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811.
812.
Céline Cuche Anne Danckaert Maria‐Isabel Thoulouze Fabrice de Chaumont Nathalie Perrault Jean‐Christophe Olivo‐Marin Sandrine Etienne‐Manneville Monique Arpin Vincenzo Di Bartolo Andrés Alcover 《The EMBO journal》2010,29(14):2301-2314
T‐cell receptor (TCR) signalling is triggered and tuned at immunological synapses by the generation of signalling complexes that associate into dynamic microclusters. Microcluster movement is necessary to tune TCR signalling, but the molecular mechanism involved remains poorly known. We show here that the membrane‐microfilament linker ezrin has an important function in microcluster dynamics and in TCR signalling through its ability to set the microtubule network organization at the immunological synapse. Importantly, ezrin and microtubules are important to down‐regulate signalling events leading to Erk1/2 activation. In addition, ezrin is required for appropriate NF‐AT activation through p38 MAP kinase. Our data strongly support the notion that ezrin regulates immune synapse architecture and T‐cell activation through its interaction with the scaffold protein Dlg1. These results uncover a crucial function for ezrin, Dlg1 and microtubules in the organization of the immune synapse and TCR signal down‐regulation. Moreover, they underscore the importance of ezrin and Dlg1 in the regulation of NF‐AT activation through p38. 相似文献
813.
Cdc34/Ubc3 is a ubiquitin-conjugating enzyme that functions in targeting proteins for proteasome-mediated degradation at the G1 to S cell cycle transition. Elevation of Cdc34 protein levels by microinjection of bacterially expressed Cdc34 into mammalian cells at prophase inhibited chromosome congression to the metaphase plate with many chromosomes remaining near the spindle poles. Chromosome condensation and nuclear envelope breakdown occurred normally, and chromosomes showed oscillatory movements along mitotic spindle microtubules. Most injected cells arrested in a prometaphase-like state. Kinetochores, even those of chromosomes that failed to congress, possessed the normal trilaminar plate ultrastructure. The elevation of Cdc34 protein levels in early mitosis selectively blocked centromere protein E (CENP-E), a mitotic kinesin, from associating with kinetochores. Other proteins, including two CENP-E-associated proteins, BubR1 and phospho-p42/p44 mitogen-activated protein kinase, and mitotic centromere-associated kinesin, cytoplasmic dynein, Cdc20, and Mad2, all exhibited normal localization to kinetochores. Proteasome inhibitors did not affect the prometaphase arrest induced by Cdc34 injection. These studies suggest that CENP-E targeting to kinetochores is regulated by ubiquitylation not involving proteasome-mediated degradation. 相似文献
814.
Mariya Genova Lenka Grycova Verena Puttrich Maria M Magiera Zdenek Lansky Carsten Janke Marcus Braun 《The EMBO journal》2023,42(5)
Tubulin posttranslational modifications have been predicted to control cytoskeletal functions by coordinating the molecular interactions between microtubules and their associating proteins. A prominent tubulin modification in neurons is polyglutamylation, the deregulation of which causes neurodegeneration. Yet, the underlying molecular mechanisms have remained elusive. Here, using in‐vitro reconstitution, we determine how polyglutamylation generated by the two predominant neuronal polyglutamylases, TTLL1 and TTLL7, specifically modulates the activities of three major microtubule interactors: the microtubule‐associated protein Tau, the microtubule‐severing enzyme katanin and the molecular motor kinesin‐1. We demonstrate that the unique modification patterns generated by TTLL1 and TTLL7 differentially impact those three effector proteins, thus allowing for their selective regulation. Given that our experiments were performed with brain tubulin from mouse models in which physiological levels and patterns of polyglutamylation were altered by the genetic knockout of the main modifying enzymes, our quantitative measurements provide direct mechanistic insight into how polyglutamylation could selectively control microtubule interactions in neurons. 相似文献
815.
816.
