Accumulation of inositol phosphates and cyclic AMP in guinea-pig cerebral cortical preparations: Effects of norepinephrine,histamine, carbamylcholine and 2-chloroadenosine

Norepinephrine and serotonin augment by about 2-fold the accumulation of cyclic [3H]AMP elicited by 2-chloroadenosine in [³H]adenine-labeled guinea-pig cerebral cortical slices. Histamine causes a 3-fold augmentation. The first two agents have no effect on cyclic AMP alone, while histamine has only a small effect alone. The augmentation of the 2-chloroadenosine response appears to be mediated by α₁-adrenergic, 5HT₂-serotonergic and H₂-histaminergic receptors. VIP-elicited accumulations of cyclic AMP are also augmented through stimulation of α₁-adrenergic, 5HT₂-serotonergic and H₁-histaminergic receptors. Activation of these amine receptors also increases the turnover of phosphatidylinositols in [³H]inositol-labeled guinea pig cerebral cortical slices. Norepinephrine causes a 5-fold, serotonin a 1.2-fold, and histamine a 2.5-fold increase in accumulations of [³H]inositol phosphates. 2-Chloroadenosine, vasoactive intestinal peptide, baclofen, and somatostatin have no effect on phosphatidylinositol turnover, nor do the last two agents augment accumulations of cyclic AMP elicited by 2-chloroadenosine. The data suggest a possible relationship between turnover of phosphatidylinositol and the augmentations of the cyclic AMP accumulations elicited by biogenic amines in brain slices.     

Biogene Amine im Gehirn vom Frosch (Rana esculenta)

Zusammenfassung Mit Hilfe der Methode zur fluoreszenzmikroskopischen Lokalisation von Catechol- und Tryptaminen wurde die Verteilung dieser Stoffe im ZNS von Rana esculenta untersucht. Catecholamin- und serotoninhaltige Neurone liegen im Nucleus reticularis mesencephali. Außerdem finden sich catecholaminhaltige Nervenzellen im Organon vasculosum hypothalami und in der Area praeoptica. Diese aminproduzierenden Zellen entsenden Zellfortsätze durch die Ependymschicht in den Ventrikel. Über diese Ausläufer erfolgt möglicherweise eine Sekretion biogener Amine in den Liquor cerebrospinalis. Catecholamin- und serotoninhaltige Axone erreichen voneinander verschiedene Kerngebiete und Areale. Neben dem periventrikulären Zellager im Tuber cinereum und in der Area praeoptica werden vor allem der ventrolaterale Teil des lateralen Septumkerns, Striatum ventrale und Epistriatum von Endstrecken catecholaminhaltiger Axone durchdrungen. Serotoninhaltige Varicositäten finden sich dagegen vor allem in Kerngebieten, die in sensorische Bahnen eingeschaltet sind (Nucleus isthmi, corpus geniculatum laterale, Area praetectalis, Tectum opticum, Thalamus dorsalis, Neostriatum). Weitere Ausbreitungsgebiete 5-Hydroxytryptamin-haltiger Fasern sind die Habenula und der Nucleus interpeduncularis, Kerngebiete, über die Erregungen aus dem limbischen System auf vegetative Zentren der Medulla oblongata geleitet werden.

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Biogenic amines in the brain of the frog (Rana esculenta)

Summary The distribution of biogenic amines in the central nervous system of Rana esculenta was investigated by means of the fluorescence-microscopical detection of catecholand tryptamines. The nucleus reticularis mesencephali was found to contain numerous neurones rich in catechol- and tryptamines. Apart from this nucleus nerve cells in the organon vasculosum hypothalami and in the area praeoptica were found to contain catecholamines. The clublike processes of these neurones penetrate the ependymal layer and extend into the ventricle. These structures are presumably responsible for a secretion of biogenic amines into the cerebrospinal fluid. Catecholamine- and serotonin-containing axons terminate on different nuclei and areas. Besides the periventricular cellular layer of the tuber cinereum and the area praeoptica, the pars ventrolateralis of the nucleus septalis lateralis, striatum ventrale and epistriatum are pervaded by terminals of catecholamine-containing neurons. Serotonincontaining varicosities are mainly to be found in nuclei, which are intercalated in sensory pathways (nucleus isthmi, corpus geniculatum laterale, area praetectalis, tectum opticum, thalamus dorsalis, neostriatum). Further areas of distribution of 5-hydroxytryptamine-fibers are the habenula and the nucleus interpeduncularis, nuclei which coordinate impulses from the limbic system projecting them on visceral centers of the medulla oblongata.

Mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft.     

Isoprene emissions and photosynthesis in three ferns – The influence of light and temperature

A study was designed to determine the rates of isoprene emission and photosynthesis in three fern species [ Dicksonia antarctica Labill., Thelypteris decursive-pinnata (Van Hall) Ching and Thelypteris kunthii (Desv.) Morton] and the independent influence of light and temperature on these processes. The plants were conditioned in a growth chamber and then transferred to a controlled environment gas-exchange chamber. Samples of the chamber atmosphere were collected; isoprene was concentrated cryo-genically and measured by gas chromatography. Only small amounts of isoprene were detected around the ferns in the dark. Isoprene emissions increased with increasing levels of photosynthetic photon flux density (PPFD) in all three species; 50% of the maximum emission occurred at PPFD levels of 130 to 500 μmol m−² s−¹. Maximum isoprene emissions occurred between 35 and 39°C which is a lower temperature maximum than reported for angiosperms and gymnosperms. The increased emissions with temperature were primarily associated with increased biosynthetic rates for isoprene. Carbon lost through isoprene accounted for 0.02 to 2.6% of the carbon fixed during photosynthesis, depending on the PPFD level, temperature and fern species.     

Analysis of Biogenic Amines in Bovine Retina by Gas Chromatography-Negative Ion Chemical Ionisation Mass Spectrometry

Biogenic amines in bovine retina have been identified and quantified by an extraction-derivatisation procedure involving their reaction with 3,5-di(trifluoromethyl)benzoyl chloride (DTFMBCl) in the aqueous phase followed by extraction into an organic solvent, hydrolysis of phenolic esters, and conversion of free hydroxyl groups to trimethylsilyl ethers. Subsequent analysis of these DTFMB-trimethylsilyl derivatives by gas chromatography-negative ion chemical ionisation mass spectrometry revealed that the molecular ion carried most (greater than 60%) of the ion current, which made the method highly specific and gave a potential limit of detection below the picogram level. This method establishes unequivocally that the principal amines in bovine retina are p-tyramine, dopamine, and 5-hydroxytryptamine.     

Physiological characterization of the cold-shock-induced humoral factor for wing color-pattern changes in butterflies

Butterfly wing color patterns can be modified by the application of temperature shock to pupae immediately after pupation, which has been attributed to a cold-shock-induced humoral factor called cold-shock hormone (CSH). Here, we physiologically characterized CSH and pharmacological action of tungstate, using a nymphalid butterfly Junonia orithya. We first showed that the precise patterns of modification were dependent on the time-point of the cold-shock treatment after pupation, and confirmed that the modification properties induced in a cold-shocked pupa were able to be transferred to another pupa in a parabiosis experiment. Cold-shock application after removal of the head and prothorax together still produced modified wings, excluding major involvement of the brain-retrocerebral neuroendocrine complex. Furthermore, tungstate injection induced modifications even in individuals whose head and prothorax were removed. Importantly, transplantation of tracheae isolated from cold-shocked pupae induced modifications in the recipient wings. We identified a chemical peak in hemolymph of the cold-shocked individuals using HPLC, which corresponded to dopamine, and demonstrated that dopamine and its related biogenic amines have ability to induce small color-pattern changes. Taken together, the present study suggests that CSH is likely to be secreted from trachea-associated endocrine cells upon cold-shock treatment and that tungstate may change color patterns via its direct action on wings.     

Mikrospektrofluorometrische Untersuchungen der biogenen Amine im Glomus caroticum des Kaninchens nach Reserpin- und PCPA-Applikation

Zusammenfassung Die Glomera carotica von 12 Kaninchen wurden nach viertägiger Applikation von Reserpin (2 mg/kg KG; i.p.) (I), Parachlorphenylalanin-methylester-hydrochlorid (PCPA; 100 mg/kg KG; i.p.) (II) und physiologischer NaCl-Lösung (III) mit der Falckschen Fluoreszenzmethode aufgearbeitet und cytospektrofluorometrisch (Mikrospektrograph Leitz) ausgewertet.An Hand der Fluoreszenzspektren der spezifischen Glomuszellen (Typ I) ließ sich eine Depletion der Catecholamine und des Serotonins verfolgen: hiernach senkt PCPA vor allem das Serotonin, Reserpin hingegen die Catechol- und Indolamine in etwa gleichem Umfang.Durch Zerlegung einiger Gesamtspektren in die Anteile für Catecholamine und das Serotonin mit Hilfe eines Iterationsverfahrens der nichtlinearen Regression konnte gezeigt werden, daß nur eine geringfügige Beeinflussung der Catecholamin-Peakhöhe durch das Serotonin vorliegt. Über die Messung der Peakhöhe im Catecholaminbereich der Gesamtspektren konnte daher die Reduktion der Catecholamine auf 64±22% durch PCPA und auf 13±4% durch Reserpin statistisch gesichert werden.Die Befunde werden im Zusammenhang mit den möglichen Wirkungsmechanismen der applizierten Substanzen diskutiert. Eine ausführliche tabellarische Zusammenstellung der bisherigen Angaben über Gehalt und Zusammensetzung der biogenen Amine des Glomus caroticum ergänzt diese Diskussion.

