Update on the possible mode of action of the jasmonates: Focus on the metabolism of cell wall polysaccharides in relation to growth and development

Jasmonic acid (JA) and its related compounds (jasmonates) applied to plant tissues exert either inhibitory or promotive effects in growth and developmental processes, which in some ways are similar to abscisic acid. However, little is known about the mode of action of the jamonates at the tissue or organ levels. Here, we review partial evidence for the physiological action of the jasmonates on cell elongation and abscission.
Jasmonates inhibit the IAA-induced cell elongation of oat coleoptile segments not by affecting energy production, osmoregulation and cell wall loosening, but by inhibiting the synthesis of cell wall polysaccharides. The inhibition is partially reversed by simultaneous application of sucrose. Inhibition of IAA-induced elongation by JA is only observed in monocotyledons, not in dicotyledons. These effects suggest that jasmonates exert their inhibitory effect on cell elongation by affecting the metabolism of the cell wall polysaccharides in monocotyledons.
Jasmonates promote the abscission of bean petiole explants without enhancing ethylene production. Cells in the petiole adjacent to the abscission zone expand during abscission. In the abscission zone, jasmonates decrease the amount of cellulosic but not that of noncellulosic polysaccharides. Jasmonates increase the activities of cellulase and decrease the levels of UDP-sugars, which are important intermediates for the synthesis of cell wall polysaccharides in the abscission zone, probably resulting in the decreased level of cellulose and the mechanical weakness of cell walls.
Thus, it is suggested that jasmonates exert their multiple physiological effects by affecting the metabolic processes of cell wall polysaccharides.     

Binding of phytochrome to plasma membranes in vitro

Nicotiana tabacum (cv. Xanthi), N. plumbaginifolia Viviani, N. sylvestris Speg. and Comes and Petunia axillaris × ( P. axillaris × P. hybrida ) (cv. Mitchell) mesophyll protoplast-derived cells were able to grow at low densities in chemically-defined media. Protoplasts of different origins (mesophyll, epidermis, pith, suspension culture) also gave rise to protoplast-derived cells that were able to grow at low densities. When auxin requirements at low densities were compared for different sources of auxins, IAA was found to be efficient in the same range of concentrations as NAA. This was unexpected since tobacco mesophyll protoplasts cannot be induced to divide when plated at high density in the presence of IAA. Optimal 2,4-D concentrations for low density growth were higher and clearly pH-dependent. On the contrary, picloram induced low density growth over a wide range of concentrations suggesting a distinct mechanism of action. These results confirm and extend previous observation on the tobacco mesophyll protoplast and show that the low-density growth technique has a potential use for the study of the action of phytohormones.     

Sex ratio of the cyclic parthenogen Daphnia magna in a variable environment

The relationship between environmental factors, sex ratio and mating system in Daphnia magna was examined, and the adaptiveness of environmental sex determination over ametic sex determination was explored. Monthly sexual sex ratios (males over total number of males and sexual females) ranged from 0.31 to 1.0, the three-year average equalling 0.61. However, if only the samples collected during the period of frequent sexuality from August to October are included, the sexual sex ratio becomes equal, 0.51. Sexual sex ratios varied real between samples during the same period and the standard errors appeared highest in July ad August. Typical of suck times is some uncertainty in the environment, and the environmental cues can be contradictory. Sex expression in Daphnia appears to be determined by responses to complicated interactions between different environmental factors, which adaptively alter the sex ratio. The longterm sexual sex ratio of Daphnia aproaching the equilibrium 1:1, despite environmental sex %termination, gives support for Fisier's classic theory of equal parental investment in both sexes. An equal sex ratio is advantageous also during periods of small population size because it maximizes the effective population size.     

