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231.
Aidan L. McParland Taylor L. Follansbee Geoffrey K. Ganter 《Journal of visualized experiments : JoVE》2015,(98)
Drosophila larvae are used in many behavioral studies, yet a simple device for measuring basic parameters of larval activity has not been available. This protocol repurposes an instrument often used to measure adult activity, the TriKinetics Drosophila activity monitor (MB5 Multi-Beam Activity Monitor) to study larval activity. The instrument can monitor the movements of animals in 16 individual 8 cm glass assay tubes, using 17 infrared detection beams per tube. Logging software automatically saves data to a computer, recording parameters such as number of moves, times sensors were triggered, and animals’ positions within the tubes. The data can then be analyzed to represent overall locomotion and/or position preference as well as other measurements. All data are easily accessible and compatible with basic graphing and data manipulation software. This protocol will discuss how to use the apparatus, how to operate the software and how to run a larval activity assay from start to finish. 相似文献
232.
Angela M. O'Connor Thomas J. Burton Catherine A. Leamey Atomu Sawatari 《Journal of visualized experiments : JoVE》2014,(94)
Environmental enrichment can dramatically influence the development and function of neural circuits. Further, enrichment has been shown to successfully delay the onset of symptoms in models of Huntington’s disease 1-4, suggesting environmental factors can evoke a neuroprotective effect against the progressive, cellular level damage observed in neurodegenerative disorders. The ways in which an animal can be environmentally enriched, however, can vary considerably. Further, there is no straightforward manner in which the effects of environmental enrichment can be assessed: most methods require either fairly complicated behavioral paradigms and/or postmortem anatomical/physiological analyses. This protocol describes the use of a simple and inexpensive behavioral assay, the Puzzle Box 5-7 as a robust means of determining the efficacy of increased social, sensory and motor stimulation on mice compared to cohorts raised in standard laboratory conditions. This simple problem solving task takes advantage of a rodent’s innate desire to avoid open enclosures by seeking shelter. Cognitive ability is assessed by adding increasingly complex impediments to the shelter’s entrance. The time a given subject takes to successfully remove the obstructions and enter the shelter serves as the primary metric for task performance. This method could provide a reliable means of rapidly assessing the efficacy of different enrichment protocols on cognitive function, thus paving the way for systematically determining the role specific environmental factors play in delaying the onset of neurodevelopmental and neurodegenerative disease. 相似文献
233.
Ognean T 《Biotechnology and bioengineering》1993,41(11):1014-1020
The effect of power consumption on oxygen transfer efficiency has been evaluated for both surface and subsurface aeration systems. Two new dimensionless criteria (numbers) have been proposed for this purpose. They are termed the efficiency criterion or T(o) number and the power consumption effect criterion or Av number. The analysis presented in this article is based on data obtained on various full-scale aeration systems, and it shows that for each aerator there is an optimum power consumption at which maximum oxygen transfer efficiency results. The oxygen transfer efficiency has maximum values when the efficiency criterion (T(o) number) reaches an approximately constant value. The results have proven that when there are several aerators with identical diameters and the same rotational velocity but different geometrical shapes, the most efficient one will be (after having reached the maximum transfer efficiency) the one with the highest power consumption. For two aerators varying in diameter but with the same parameter as above (maximum transfer, equal rotational velocity), different efficiencies are obtained, the difference being a function of d(-0.5). The results of investigating subsurface aeration systems show that the efficiency of decreased. 相似文献
234.
表型单元、形态种和生物种——关于化石种判别问题的探讨 总被引:9,自引:4,他引:5
目前在我国古生物学研究中存在着许多有关物种判别的问题。在相当程度上这是与居群概念的贫乏和传统的林奈式类型-形态学研究方法的影响分不开的。广泛存在于物种间的形态间断为识别物种带来了方便,但它与生殖隔离并不完全吻合。若只考虑形态特征,所判定的化石种大多只相当于表型单元。本文探讨如何将形态标准与居群、群落-生态和时空等项标准相结合来区分物种与表型单元。以上这些标准分别反映了物种的生物学特性的不同方面,而生殖一遗传标准是将它们有机地联系在一起的共同基础。古生物学必须站在理性的高度上俯瞰生命发展过程中的物种,从现代生物学的知识库中汲取养料。化石种研究现状的改观尚有待于我们付出艰巨的努力。通过这种努力将使古生物学进入一个新的、更严格的科学发展阶段。 相似文献
235.