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Microspectrofluorometric investigation of biogenic amines in the rabbit glomus caroticum after reserpine and PCPA treatment

Summary After the administration for 4 days of (I) reserpine (2 mg/kg body weight daily, i.p.), (II) p-chlorophenylalanine-methylester-hydrochloride (PCPA; 100 mg/kg body weight daily; i.p.) and III physiological NaCl solution, the carotid bodies of 12 rabbits were examined using Falck's fluorescence technique and evaluated cytospectrofluorometrically. Fluorescence spectra of the type I specific glomus cells revealed that a depletion of catecholamines and serotonin took place: PCPA lowered mainly serotonin, whereas reserpine depleted catechol- and indolamines about equally.It was shown by splitting of a few combined spectra with an iteration procedure of nonlinear regression, that serotonin affected slightly the height of the catecholamine peak. Thus, measuring the peak height in the catecholamine region of the total spectrum, the reduction of catecholamines to 64±22% by PCPA and to 13±4% by reserpine was statistically significant.These data are discussed in relation to possible mechanisms of action of the test substances and related to the content and nature of the biogenic amines in the carotid body.

Für technische Hilfe danken wir Fräulein B. Remki und Frau K. Wilp.     

Potential influence of tubicolous worms on the bottom roughness length z0 in the south-western Baltic Sea

The influence of three different species of tubicolous worms (Pygospio elegans, Polydora ciliata and Lagis koreni) on the hydrodynamic bottom roughness length (z₀) was analysed in this study. Flume experiments and geospatial methods were combined to determine the potential interactions between worm tubes and the near-bed flow regime and the resulting effects on sediment transport in the south-western Baltic Sea. The three selected species are common in the area of interest (3539 km²). Their species-specific population densities were taken from existing macrozoobenthos datasets and transferred into a Geographic Information System (GIS). In analogy to the sediment roughness length, the hydrodynamic roughness lengths generated by the tubicolous worms were calculated and corresponding sediment transport values, derived from flume experiments with artificial tube lawns, were geospatially analysed using GIS. In order to show the direct influence of worm tubes on the surrounding sediment surface flume experiments were conducted at two given current velocities of 20 cm s^− 1 for sediment displacement effects and 5 cm s^− 1 for deposition effects. The roughness length was shown to increase by a factor of 2 to 30 in the presence of biogenic structures such as the worm tubes. The near-bed hydrodynamic conditions are significantly influenced at low roughness densities through independent or isolated flow conditions at 0.7 to 1.9% and at high roughness densities between 4.2 and 7.5%, resulting in unaffected sediment surfaces through “skimming flow”, as well. The GIS analysis revealed that this effect occurs over 4% (137 km²) of the area of investigation, whereas sediment displacement at roughness densities between 0.7 and 1.9% due to increased turbulence is the predominant effect over 33% (present on 1172 km²) of the area of investigation. These findings reveal the important influence of species-generated microtopography on sediment transport processes.     

Influence of the mode of macrofauna-mediated bioturbation on the vertical distribution of living benthic foraminifera: First insight from axial tomodensitometry

