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951.
Ferdinand Bohlmann Christa Zdero Robert M. King Harold Robinson 《Phytochemistry》1982,21(8):2021-2025
The investigation of Stevia myriadenia afforded in addition to known compounds a new clerodane and a labdane derivative, two guaianolides, a bisabolene and a germacrene derivative. The structures were elucidated by spectroscopic methods. The chemotaxonomic situation is discussed briefly. 相似文献
952.
David C. Fork 《Photosynthesis research》1996,49(1):91-101
Charles Stacy French, one of the great men of photosynthesis research, died on 13 October 1995. He received his PhD at Harvard University where he associated with William Arnold, Caryl Haskins, later president of the Carnegie Institution of Washington, and Pei-Sung Tang. He did early work on the photosynthesis of photosynthetic bacteria with Robert Emerson and later with Otto Warburg. French worked for three years with James Franck in Chicago. His associates there included Hans Gaffron, Robert Livingston, Warren Butler and Roderick Clayton. After spending three years at the University of Minnesota he became the director of the Department of Plant Biology of the Carnegie Institution of Washington and remained there until he retired in 1973. French's research career at the Carnegie Institution was marked by the development of novel and ingenious pieces of equipment such as the French pressure cell used to prepare chloroplast particles to measure partial reactions of photosynthesis. He developed the first recording fluorescence spectrophotometer and demonstrated efficient energy transfer between certain photosynthetically-active pigments, a spectrophotometer that measured the first derivative of absorbance, as well as a novel analog computer to show that complex absorption curves in living plants are produced by a number of distinct forms of chlorophyll occurring in vivo. French used the Blinks rate-measuring oxygen electrode to measure action spectra of oxygen evolution by photosynthesis automatically. He and Jack Myers did some of the pioneering work on the Emerson effect showing the necessary cooperation of two photosystems in photosynthesis. French used the Carnegie Institution's fellowship program to bring large numbers of scientists from around the world to his laboratory. When Stacy French died in 1995, the field of photosynthesis lost one of its great and early pioneers.This is CIW/DPB publication No. 1314. 相似文献
953.
954.
Prakash Bhuta Jiří Žemclička 《Biochimica et Biophysica Acta (BBA)/General Subjects》1985,841(2):145-150
The (1c) and (2c), obtained by chemical synthesis, are substrates for ribosomal peptidyltransferase from Escherichia coli. Nucleoside 1c, which mimics an anti conformation of antibiotic formycin, has 80% of the acceptor activity of puromycin at 5 · 10?4 M determined by the release of N-Ac-Phe residue from the 70 S complex. The reaction product, (1d), was characterized by paper electrophoresis before and after alkaline hydrolysis. By contrast, nucleoside 2c, which resembles a syn conformation of formycin, exhibited only 20% of the acceptor activity of puromycin at 5 · 10∮4 M and essentially none in the concentration region between 1 · 10?6 and 1 · 10?4 M. The results which are in accord with previous models have shown that a substrate with its base in an anti conformation is preferable for the acceptor site of peptidyltransferase than the corresponding syn counterpart, Nevertheless, it is possible that an intermediate conformation, for example, high anti (amphi-minus), is an optimal arrangement for acceptor site substrates. 相似文献
955.
Motomi Katada Somdev Tyagi Amar Nath Richard L. Petersen Raj K. Gupta 《Biochimica et Biophysica Acta (BBA)/General Subjects》1979,584(1):149-163
A new isomeric form of cobalamins is reported. The conversion of cobalamin to cobalamin′ (the new form) is achieved by substituting the benzimidazole base by a less bulky group like H2O of CN? and modest thermal treatment. The back conversion of adenosylcobalamin′ to the corresponding regular form occurs in the ‘base-off’ form at room temperature. It seems that the corrin ring becomes quite flexible in the ‘base-off’ form and the freer axial movement of the cobalt atom flips the corrin ring into a different conformation. The change in conformation is borne out by subtle changes in the proton magnetic resonances on the corrin ring and the base, and very marked variation in the emission Mössbauer spectra. The latter is indicative of appreciable changes in the spatial conformation in the immediate vicinity of the central metal atom.The ultraviolet-visible and infrared spectra of a cobalamin′ are indistinguishable from those of its corresponding regular form.The new conformational isomeric species is present as an impurity in all commercially available cobalamins (including pharmaceutical preparations). It raises the question whether the cobalamins′ constitute the real biologically active anti-anemic factor in humans 相似文献
956.
