全文获取类型
收费全文 | 145篇 |
免费 | 5篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 2篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 2篇 |
2014年 | 7篇 |
2013年 | 19篇 |
2012年 | 1篇 |
2011年 | 6篇 |
2010年 | 3篇 |
2009年 | 3篇 |
2008年 | 4篇 |
2007年 | 2篇 |
2006年 | 5篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 6篇 |
2002年 | 1篇 |
2001年 | 4篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有150条查询结果,搜索用时 31 毫秒
21.
Rectification of the Olfactory Cyclic Nucleotide-Gated Channel by Intracellular Polyamines 总被引:5,自引:0,他引:5
J.W. Lynch 《The Journal of membrane biology》1999,170(3):213-227
Polyamine-induced inward rectification of cyclic nucleotide-gated channels was studied in inside-out patches from rat olfactory
neurons. The polyamines, spermine, spermidine and putrescine, induced an `instantaneous' voltage-dependent inhibition with
K
d
values at 0 mV of 39, 121 μm and 2.7 mm, respectively. Hill coefficients for inhibition were significantly < 1, suggesting an allosteric inhibitory mechanism. The
Woodhull model for voltage-dependent block predicted that all 3 polyamines bound to a site 1/3 of the electrical distance
through the membrane from the internal side. Instantaneous inhibition was relieved at positive potentials, implying significant
polyamine permeation. Spermine also induced exponential current relaxations to a `steady-state' impermeant level. This inhibition
was also mediated by a binding site 1/3 of the electrical distance through the pore, but with a K
d
of 2.6 mm. Spermine inhibition was explained by postulating two spermine binding sites at a similar depth. Occupation of the first
site occurs rapidly and with high affinity, but once a spermine molecule has bound, it inhibits spermine occupation of the
second binding site via electrostatic repulsion. This repulsion is overcome at higher membrane potentials, but results in
a lower apparent binding affinity for the second spermine molecule. The on-rate constant for the second spermine binding saturated
at a low rate (∼200 sec−1 at +120 mV), providing further evidence for an allosteric mechanism. Polyamine-induced inward rectification was significant
at physiological concentrations.
Received: 17 February 1999/Revised: 27 April 1999 相似文献
22.
Microsympatry in cryptic lowland salamanders (Caudata: Plethodontidae: Bolitoglossa subgenus Nanotriton) from north‐western Honduras: implications for taxonomy and regional biogeography
下载免费PDF全文
![点击此处可从《Journal of Zoological Systematics and Evolutionary Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Alexander J. Hess Michael W. Itgen Thomas J. Firneno James C. Nifong Josiah H. Townsend 《Journal of Zoological Systematics and Evolutionary Research》2017,55(2):150-155
The taxonomy of the diminutive bolitoglossine salamanders of the subgenus Nanotriton has previously been a source of confusion among specialists. At various times, at least three different species of Nanotriton have been reported to occur in Honduras: Bolitoglossa nympha, B. occidentalis and B. rufescens. Phylogenetic analysis of 16S and cyt b from samples from three localities in Honduras (departments of Yoro, Copán and Cortés) confirms that most populations are assignable to B. nympha. Nine samples collected from a single locality on the northern slope of the Sierra de Omoa, between 120 and 190 m in elevation, were found to represent two distinct taxa: B. nympha and B. rufescens. Field examination of these nine specimens found them to be morphologically indistinguishable, including a lack of maxillary teeth in all specimens. As such, in situ identification of the two species in north‐western Honduras is problematic given the occurrence of the two taxa in microsympatry in at least one locality. The discovery of yet another divergent lineage of B. rufescens highlights the need for a taxonomic reassessment within this species complex. 相似文献
23.
Satoshi Inouye Jun-ichi Sato Yuiko Sahara-Miura 《Biochemical and biophysical research communications》2011,(4):792
The mutated recombinant Gaussia luciferase (hgGLase) having the hinge sequence with a reactive cysteine residue at the carboxyl terminal region was purified from Escherichia coli cells by nickel-chelate affinity chromatography and hydrophobic chromatography. The biotinylated hgGLase (Biotin-hgGLase) was prepared by chemical conjugation with a maleimide activated biotin and apply to bioluminescent immunoassay. In the streptavidin and biotin complex system using Biotin-hgGLase, the measurable range of α-fetoprotein as a model analyte was 0.02–100 ng/ml with the coefficient of variation between 2.5% and 5.2%. The sensitivity of Biotin-hgGLase was similar to that by using the detection system of aequorin, alkaline phosophatase and horseradish peroxidase as a label enzyme. 相似文献
24.
25.