We investigated the influence of bioturbation by macrofauna on the vertical distribution of living (stained) benthic foraminifera in marine intertidal sediments. We investigated the links between macrofaunal bioturbation and foraminiferal distribution, by sampling from stations situated on a gradient of perturbation by oyster-farming, which has a major effect on benthic faunal assemblages. Sediment cores were collected on the French Atlantic coast, from three intertidal stations: an oyster farm, an area without oysters but affected by oyster biodeposits, and a control station. Axial tomodensitometry (CT-scan) was used for three-dimensional visualization and two-dimensional analysis of the cores. Biogenic structure volumes were quantified and compared between cores. We collected the macrofauna, living foraminifera, shells and gravel from the cores after scanning, to validate image analysis. We did not investigate differences in the biogenic structure volume between cores. However, biogenic structure volume is not necessarily proportional to the extent of bioturbation in a core, given that many biodiffusive activities cannot be detected on CT-scans. Biodiffusors and larger gallery-diffusors were abundant in macrofaunal assemblage at the control station. By contrast, macrofaunal assemblages consisted principally of downward-conveyors at the two stations affected by oyster farming. At the control station, the vertical distribution of biogenic structures mainly built by the biodiffusor Scorbicularia plana and the large gallery-diffusor Hediste diversicolor was significantly correlated with the vertical profiles of living foraminifera in the sediment, whereas vertical distributions of foraminifera and downward-conveyors were not correlated at the station affected by oyster farming. This relationship was probably responsible for the collection of foraminifera in deep sediment layers (> 6 cm below the sediment surface) at the control station. As previously suggested for other species, oxygen diffusion may occur via the burrows built by S. plana and H. diversicolor, potentially increasing oxygen penetration and providing a favorable microhabitat for foraminifera in terms of oxygen levels. By contrast, the absence of living foraminifera below 6 cm at the stations affected by oyster farming was probably associated with a lack of biodiffusor and large gallery-diffusor bioturbation. Our findings suggest that the effect of macrofaunal bioturbation on the vertical distribution of foraminiferal assemblages in sediments depends on the effects of the macrofauna on bioirrigation and sediment oxidation, as deduced by Eh values, rather than on the biogenic structure volume produced by macrofauna. The loss of bioturbator functional diversity due to oyster farming may thus indirectly affect infaunal communities by suppressing favorable microhabitats produced by bioturbation.     

昆虫体内章鱼胺和酪胺的研究进展

章鱼胺(octopamine, OA)和酪胺(tyramine, TA)在昆虫体内扮演着各种重要的生理角色。它们协调控制着昆虫的各种器官和行为, 如调节外周淋巴器官功能和影响昆虫的学习与记忆、昼夜节律等, 使得昆虫能够以合理的方式来应对外界刺激, 并被认为在功能上对应于脊椎动物体内的肾上腺素和去甲肾上腺素。虽然都是酪氨酸脱羧基产物, 且酪胺是章鱼胺的生物合成前体, 但它们都通过不同的G蛋白偶联受体在昆虫体内发挥不同的神经调控作用。近年来, 对昆虫体内章鱼胺和酪胺, 尤其是它们与对应受体作用的研究, 日益受到关注。本文对昆虫体内章鱼胺和酪胺的生物合成, 在神经和非神经组织中的分布, 被突触前结构的再摄取以及它们在昆虫体内的不同生理功能等方面的研究进展进行了综述, 特别对章鱼胺和酪胺受体基因的克隆、信号转导途径以及药理作用特性等相关研究的最新进展进行了详细评述。     

Histamine signalling in Schistosoma mansoni: Immunolocalisation and characterisation of a new histamine-responsive receptor (SmGPR-2)

In parasitic platyhelminthes, including Schistosoma mansoni, biogenic amines play several important roles in the control of motility, metabolism and reproduction. A bioinformatics analysis of the S. mansoni genome identified approximately 16 full-length G protein-coupled receptors (GPCRs) that share significant homology with aminergic receptors from other species. Six of these sequences are structurally related to SmGPR-1 (formerly SmGPCR), a previously described histamine receptor of S. mansoni, and constitute a new clade of amine-like GPCRs. Here we report the cloning of a second member of this clade, named SmGPR-2. The full-length receptor cDNA was expressed in Saccharomyces cerevisiae and shown to be activated by histamine and 1-methylhistamine, whereas other common biogenic amines had no significant effect. Antagonist assays showed that SmGPR-2 was inhibited by classical biogenic amine antagonists but the pharmacological profile was unlike those of known mammalian histamine receptors. Confocal immunolocalisation studies revealed that SmGPR-2 was expressed in the nervous system and was particularly enriched in the subtegumental neuronal plexus of adult S. mansoni and larvae. The ligand, histamine, was found to be widely distributed, mainly in the peripheral nervous system including the subtegumental plexus where the receptor is also expressed. Finally, SmGPR-2 was shown to be developmentally regulated at the RNA level. Quantitative PCR studies showed it was up-regulated in the parasitic stages compared with cercaria and expressed at the highest level in young schistosomula. The widespread distribution of histamine and the presence of at least two receptors in S. mansoni suggest that this transmitter is an important neuroactive substance in schistosomes.