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151.
Having developed a method to contaminate the breeding sites of the cockchafer with the aid of infected females, two large field trials were carried out, one in 1985 and the other in 1988. Blastospores were applied by helicopter at 26 sites of woodland borders, where the swarming beetles aggregate. The mean dose amounted to 2.0-3.7 × 1014 spores ha-1. The infection rates of the treated adults ranged from 30 to 99%. The treatment reduced the average reproduction rate from 5.09 to 2.15 second instar larvae/adult. The development of the treated populations was followed at 15 sites in perennial grassland by examining samples taken in the winter season. Increased infection rates were observed in the generation following the treatment. The population density 6 and 9 years after the treatment, corresponding to two and three generations of the beetle respectively, decreased at 13 sites by more than 50% and at four sites by more than 80%. The population densities remained high at only two sites, although the fungus was present. During this period, the area of heavy feeding damage by the adults at woodland borders decreased from 17.8 to 3.9 km. At the same time, damage increased outside the treated area from 27 to 31 km. The advantages and disadvantages of this method of breeding site contamination are discussed. 相似文献
152.
This paper presents the results of an exploratory study to determine whether vesicular-arbuscular mycorrhizal (VAM) fungi affect the success of insects used as weed biological agents. No direct correlation between the colonization by VAM fungi alone and the success of root- and shoot-feeding insects was found. However, in Centaurea diffusa , plants colonized by VAM fungi had a lower shoot root ratio and increased attack and survival of the root beetle, Sphenoptera jugoslavica , in the field. Beetle success was also best on C. diffusa when the plant was growing among determinate grasses that largely cease growing after flowering in the spring or early summer. This is consistent with other reports that VAM hyphae can transfer nutrients between plant species from low to high sinks. From these results and related reports in the literature, it is suggested that plants with many stenophagous root-insects are those in which VAM fungi colonization increases nutrient partitioning to the roots with little or no decrease in plant growth. Root-feeding, rather than foliage- or seed-feeding, insects seem to be particularly effective as biocontrol agents for plants of this type. 相似文献
153.
D. J. Lactin P. Harris D. L. Johnson F. -H. Wan A. G. Thomas 《Biocontrol Science and Technology》1997,7(4):657-670
Leaf - feeding beetles , Altica carduorum, from a population in NW China have been identified as candidate agents for biocontrol of Canada thistle , Cirsium arvense. This paper assesses the potential of beetles from this population to establish on the Canadian prairies . A phenological model is applied to determine where sufficient heat accumulates to allow completion of at least one generation per year . The model was applied both with and without a submodel of adult thermoregulation . The model was driven using meteorological data from a grid covering the agricultural region of the Canadian prairies , i . e . ca . 670 000 km 2 , at a resolution of (50 50) km . In each grid square , the percentage of the years 1960 - 89 for which the model indicated that at least one generation could have been completed was calculated; these proportions were categorized (0 - 80%; 80 - 99% and 100%) and mapped . Maps of C. arvense density over the simulation region were also produced , and compared to the maps of predicted beetle distribution . The model suggests that A. carduorum could establish over much of the prairies . Without thermoregulation , the predicted range (i . e . the area in which one generation was completed in every year) is restricted to the warmer parts of the prairies , where C. arvense is generally sparse . Inclusion of thermoregulation almost doubled the predicted range to cover most of the range of C. arvense on the Canadian prairies , missing only the cooler peripheral areas . These results suggest that this strain of A. carduorum has potential to overlap the range of C. arvense on the Canadian prairies , and so has potential to control this weed . 相似文献
154.
DARRYL W M COOK PETER G LONG SIVA GANESH L-H CHEAH 《The Annals of applied biology》1997,131(3):503-518
Bacteria and yeasts, selected by an attachment assay for their ability to adhere to Botrytis cinerea hyphae or conidia, were evaluated for biocontrol potential against B. cinerea on excised tomato stems. Eight of the 12 bacteria and seven of the eight yeast isolates conferred 90% to 100% biocontrol activity when antagonist populations were applied at three to 80 times the pathogen inoculum density. Biocontrol was maintained at similar levels when biocontrol agent (BCA) application was delayed up to 48 h after pathogen challenge. Scanning electron microscopy showed extensive colonisation of B. cinerea mycelium or conidia by many of these isolates and also evidence of pathogen degradation. The biocontrol efficacy and potential for the assay are discussed with respect to cell-to-cell adhesion as a vehicle to deliver antagonistic mechanisms to highly specific pathogen sites. 相似文献
155.
