Impact of transient stress and stress enzymes on development

Stress enzymes triggered by transient stress mediate reprioritization of developmental and homeostatic events to flexibly accomplish the next essential developmental event. This review analyzes recent studies on stress and stress enzyme function during early mammalian development and describes the diverse consequences that result from measurement, analysis of function, and management of stress and stress enzymes during development.     

Biological effects of low-energy C ion implantation on sesame (Sesamum indicum L.)

Field cultivation experiments on white sesame (Sesamum indicum L.)seeds implanted with low-energy C ion showed that different dosages of C ion implantation produce different biological effects.Sesame plants in 6 different dosage groups with C ion density respectively at 1×1011,1×1012,1×1015,5×1015,1×1016,5×1016 ion/cm2 were superior to the control group in plant height,leaf number,stalk diameter and leaf size.Further,sesame plants in these groups flower and seed earlier than those in the control group,and single plant yield also increased.Of all the groups,the 5×1015 ion/cm2 dosage group yielded the best effect,whereas the 1×1017/cm2 dosage group showed an evident inhibitory effect of ion implantation on the germination and growth of the sesame seeds.     

The stimulating effect of recombinant cytokine LIF on the mouse blastocysts during implantation

The effect of recombinant LIF cytokine (Leukemia inhibitory factor) on the isolated mouse embryos at the stages of middle and late blastocyst has been investigated. We have demonstrated here that this agent is necessary in vitro at the stage of normal trophoblast formation after the blastocysts hatch from zona pellucida. This cytokine (10 ng/ml) caused intensification of adhesion and proliferative activity of the trophoblast cells. This is important for intercellular interactions with endometrium and for invasion of embryos into the uterus. The recombinant LIF insignificantly influenced cells of the inner cell mass.     

冠状动脉支架植入患者心理弹性状况调查及与生活质量的关系研究

目的:调查冠状动脉支架植入(ICS)患者心理弹性状况,并分析心理弹性与生活质量的关系。方法:选取2019年3月~2021年4月我院收治的ICS患者120例作为观察组,另选取同期来我院进行健康体检的志愿者80例作为对照组,对比两组患者的生活质量评分,以心理弹性量表(CD-RICS)调查ICS患者的心理弹性状况,采用Pearson相关分析ICS患者生活质量与心理弹性的相关性,采用Logistic回归分析ICS患者心理弹性水平的影响因素。结果:观察组36条目健康调查简表(SF-36)各维度评分及总评分均低于对照组(P<0.05)。120例ICS患者的心理弹性总分为(64.41±5.73)分,心理弹性各维度得分由低到高分别为乐观、力量、坚韧。Pearson相关分析结果表明,ICS患者的生活质量平均分与心理弹性总分呈正相关(P<0.05)。冠心病病程、文化程度、家庭月收入、兴趣爱好数量、居住地对ICS患者的心理弹性得分有影响(P<0.05)。冠心病患病时间≥10年、文化程度为初中及其以下、家庭月收入≤3000元、兴趣爱好为1~2个、居住地为农村是ICS患者心理弹性水平的影响因素(P<0.05)。结论:ICS患者的心理弹性水平一般,且可影响患者生活质量,受到冠心病病程时长、文化程度、家庭收入水平、兴趣爱好、居住地等多种因素影响,应制定合理措施来提高ICS患者的心理弹性,促进患者身心健康。     

听力障碍青少年治疗过程心理干预对患儿语言能力恢复和心境状态的影响

摘要 目的：探讨听力障碍青少年治疗过程心理干预对患儿语言能力恢复和心境状态的影响。方法：选取我院2019年6月到2020年6月共收治的80例7~18岁听力障碍青少年作为研究对象,将患儿随机分为观察组与对照组,每组40例。对所有患儿应用人工耳蜗植入术进行治疗,给予对照组常规言语训练以及健康教育等干预。观察组患儿在常规干预基础上增加全治疗过程的心理干预。对比两组患儿的治疗效果,语言能力恢复,心境状态,ABC评分。结果：观察组患儿治疗的总有效率为92.50%,对照组患儿治疗的总有效率为75.00%,观察组高于对照组（P＜0.05）;两组患儿干预前的言语形成、听觉能力以及听觉感知对比无明显差异（P＞0.05）,两组患儿通过治疗与干预之后,言语形成、听觉能力以及听觉感知明显提升,且观察组评分高于对照组（P＜0.05）;两组患儿干预前的精神、控制、能力、忍受消极情感以及接受变化对比无明显差异（P＞0.05）,两组患儿通过治疗与干预之后,精神、控制、能力、忍受消极情感以及接受变化明显降低,且观察组评分低于对照组（P＜0.05）;两组干预前的兴奋、易激怒、刻板行为以及不恰当语言等评分比较无统计学意义（P＞0.05）,两组患儿干预后,各评分均下降,其中观察组小于对照组,差异有统计学意义（P＜0.05）。结论：对听力障碍青少年在常规治疗与干预基础上增加心理干预,能够提升患儿的治疗效果,提升语言能力恢复情况,减少负面情绪,提升患儿的心境状态,值得临床应用推广。     