Canola and Oat Forage Potential Evaluation in Four Early Planting Dates

Canola and oat forage potential may be affected by climatic conditions when sown early. The objective of this study was to evaluate the forage canola and oat potential in four early sowing dates (September 11 and 25; October 9 and 23) during the 2012-2013 and 2013-2014 cycles in Matamoros, Coahuila, Mexico. Growth cycle duration, chemical composition, dry matter (DM), crude protein (CP), and net energy for lactation (NE_L) yields were determined. High temperatures and long photoperiods affected crops seeded on September 11, accelerating growth and reducing canola (26.6%-31.7%) and oat (15.8%) DM yields. As of September 25, canola cv IMC 205 reached DM yields (7746 kg ha^-1 -9276 kg ha^-1 ) similar to those obtained by oat (8115 kg ha^-1 -9507 kg ha^-1 ), while canola cv Hyola 401 obtained such yields only until October 23. Canola chemical composition was better than that found in oat, with higher CP, but lower acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents. Canola equaled oat CP yields (972 kg ha^-1 -1215 kg ha^-1 ) in the first sowing date, while in the other three other canola sowings reached higher yields (1193 kg ha^-1 -1889 kg ha^-1 ). As for NEL yields, no difference was observed between both species. The best sowing date for canola is from September 25 on, with CP production advantages over oat.     

Interactions between Heterodera avenae and Pratylenchus neglectus on Wheat

In a long-term field experiment, differential population densities of Heterodera avenae were produced by frequent cropping with resistant (cv. Panema) or susceptible (cv. Peniarth) oat. The two oat cultivars were equally good hosts of Pratylenchus neglectus in a glass house experiment with field soil. On wheat crops grown after oats in field experiments, P. neglectus population densities in roots were higher in plots where H. avenae had been controlled than in plots with moderate infestations (40 H. avenae eggs/g soil). The field observations indicated that the reduction in population densities of P. neglectus coincided with the development in roots of sedentary stages of the cyst nematode. Evidence for an indirect effect of H. avenae on P. neglectus was found in vitro in a split-root experiment. In the same field, grain yields of two wheat cultivars susceptible or resistant to H. avenae, but both susceptible to P. neglectus, was not reduced by P. neglectus. Alternation of H. avenae resistant and susceptible cultivars is a possible way of exploiting the inverse relationship between these nematodes, whilst controlling cyst nematode -populations in intensive cereal production systems.     

Abscisic Acid and Ethylene Increase in Heterodera avenae-infected Tolerant or Intolerant Oat Cultivars

The relationship between root stunting caused by the cereal cyst nematode and levels of two root growth inhibiting hormones, abscisic acid and ethylene, was investigated in aseptically cultured root segments and in intact roots of two oat cultivars differing in tolerance to the nematode. Cultured root segments of oat cultivars New Zealand Cape (tolerant) and Sual (intolerant) were inoculated with sterilized Heterodera avenae second-stage juveniles. Suppressed growth of root axes and emerged laterals following nematode penetration corresponded to an increase in abscisic acid and ethylene in roots of both intolerant and tolerant cultivars. When the experiment was repeated on intact root systems, nematodes retarded root growth of Sual more than New Zealand Cape despite an increase in ABA and ethylene in both cultivars. Abscisic acid and (or) ethylene may be involved in growth inhibition of H. avenae-infected roots but appear to play no direct role in determining tolerance.     

Avena fatua L. seed and seedling nutrient dynamics as influenced by mycorrhizal infection of the maternal generation

Abstract. The objective of this study was to determine how mycorrhizal infection of one generation of plants influences the nutrient dynamics of seeds and seedlings comprising the subsequent generation. We showed that, for Avena fatua L., seeds produced by mycorrhizal (M) plants consistently contained significantly more phosphorus (particularly the phytate P and residual P fractions) than seeds produced by non-mycorrhizal (NM) plants. We also followed the development of spikelets produced by M and NM plants. The rates of increase in spikelet dry weight and nitrogen content were largely unaffected by mycorrhizal infection. However, the rate of P accumulation into spikelets was significantly increased by mycorrhizal infection. Greater endosperm P reserves in seeds produced by M plants were associated with greater rates of P accumulation in resultant seedlings. Moreover, offspring plants (all NM) produced by M mother plants had significantly higher root and rhizosphere phosphatase, ATPase and phytase activities than offspring plants produced by NM mother plants. This persistent maternal effect has never before been described. Our results suggest that mycorrhizal infection of one generation of plants may have substantial positive effects on the offspring generation, and thus, may influence plant population dynamics.