1 The pattern of dispersion within plants of the two-spotted spider mite, Tetranychus urticae, and its predator, the phytoseiid Phytoseiulus persimilis, was studied on the dwarf hop variety First Gold from May to September in 1997 and 1998. 2 Spider mite populations developed on the lower leaves initially but, by late July, as the numbers of mites increased, most were found towards the top of plants. From early August, the numbers of spider mites decreased most rapidly on the upper parts of plants. 3 Where P. persimilis was released, the predator maintained the numbers of T. urticae below those found on non-release plots throughout the season. 4 By early August, the predator’s pattern of dispersion was similar to that of the pest. 5 Predators spread to non-release plots by 20 June in 1997 and 24 July in 1998 and eventually became more numerous than on the plots where they had been released. 相似文献
156.
Effects of water extracts of a composted manure-straw mixture on the plant pathogen Botrytis cinerea 总被引:1,自引:0,他引:1
M. P. McQuilken J. M. Whipps J. M. Lynch 《World journal of microbiology & biotechnology》1994,10(1):20-26
Manure-straw mixtures were composted and water extracts, made by incubating compost in water for 3 to 18 days, were assessed for antagonistic activity against Botrytis cinerea, using a range of tests. Extracts of all ages inhibited conidial germination on glass slides and reduced mycelial growth on agar. Mixing extracts of all ages with droplets of suspensions of B. cinerea conidia on detached Phaseolus bean leaves suppressed lesion development, but only 3- to 8-day-old extracts had an effect when sprayed onto leaves 2 days before inoculation. Extracts contained a large and varied microbial population of actinomycetes (0.3 to 2.4×105 c.f.u.ml–1), bacteria (1.5 to 5.6×1010 c.f.u.ml–1), filamentous fungi (25.0 to 45.5 c.f.u. ml–1) and yeasts (26.1 to 62.6 c.f.u.ml–1). Eight- and 18-day-old extracts lost activity completely on filter sterilization or autoclaving. Weekly sprays of 8-day-old extracts onto lettuce in the glasshouse had no effect on the incidence of grey mould, but significantly reduced its severity and increased marketable yield. The use of compost extracts in biocontrol of plant diseases and their possible mode of action is discussed.M.P. McQuilken and J.M. Whipps are and J.M. Lynch was with the Microbiology and Crop Protection Department, Horticulture Research International, Littlehampton BN17 6LP, UK; J.M. Lynch is now with the School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK. 相似文献
157.
Yue Kong Rong Wang Da-Rong Yang Rachakonda Sreekar Stephen G. Compton 《Biocontrol Science and Technology》2016,26(10):1432-1443
Classical biological control agents fail to achieve an impact on their hosts for a variety of reasons and an understanding of why they fail can help shape decisions on subsequent releases. Ornamental Ficus microcarpa is a widely planted avenue fig tree that is invasive in countries where its pollinator (Eupristina verticillata) is also introduced. This tree also supports more than 20 species of non-pollinating fig wasps (NPFW) that feed in the figs and have the potential to reduce the plant’s reproduction. Odontofroggatia galili, one of the most widely introduced NPFW, has larvae that develop in galled ovules that might otherwise develop into seeds or support pollinator larvae. We examined the distribution and relative abundance of the pollinator and O. galili on F. microcarpa in China, towards the northern limit of the tree’s natural range, and in Italy where the two species have been introduced. Where they co-existed, we also recorded the impact of varying densities of O. galili on F. microcarpa seed and pollinator production. O. galili and E. verticillata displayed contrasting habitat preferences in China, with O. galili almost absent from warmer sites. O. galili abundance and sex ratios varied between the natural and introduced ranges. Figs with more O. galili contained fewer seeds and pollinator offspring, but reproduction was rarely inhibited totally. Additional species with a greater impact in the figs they occupy are needed if biocontrol of F. microcarpa is to be effective. 相似文献
158.
