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841.
R Donato 《Biochemical and biophysical research communications》1984,122(3):983-990
We have examined the S-100-chlorpromazine interplay at the level of brain microtubule proteins in vitro. The results indicate that in the presence of 0.12 M KCl and 10 microM free Ca2+ the inhibitory effect of S-100 on microtubule assembly is additive to that of chlorpromazine, but S-100 fails to potentiate the disassembling effect of 0.1 mM Ca2+ if added to assembled microtubule proteins after chlorpromazine and Ca2+, probably because of inhibition of S-100 by the phenothiazine. Chlorpromazine does not compete with S-100 for binding to purified tubulin. 相似文献
842.
The molecular structure of aldehydes is closely related to their antimicrotubular effect. Morphological modifications of the microtubular system in living cells after incubation with certain aldehydes are consistent with biochemical alterations detected in previous research. The microtubular arrangement was visualized by an immunofluorescence technique with antitubulin antibodies, while the content of tubulin in the cells was evaluated by a colchicine binding assay. 2-Nonenal behaved similarly to 4-hydroxynonenal, a lipid peroxidation product, disorganizing microtubular network in 3T3 fibroblasts and decreasing the amounts of tubulin able to bind labelled colchicine. Nonanal did not significantly impair the tubulin characteristics in the cells, despite the fact that it has been shown to be active on the purified microtubular system; benzaldehyde was ineffective. This would appear to explain the mechanisms of interaction of aliphatic aldehydes which might be suitable for use as antimicrotubular drugs. 相似文献
843.
Nisha E. Thomas Sasidharan Shashikala Suparna Sengupta 《Journal of cellular biochemistry》2010,110(6):1334-1341
The newer member of the tubulin superfamily, γ‐tubulin, is known to mediate microtubule nucleation from the centrosome of eukaryotic cells with the aid of some other proteins. The major amount of γ‐tubulin is believed to be located in the centrosome before the onset of mitotic division. However, a considerable amount has been found in the cytoplasm in the form of a complex whose function is not well known. Microtubules are most abundant in brain tissues and brain microtubules have been extensively used in many in vitro studies. Thus, it is relevant to use brain tissue to characterize cytoplasmic γ‐tubulin complex. Here we show that cytoplasmic γ‐tubulin in brain tissues exists as a ring complex as in other tissues. Interestingly, along with the common members of the γ‐TuRC reported from several tissues and species, the purified brain cytoplasmic complex contains some high molecular weight proteins including α and β nonerythroid spectrin which are not found in other tissues. Immunohistochemical studies of brain tissue sections also show the co‐localization of γ‐tubulin and spectrin. The possible implications have been discussed. J. Cell. Biochem. 110: 1334–1341, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
844.
Summary The microtubules in different parts of the neuron and synaptosomes were examined with respect to their stability, structure and orientation. On the basis of distribution, different labilities and differences in protofilament substructure seen by tannic acid staining, we have classified microtubules into eight major categories. Functional involvements in vesicle translocation, cytoskeletal support and the regulation of assembly/disassembly are considered.Dr. L.E. Westrum is an affiliate of the CDMRC at the University of Washington and a recipient of a Wellcome Research Travel Grant from the Burroughs-Wellcome Fund. The research was also supported in part by NIH Grants NS 09678, NS 04053 (NINCDS) and DE 04942 (NIDR), DHHS 相似文献
845.
P A Theodoropoulos A Gravanis I Saridakis C Stournaras 《Cell biochemistry and function》1992,10(4):281-288
In the present study, using immunofluorescence microscopy, we have demonstrated that normal and Ha-ras-1 transformed Buffalo rat liver (BRL) cells which were exposed to cytoskeletal protein inhibitors, showed a differential resistance of their microfilament and microtubule networks. One hour exposure of normal BRL cells to 10(-5) M cytochalasin B provoked a clear and already total breakdown of actin filaments. However, at this concentration of cytochalasin B, the microfilaments of transformed BRLHO6T1-1 cells were not seriously affected; a higher cytochalasin B concentration (> or = 2 x 10(-5) M) was required to induce a significant breakdown of microfilaments in these transformed cells. The two cell lines also demonstrated differential microtubule stability when they were treated with either colchicine or triethyllead. Three hours exposure to 10(-6) M of either antimicrotubule agents was sufficient to disrupt the microtubules of normal BRL cells, without affecting their counterparts in the transformed BRLHO6T1-1 cells. A 10-fold higher drug concentration (10(-5) M) was required to induce microtubular breakdown in the transformed BRL cells. The differential stability of microfilaments and microtubules in normal and transformed BRL cells that was observed could not be attributed to a differential internalization of the agents, as shown by experiments on the uptake of [3H]-cytochalasin B and triethyllead. In addition, the transformed BRLHO6T1-1 cells did not express altered actin and tubulin isoforms, as demonstrated by isoelectric focusing followed by immunoblotting analysis. We conclude that the transformation of BRL cells with the Ha-ras-1 oncogene results in a greater stability of microfilaments and microtubules, leading to a structurally firmer cell shape.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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