Summary
Tibicen chiricahua and T. duryi are cicada species that are active as adults early each summer in central New Mexico, and are often syntopic in pinyon-juniper woodlands. Both species regulate thoracic temperature (Tth) within fairly narrow limits by utilizing behavioral mechanisms and evaporative cooling. However, syntopic populations of these two species were found to regulate at different Tth despite having synchronous annual and daily activity periods; overall mean Tth of T. chiricahua was 3.1°C higher than it was for T. duryi. Interspecific differences in evaporative cooling abilities and rates of passive heat exchange could not account for this difference in Tth. Part of the difference in Tth resulted from the fact that individuals of the two species were active in thermally distinct microhabitats. Within each species, mean Tth varied among behavior categories, and differences in how the two species allocated their time between activities also contributed to the interspecific difference in Tth. Though T. duryi is restricted to pinyon-juniper habitats such as the one in this study, T. chiricahua is also found in warmer habitats. The difference in Tth in the syntopic populations probably reflects interspecific differences in thermal preferences and thermal optima that are adaptive over their respective habitat ranges. The degree of dependence of Tth on ambient temperature (Tam) varied between activities within both species, with the least dependence exhibited during singing. Singing involves intense activity of tymbal muscles, which apparently can only function effectively over a relatively narrow range of temperature. 相似文献
236.
Sharon A. Klinger John J. Magnuson George W. Gallepp 《Environmental Biology of Fishes》1982,7(2):113-120
Synopsis Three species (central mudminnow, fathead minnow and brook stickleback) survive when a northern Wisconsin lake becomes anoxic in winter. Some gas bubbles beneath the ice contained as much as 11° oxygen when the lake water contained <0.30 mg 1–1 dissolved oxygen. Experiments conducted in the field determined that gas bubbles prolonged survival of all species, especially the mudminnow and stickleback. In the laboratory, brook sticklebacks exhibited the lowest and fathead minnows the highest routine metabolic rate corrected for weight. Rate of gill ventilation of all three increased from 20 to 70 beats per minute as oxygen levels declined from 4.0 to 0.25 mg 1–1. At low oxygen levels they moved to the upper one-third of the test tanks. Small size, low metabolic rate, tolerance of low oxygen conditions and reduced activity resulted in reduced demand for dissolved oxygen. Head shape, ventilation rate, vertical movement and utilization of high oxygen microzones also enhanced exploitation of low levels of dissolved oxygen. Central mudminnows used oxygen directly from gas bubbles found under the ice. 相似文献
237.
A Radioimmunoassay for Ependymins β and γ: Two Goldfish Brain Proteins Involved in Behavioral Plasticity 总被引:4,自引:4,他引:0
Abstract: A radioimmunoassay (RIA) using 125 I-labeled antigen was developed for the quantitative determination of two goldfish brain proteins (ependymins β and γ). The proteins were isolated from the cerebrospinal fluid (CSF) and cells of the ependymal zone surrounding goldfish brain ventricles. The turnover rates of β and γ were previously shown to be specifically enhanced after the animals successfully acquired a new pattern of swimming behavior. Femtomole quantities of ependymin β were measurable by the RIA. In applications of the assay, β and γ ependymins were found to have common immunological properties, since 125 I-β-antigen bound to antibody could be displaced by unlabeled ependymin γ as well as ependymin β but not by a variety of other proteins including several purified glycoproteins isolated from goldfish brain. The ependymins were shown to constitute 14% of the total protein content of the brain extracellular fluid and also to be present as a minor component of the serum proteins (0.3%). Ependymins β and γ have an immunological reactivity in these fractions that can be increased by a factor of 30 on heating. The data suggest that the antigenicity of the molecules is highly masked, and that it may require some unraveling of the quaternary structure of the proteins before maximal interaction with the antisera becomes possible. 相似文献
238.
Forced swimming in warm or cold water can lead to immobility and analgesia in mice. In this report we demonstrate that the peptide MIF-1 (Pro-Leu-Gly-NH2) was able to attenuate the analgesia induced by swimming in warm water, but not that induced by swimming in cold water. In addition, we show that the analgesia and the immobility may be differentially mediated since MIF-1 was able to reduce the immobility at doses different from those necessary to reduce the analgesia. These results confirm previous research indicating that MIF-1 may act as an anti-opiate and further demonstrate that MIF-1 affects analgesia induced by stress. 相似文献
239.
Behavioral effects of lysin-vasopressin (LVP) were investigated applying two paradigms from human experimental psychology. The first task was designed to simulate amnesic symptoms in normals. The second task addressed the emotional value of the items to be processed. Additionally, EEG recordings were used as indicators of the central nervous system effectiveness of LVP. Blood pressure and heart rate measured peripheral arousal. The co-twin control method was employed to increase experimental power. Contrary to the prediction of the vasopressin memory hypothesis none of the specific memory parameters was improved by LVP treatment. Changes in the electrical activity of the brain, but not in blood pressure and heart rate indicated central nervous system actions of LVP. However, interpretation of LVP effects in terms of memory processing seems not to be justified. 相似文献
240.