Ferdinand Bohlmann Lakshmi N. Dutta Werner Knauf Harold Robinson Robert M. King 《Phytochemistry》1980,19(3):433-436
The investigation of Aster umbellatus afforded six new eudesmanolides and a new selina-triene and from Aster exilis a new tremetone derivative was isolated. The structures were elucidated by spectroscopic methods and by some chemical transformations. The chemotaxonomic situation is discussed briefly. 相似文献
957.
An impermeant benzodiazepine receptor ligand was prepared by derivatization of the aminobenzodiazepine 1012-S with 4-sulfophenylisothiocyanate. The resulting N-(4-sulfophenyl)-thiocarbamoyl derivative of 1012-S (SPTC-1012S) was purified by reverse-phase HPLC, and the predicted structure was verified by mass spectrometry. The apparent affinity of SPTC-1012S (IC50 = 9.8 +/- 2.9 nM) for displacement of [3H]flunitrazepam from intact chick cortical neurons was similar to that of 1012-S (IC50 = 4.0 +/- 0.3 nM). However, at concentrations from 0.1 to 10 microM, 1012-S was consistently more efficacious than SPTC-1012S, a finding indicating that 6-8% of the benzodiazepine receptor pool was not accessible to the impermeant compound. This inaccessible pool was eliminated by permeabilization of the cells with saponin or Triton X-100, a result suggesting that approximately 7% of neuronal benzodiazepine receptors are intracellular. Acute treatment (1-4 h at 37 degrees C) of neurons with 100 microM gamma-aminobutyric acid (GABA) or 100 nM clonazepam had little effect on the level of [3H]flunitrazepam binding but increased the proportion of intracellular receptors by 61 and 74%, respectively, compared with untreated controls. Similar treatment with 1 mM GABA increased the level of intracellular sites by 154-176%. The effect of GABA on receptor internalization was blocked by cotreatment with the GABAA receptor antagonist R 5135. The results suggest that SPTC-1012S can be used as a probe to study the internalization of the GABAA/benzodiazepine receptor complex under normal conditions or following acute or chronic treatment with agonists. 相似文献
958.
959.
Previously, we reported a [99mTc(ǀ)]+ labeled d-glucoamine derivative (99mTc-CN5DG) and evaluated it as a tumor imaging agent in mice bearing A549 tumor xenografts. In this paper, 99mTc-CN5DG was further studied in U87 MG (human glioma cells), HCT-116 (human colon cancer cells), PANC-1 (human pancreatic cancer cells) and TE-1 (human esophageal cancer cells) tumor xenografts models to verify its potential application for imaging of different kinds of tumors. The biodistribution data showed that 99mTc-CN5DG had a similar biodistribution pattern in four tumor models at 2 h post-injection with high accumulation in tumors and kidneys. The tumor/muscle ratios (from 4.08 ± 0.42 to 9.63 ± 3.53) and tumor/blood ratios (from 17.18 ± 7.40 to 53.17 ± 16.16) of 99mTc-CN5DG in four tumor models were high. All four kinds of tumors could be clearly seen on their corresponding SPECT/CT images. Pharmacokinetic study in healthy CD-1 mice demonstrated that 99mTc-CN5DG cleared fast from blood (2 min, 12.97 ± 0.88%ID/g; 60 min, 0.33 ± 0.06%ID/g) and the blood distribution, elimination half-life was 5.81 min and 21.16 min, respectively. No abnormality was observed through the abnormal toxicity study. All of the above results demonstrated that 99mTc-CN5DG could be a broad-spectrum SPECT probe for tumor imaging and its further clinical application is warranted. 相似文献