Calcitonin administration improves endometrial receptivity via regulation of LIF,Muc-1 and microRNA Let-7a in mice

Calcitonin (CT) is one of the factors affecting the embryo implantation, but its effects on the implantation window have not been fully investigated. The current study investigated the effects of CT on the endometrium receptivity by morphological study and evaluation of leukemia inhibitory factor (LIF), mucin 1 (Muc-1), and microRNA (miRNA) Let-7a in the ovarian stimulation and the normal ovarian cycle. Then the mechanism of the CT effects through the mammalian target of rapamycin (mTOR) signaling pathway was studied by using PP242. A total of 64 BALB/c mice were divided into the normal ovarian cycle and ovarian stimulation groups. Each group consisted of four subgroups: control, calcitonin, PP242, and calcitonin+PP242. CT and PP242 were injected on the fourth of pregnancy into the mice and 24 hr later all the mice were killed. The uterine tissue samples were used for morphological analysis, and endometrial cells were mechanically isolated for evaluation of gene and protein expression. The results showed that ovarian stimulation induced mTOR phosphorylation as well as increased expression of the Let-7a miRNA. In addition, CT injection increased the expression of LIF and miRNA Let-7a in ovarian stimulation similar to that in normal ovarian cycles. However, injection of PP242 reduced expression of miRNA Let-7a and increased Muc-1 expression in ovarian stimulation group. In conclusion, the administration of CT improved endometrial receptivity in mice. This phenomenon occurred by upregulation of LIF, miRNA Let-7a and downregulation of Muc-1 via mTOR signaling pathway.     

First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free sperm vitrification protocol

PurposeThe purpose of this study is to present the first birth of healthy infant born following ICSI using the new permeable cryoprotectant-free sperm vitrification protocol Easy-Sperm^®.Principal resultsA 39 years old woman and his 40 years old partner underwent egg donation treatment at IVF-Spain Alicante (Spain). Half of the mature oocytes obtained from a young and healthy donor were fertilized by ICSI, using slow-frozen spermatozoa and the other half with vitrified spermatozoa. A total of 5 blastocysts were obtained on day 5 (3 resulting from vitrified spermatozoa and 2 from frozen sperm). The best embryo, with AA quality (derived from one of the oocytes fertilized with vitrified sperm) was transferred. The woman conceived and, following a normal pregnancy, delivered a healthy boy.ConclusionsTo the best of our knowledge, this is the first case report of a successful pregnancy and delivery of a healthy infant from ICSI with permeable vitrified spermatozoa in an oocyte donation program with transfer on blastocyst stage.     

Transcriptome analysis of the endometrium from Chinese Erhualian sows that differ in calcium ion concentration and litter size

Embryonic survival rate, an important factor in the fecundity of sows, is affected by endometrium‐secreting histotroph. A higher concentration of calcium ion has been observed in the uterus of highly prolific Erhualian sows (EH) compared with those of less prolific (EL) sows. This suggests that EH sows have better establishment and maintenance of pregnancies, thus increasing embryonic survival rate during the peri‐implantation period. To understand the mechanisms of how the endometrium‐secreting histotroph affects embryonic survival rate during the Erhualian peri‐implantation period, the expression patterns of endometrial mRNA in the EH and EL sows on day 12 of gestation were analyzed using RNA sequencing technology. A total of 164 differentially expressed genes (DEGs) were identified (P_adj < 0.05, |log₂(FC)| ≥ 1), including 46 upregulated and 118 downregulated genes in EH compared to EL. Gene Ontology enrichment indicated that a subset of DEGs was involved in calcium ion binding and cell adhesion. Solute carrier family 8 member A3 and solute carrier family 24 member 4, identified as upregulated genes (P_adj < 0.05) in EH, were considered key candidate genes expressed in the endometrium affecting embryonic survival rate during the peri‐implantation period. The results improve understanding of the genetic mechanism underlying the variation in litter size of Erhualian pigs during the peri‐implantation period.     

离子注入后诱导水稻多倍化的效果

利用3份二倍体水稻为材料,以氮离子束为诱变源,研究了离子注入后所引起的生物学效应和离子注入对水稻多倍化的效果。研究结果表明,氮离子束对3份二倍体水稻材料的效应因材料种类和离子注入剂量不同而异。N 注入处理后对利用秋水仙素诱导水稻多倍化的效果比较明显,但效果的明显程度则因离子注入剂量不同或秋水仙素诱导时间不同或试验材料的遗传背景不同而表现出一定的差异。在秋水仙素对试验材料的诱导时间为24 h的各个处理中,N 离子注入剂量为6.76×1016N /cm2的处理似乎更好,而在秋水仙素对试验材料的诱导时间为48 h的各个处理中,N 离子注入剂量为0.52×1016N /cm2的处理则更有利于获得同源四倍体材料。经过2个~3个世代的筛选之后并通过染色体核型分析,在试验材料的后代中已经获得了一些同源四倍体水稻材料。