silver biosorption capacity.     

Patella tendon moment arm function considerations for human vastus lateralis force estimates

In vivo muscle forces are typically estimated using literature-based or subject-specific moment arms (MAs) because it is not possible to measure in vivo muscle forces non-invasively. However, even subject-specific muscle-tendon MAs vary across contraction levels and are impossible to determine at high contraction levels without techniques that use ionized radiation. Therefore, different generic MA functions are often used to estimate in vivo muscle forces, which may alter force predictions and the shape of the muscle’s force-length relationship. The aim of this study was to examine the influence of different literature-based patella tendon MA functions on the vastus lateralis (VL) force-angle relationship. Participants (n = 11) performed maximum voluntary isometric knee extension contractions at six knee flexion angles, ranging from 40° to 90°. To estimate in vivo VL muscle force, the peak knee extension torque at each joint angle was multiplied by the VL’s physiological cross-sectional area (PCSA) relative to the quadriceps’ PCSA (34%) and then divided by the angle-specific patella tendon MA for 19 different functions. Maximum VL force was significantly different across MA functions (p ≤ 0.039) and occurred at different knee flexion angles. The shape of the VL force-angle relationship also differed significantly (p < 0.01) across MA functions. According to the maximum force generated by VL based on its literature-derived PSCA, only the VL force-angle relationships estimated using geometric imaging-based MA functions are feasible across the knee angles studied here. We therefore recommend that an average of these MA functions is calculated to estimate quadriceps muscle forces if subject-specific MAs cannot be determined.     

Simulated removal trapping considered in connection with actual census data in small mammals

A record of simulated removal trapping of 50 samples drawn from N=50 under a design has been considered as to how it does contribute to practical censuses of small mammals. It follows that, for ordinary-density populations so far experienced, the removal census based on the theory may frequently lead to quite wrong estimates from single-plot data by the simplest method, and that the maximum likelihood method is doubtless better, but even this method may produce right estimates in about half of the cases, their standard error being of limited availability. Therefore pooling of data from many plots are desirous for such populations.     

Classification of meadow vegetation in the USSR

The scientific classification of meadow vegetation (mesophilous grasslands) was already available in the 1930's due to A. P. Shennikov who suggested to apply new classification principles, on the basis of species dominance. This classification became subjected to criticism, as the criteria proposed did not correspond to the nature of the classification object and as meadow community dominants were instable. In 1971 the Soviet phytocenology was influenced by the ideas of the Braun-Blanquet classification, but before completely adopting it, the Soviet geobotanists employed the so-called dominant-determinant principles of classification, when differential species and dominants were simultaneously used for association determination. In the seventies phytocenoses on the floristic basis, were used though their syntaxonomic analysis was not performed. In 1981, the All-Union conference on vegetation classification was held in Ufa. This conference gave careful consideration to the problem of the floristic classification in the USSR, stated that impressive progress had been achieved in meadow classification on the floristic basis and recommended to use more strict principles for phytocenosis determination alongside with syntaxonomic analysis and subordination of the existing higher syntaxons of the Braun-Blanquet system. The perspectives for implementation of the Braun-Blanquet principles in the USSR are elucidated and a tentative question is raised on the necessity to develop some new classes in order to reflect the specific character of the meadows in the USSR.     

Native peptide mapping – A simple method to routinely monitor higher order structure changes and relation to functional activity

ABSTRACT

In the biopharmaceutical environment, controlling the Critical Quality Attributes (CQA) of a product is essential to prevent changes that affect its safety or efficacy. Physico-chemical techniques and bioassays are used to screen and monitor these CQAs. The higher order structure (HOS) is a CQA that is typically studied using techniques that are not commonly considered amenable to quality control laboratories. Here, we propose a peptide mapping-based method, named native peptide mapping, which could be considered as straightforward for HOS analysis and applicable for IgG4 and IgG1 antibodies. The method was demonstrated to be fit-for-purpose as a stability-indicating assay by showing differences at the peptide level between stressed and unstressed material. The unfolding pathway induced by a heat stress was also studied via native peptide mapping assay. Furthermore, we demonstrated the structure–activity relationship between HOS and biological activity by analyzing different types of stressed samples with a cell-based assay and the native peptide mapping. The correlation between both sets of results was highlighted by monitoring peptides located in the complementary-determining regions and the relative potency of the biotherapeutic product. This relationship represents a useful approach to interrogate the criticality of HOS as a CQA of a drug.