A biogeographically mixed, Middle Permian brachiopod fauna from the Baoshan Block, western Yunnan, China 总被引:3,自引:0,他引:3
A small brachiopod fauna is described from the carbonate rocks of the basal Shazipo Formation of the Baoshan Block, western Yunnan, south-west China, including significant new ventral and dorsal internal morphological features of Cryptospirifer omeishanensis Huang. This fauna is regarded as Wordian (Middle Guadalupian, Middle Permian) because of the presence of Cryptospirifer omeishanensis Huang and associated fusulinids ( Neoschwagerina craticulifera Zone). Palaeobiogeographically, the brachiopod fauna is of considerable interest because of its admixed nature characterized by typical warm-water Cathaysian elements intermingled with temperate Peri-Gondwanan taxa. This in turn is interpreted to indicate that the Baoshan Block may have been situated in an intermediate palaeogeographical position between Gondwanaland to the south and Cathaysia to the north during the Mid Permian and, as such, it probably furnished an important 'stepping stone' for the dispersal of Mid Permian eastern Tethyan marine invertebrate taxa (e.g. Cryptospirifer ) to the western Tethys. 相似文献
26.
摘要 目的:研究B超引导下臂丛神经阻滞麻醉对老年桡骨远端粉碎性骨折患者的麻醉效果。方法:选择2018年12月~2020年6月我院的80例老年桡骨远端粉碎性骨折患者,采用随机数字表法,将患者均分为两组。两组均实施臂丛神经阻滞麻醉,其中对照组使用传统的解剖定位法,观察组使用B超引导法。比较两组的麻醉效果、麻醉用药剂量、阻滞起效时间、麻醉完成时间、镇痛维持时间;不同时间的平均动脉压、心率;且记录两组的脊髓麻痹、气胸、呼吸困难、局麻药物中毒发生率。结果:观察组老年桡骨远端粉碎性骨折病人的麻醉效果优良率(95.00 %)明显高于对照组(77.50 %,P<0.05);观察组的麻醉用药剂量、阻滞起效时间、麻醉完成时间均显著低于对照组,镇痛维持时间长于对照组(P<0.05);两组T2和T3时间点的平均动脉压和心率明显高于T1(P<0.05),且观察组的平均动脉压和心率明显更低(P<0.05);观察组的脊髓麻痹、气胸、呼吸困难、局麻药物中毒发生率明显更低(P<0.05)。结论:B超引导臂丛神经阻滞麻醉能提高老年桡骨远端粉碎性骨折患者的麻醉效果。 相似文献
27.
The major advance of mixed liposomes (the so-called chimeric systems) is to control the size, structure, and morphology of these nanoassemblies, and therefore, system colloidal properties, with the aid of a large variety of parameters, such as chemical architecture and composition. The goal of this study is to investigate the alterations of the physicochemical and morphological characteristics of chimeric dipalmitoylphosphatidylcholine (DPPC) liposomes, caused by the incorporation of block and gradient copolymers (different macromolecular architecture) with different chemical compositions (different amounts of hydrophobic component). Light scattering techniques were utilized in order to characterize physicochemically and to delineate the fractal morphology of chimeric liposomes. In this study, we also investigated the structural differences between the prepared chimeric liposomes as are visualized by scanning electron microscopy (SEM). It could be concluded that all the chimeric liposomes have regular structure, as SEM images revealed, while their fractal dimensionality was found to be dependent on the macromolecular architecture of the polymeric guest. 相似文献
28.
We examine how a variety of cationic channels discriminate between ions of differing charge. We construct models of the KcsA potassium channel, voltage gated sodium channel and L-type calcium channel, and show that they all conduct monovalent cations, but that only the calcium channel conducts divalent cations. In the KcsA and sodium channels divalent ions block the channel and prevent any further conduction. We demonstrate that in each case, this discrimination and some of the more complex conductance properties of the channels is a consequence of the electrostatic interaction of the ions with the charges in the channel protein. The KcsA and sodium channels bind divalent ions strongly enough that they cannot be displaced by other ions and thereby block the channel. On the other hand, the calcium channel binds them less strongly such that they can be destabilized by the repulsion of another incoming divalent ion, but not by the lesser repulsion from monovalent ions. 相似文献
29.
30.
The bryozoan fauna from the Xiazhen Formation (Katian, Upper Ordovician) of northeast Jiangxi Province, southeast China is reported here. Seventeen species of bryozoans belonging to fifteen genera and four orders are identified: Homotrypa yushanensis, Homotrypa sp., Prasopora yushanensis, Trematopora sp., Monotrypella sp., Rhombotrypa sp., Orbignyella sp., Constellaria jiangxiensis, Constellaria sp., Stictopora nicholsoni, Trigonodictya parvula, Ptilodictya ensiformis, Stictoporella sp., Pseudopachydictya sp., Nematopora sp., Arthrostylidae sp. indet., and Chasmatoporidae sp. indet. Four of these genera have been reported previously but nine genera (Trematopora, Monotrypella, Rhombotrypa, Orbignyella, Trigonodictya, Ptilodictya, Stictoporella, Pseudopachydictya, and Nematopora), one rhabdomesine and one fenestrate are found for the first time in the Late Ordovician strata of South China. Our palaeogeographical analysis suggests that the bryozoan association is typical for the Katian, which is mostly widespread in Laurentia, Siberia, Baltica and Mediterranean, and displays palaeobiogeographical relationships to the Laurentia–Siberia Province. 相似文献