Aydi Ben Abdallah Rania Hayfa Jabnoun-Khiareddine Ahlem Nefzi Sonia Mokni-Tlili Mejda Daami-Remadi 《Biocontrol Science and Technology》2016,26(8):1139-1165
Nine non-pathogenic bacterial isolates, recovered from Datura metel organs and able to colonise the internal stem tissues of tomato cultivar Rio Grande, were screened for their ability to suppress tomato Fusarium wilt disease caused by Fusarium oxysporum f. sp. lycopersici (FOL), and to enhance plant growth. S33 and S85 isolates tested were found to be the most effective in decreasing Fusarium wilt severity by 94–95% compared to FOL-inoculated and untreated control. A significant enhancement of growth parameters was recorded on tomato plants inoculated or not with FOL. Both isolates were characterised and identified using 16S rDNA sequencing genes as Stenotrophomonas sp. str. S33 (KR818084) and Pseudomonas sp. str. S85 (KR818087). Screened in vitro for their antifungal activity towards FOL, these isolates led to 38.7% and 22.5% decrease in pathogen radial growth and to the formation of an inhibition zone of 12.75 and 8.37?mm respectively. Stenotrophomonas sp. str. S33 and Pseudomonas sp. str. S85 were found to be chitinase-, protease- and pectinase-producing strains but unable to produce hydrogen cyanide. Production of indole-3-acetic acid-like compounds, phosphate solubilising ability and pectinase activity were investigated for elucidating their plant growth-promoting traits and their endophytic colonisation ability. 相似文献
159.
《Fungal biology》2020,124(1):54-64
The role of the sesquiterpene botrydial in the interaction of the phytopathogenic fungus Botrytis cinerea and plant-associated bacteria was analyzed. From a collection of soil and phyllospheric bacteria, nine strains sensitive to growth-inhibition by B. cinerea were identified. B. cinerea mutants unable to produce botrydial caused no bacterial inhibition, thus demonstrating the inhibitory role of botrydial. A taxonomic analysis showed that these bacteria corresponded to different Bacillus species (six strains), Pseudomonas yamanorum (two strains) and Erwinia aphidicola (one strain). Inoculation of WT and botrydial non-producing mutants of B. cinerea along with Bacillus amyloliquefaciens strain MEP218 in soil demonstrated that both microorganisms exert reciprocal inhibitory effects; the inhibition caused by B. cinerea being dependent on botrydial production. Moreover, botrydial production was modulated by the presence of B. amyloliquefaciens MEP218 in confrontation assays in vitro. Purified botrydial in turn, inhibited growth of Bacillus strains in vitro and cyclic lipopeptide (surfactin) production by B. amyloliquefaciens MEP218. As a whole, results demonstrate that botrydial confers B. cinerea the ability to inhibit potential biocontrol bacteria of the genus Bacillus. We propose that resistance to botrydial could be used as an additional criterion for the selection of biocontrol agents of plant diseases caused by B. cinerea. 相似文献
160.
多功能菌群混合施用的生态效应 总被引:7,自引:0,他引:7
从蔬菜地黄瓜根围土壤中经分离筛选,得到两个对黄瓜枯萎病菌(Fusarium oxysporum f.sp.cucumerinum)有抑制作用,并能明显促进黄瓜生长的菌株ZJY-1(Brevibacillus brevis)和ZJY-116(Bacillus subtilis).将这两菌株与一株毒死蜱降解菌DSP3(Alcaligenes faecali)按照不同组合处理黄瓜种子,以研究多功能菌群在环境中发挥生态效应的可行性.结果表明,这两株具有生防和促生功能的菌株与DSP3按不同组合混合后,对黄瓜的防病促生作用均不受影响.同时对黄瓜根围土中毒死蜱含量检测分析发现,DSP3与这两株细菌混和施用后其降解率与单独施用相比没有明显差异.实验初步证明,不同功能的菌株混合施用后可以发挥其生态效应. 相似文献