Tomoki Yano Kazuto Tsukita Hatsuho Kanoh Shogo Nakayama Hiroka Kashihara Tomoaki Mizuno Hiroo Tanaka Takeshi Matsui Yuhei Goto Akira Komatsubara Kazuhiro Aoki Ryosuke Takahashi Atsushi Tamura Sachiko Tsukita 《The EMBO journal》2021,40(2)
Apical constriction is critical for epithelial morphogenesis, including neural tube formation. Vertebrate apical constriction is induced by di‐phosphorylated myosin light chain (ppMLC)‐driven contraction of actomyosin‐based circumferential rings (CRs), also known as perijunctional actomyosin rings, around apical junctional complexes (AJCs), mainly consisting of tight junctions (TJs) and adherens junctions (AJs). Here, we revealed a ppMLC‐triggered system at TJ‐associated CRs for vertebrate apical constriction involving microtubules, LUZP1, and myosin phosphatase. We first identified LUZP1 via unbiased screening of microtubule‐associated proteins in the AJC‐enriched fraction. In cultured epithelial cells, LUZP1 was found localized at TJ‐, but not at AJ‐, associated CRs, and LUZP1 knockout resulted in apical constriction defects with a significant reduction in ppMLC levels within CRs. A series of assays revealed that ppMLC promotes the recruitment of LUZP1 to TJ‐associated CRs, where LUZP1 spatiotemporally inhibits myosin phosphatase in a microtubule‐facilitated manner. Our results uncovered a hitherto unknown microtubule‐LUZP1 association at TJ‐associated CRs that inhibits myosin phosphatase, contributing significantly to the understanding of vertebrate apical constriction. 相似文献
817.
Tomoko Satake Atsushi Suzuki Tomoko Satake Kazunari Yamashita Kenji Hayashi Satoko Miyatake Miwa Tamura-Nakano Hiroshi Doi Yasuhide Furuta Go Shioi Eriko Miura Yukari H Takeo Kunihiro Yoshida Hiroyuki Yahikozawa Naomichi Matsumoto Michisuke Yuzaki Atsushi Suzuki 《The EMBO journal》2017,36(9):1227-1242
The axon initial segment (AIS) is a specialized domain essential for neuronal function, the formation of which begins with localization of an ankyrin-G (AnkG) scaffold. However, the mechanism directing and maintaining AnkG localization is largely unknown. In this study, we demonstrate that in vivo knockdown of microtubule cross-linking factor 1 (MTCL1) in cerebellar Purkinje cells causes loss of axonal polarity coupled with AnkG mislocalization. MTCL1 lacking MT-stabilizing activity failed to restore these defects, and stable MT bundles spanning the AIS were disorganized in knockdown cells. Interestingly, during early postnatal development, colocalization of MTCL1 with these stable MT bundles was observed prominently in the axon hillock and proximal axon. These results indicate that MTCL1-mediated formation of stable MT bundles is crucial for maintenance of AnkG localization. We also demonstrate that Mtcl1 gene disruption results in abnormal motor coordination with Purkinje cell degeneration, and provide evidence suggesting possible involvement of MTCL1 dysfunction in the pathogenesis of spinocerebellar ataxia. 相似文献
818.
《Molecular cell》2023,83(13):2290-2302.e13
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819.
820.
Microtubule involvement in diatom valve symmetry and pattern formation was investigated using cells synchronized subsequent to mitosis and cytokinesis but prior to cell wall formation. Two analog drug pairs, each consisting of an active and an inactive microtubule drug, were used to distinguish inhibitory effects related or unrelated to microtubule disruption. The active anti-microtubule drug of each analog pair produced significantly higher percentages of aberrant valves than did the respective inactive analogs. High frequencies of aberrant valves also were caused by N-isophenlpropylcarbamate, which disorganizes rather than disrupts microtubules. Valves could be placed into different classes based upon characteristic aberrations. Formation of these classes was not random but was instead a function of both the drug and the drug concentration. The central nodule and the raphe were the principal valve components affected by anti-microtubule drugs. Stria alterations appeared as a secondary result of alterations in the central nodule/raphe. Valve aberrations occurred at very low drug concentrations in the range 1 × 10?6 to 1 × 10?9M. 相似文献