全文获取类型
收费全文 | 354篇 |
免费 | 14篇 |
国内免费 | 12篇 |
出版年
2024年 | 1篇 |
2023年 | 6篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 8篇 |
2018年 | 11篇 |
2017年 | 18篇 |
2016年 | 7篇 |
2015年 | 19篇 |
2014年 | 24篇 |
2013年 | 24篇 |
2012年 | 14篇 |
2011年 | 23篇 |
2010年 | 10篇 |
2009年 | 25篇 |
2008年 | 31篇 |
2007年 | 29篇 |
2006年 | 31篇 |
2005年 | 10篇 |
2004年 | 14篇 |
2003年 | 16篇 |
2002年 | 13篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
排序方式: 共有380条查询结果,搜索用时 31 毫秒
71.
72.
目的:通过研究三氧化二砷(AS2O3)对人结肠癌细胞株LS-174T在体内外实验中增殖情况的影响,探讨As2O3治疗结肠癌的机制。方法:1、试验分组:体外细胞培养及体内动物实验均分三组:对照组,As2O3低剂量组,As2O3高剂量组。2、MTT法测定细胞增殖数的情况。3、丫啶橙/溴乙啶(AO/EB)双荧光染色检测细胞凋亡率。4、免疫组织化学法测定结肠癌细胞和裸鼠实体肿瘤中HIF-1α和VEGF的表达。5、测量各组裸鼠实体肿瘤的重量及体积。结果:体外试验中,由MTT法可以看出,As2O3治疗组细胞生存率明显低于对照组(P〈0.01),其中AS2O3高剂量组明显低于低剂量组。双荧光染色结果As2O3高剂量组细胞凋亡百分率明显高于低剂量组和对照组(P〈0.01)。免疫组化结果显示结肠癌细胞HIF-1α和VEGF在对照组的阳性表达率明显高于As2O3治疗组(P〈0.01),低剂量组高于高剂量组(P〈0.05)。体内实验中,治疗组平均瘤重和瘤体积均较对照组明显减低,(P〈0.01)。HIF-1α及VEGF在裸鼠肿瘤组织中均有阳性表达,其中对照组较AS2O3治疗组高(P〈0.01)。结论:As2O3能够抑制体内外人结肠癌细胞的生长,并能明显抑制HIF-1α和VEGF的表达,从而抑制肿瘤的生长。 相似文献
73.
Trace Element Composition of Selected Fertilizers Used in Chile: Phosphorus Fertilizers as a Source of Long-Term Soil Contamination 总被引:2,自引:0,他引:2
Mauricio Molina Fabián Aburto Raúl Calderón Marcia Cazanga Mauricio Escudey 《Soil & Sediment Contamination》2009,18(4):497-511
Anthropogenic activities like agriculture have resulted in increased concentrations of some trace elements of toxicological and environmental concern in soils. Application of fertilizers has been one of the major inputs of these contaminants to agricultural soils in developing countries. Twenty-two fertilizers, including straight nitrogen (N), phosphorus (P), potassium (K), and NK fertilizers and micronutrient sources, were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES) for arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), nickel (Ni), lead (Pb), vanadium (V), and zinc (Zn). As expected, the trace element content of fertilizers was highly variable and related to the origin of the material. Phosphorus fertilizers, especially triple superphosphate, presented the highest As, Cd, Cu, Cr, Ni, V, and Zn concentrations. In some of these fertilizers, the Cr, V, and Zn contents reached values greater than 3475 mg kg?1 of P, and the Cd content (up to 288 mg kg?1 of P) was several times higher than the regulatory limits of different countries. Some micronutrient sources presented the highest concentrations of Mn and Pb. In the cases of N, K, and NK fertilizers, the trace element concentration was very low, sometimes below the detection limits. In some agricultural systems the input of trace elements such as As and Cd to the soil through P fertilizers application may be higher than the outputs through plant uptake and leaching; therefore the long-term use of these fertilizers may cause the trace element concentration to increase in the plow layer of agricultural soils. 相似文献
74.
Jannah Tauheed Marco Sanchez-Guerra Jane J. Lee Ligi Paul Md Omar Sharif Ibne Hasan Quazi Quamruzzaman 《Epigenetics》2017,12(6):484-491
Arsenic exposure may contribute to disease risk in humans through alterations in the epigenome. Previous studies reported that arsenic exposure is associated with changes in plasma histone concentrations. Posttranslational histone modifications have been found to differ between the brain tissue of human embryos with neural tube defects and that of controls. Our objectives were to investigate the relationships between plasma histone 3 levels, history of having an infant with myelomeningocele, biomarkers of arsenic exposure, and maternal folate deficiency. These studies took place in Bangladesh, a country with high environmental arsenic exposure through contaminated drinking water. We performed ELISA assays to investigate plasma concentration of total histone 3 (H3) and the histone modification H3K27me3. The plasma samples were collected from 85 adult women as part of a case-control study of arsenic and myelomeningocele risk in Bangladesh. We found significant associations between plasma %H3K27me3 levels and risk of myelomeningocele (P<0.05). Mothers with higher %H3K27me3 in their plasma had lower risk of having an infant with myelomeningocele (odds ratio: 0.91, 95% confidence interval: 0.84, 0.98). We also found that arsenic exposure, as estimated by arsenic concentration in toenails, was associated with lower total H3 concentrations in plasma, but only among women with folate deficiency (β = ?9.99, standard error = 3.91, P=0.02). Our results suggest that %H3K27me3 in maternal plasma differs between mothers of infants with myelomeningocele and mothers of infants without myelomeningocele, and may be a marker for myelomeningocele risk. Women with folate deficiency may be more susceptible to the epigenetic effects of environmental arsenic exposure. 相似文献
75.
Sumi D Fukushima K Miyataka H Himeno S 《Biochemical and biophysical research communications》2011,(1):48-53
Arsenic (+3 oxidation state) methyltransferase (As3MT) catalyzes the methylation of trivalent arsenic (As(III)) to monomethylarsonate (MMA(V)) and dimethylarsinic acid (DMA(V)), and plays an important role in the detoxification of arsenicals. Here, we report the identification of two splicing variants of the human As3MT gene. One splicing variant was an exon-3 skipping (Δ3) form which produced a premature stop codon, and the other was an exon-4 and -5 skipping (Δ4,5) form which produced a 31.1 kDa As3MT protein. In addition to the full-length mRNA of As3MT, Δ4,5 mRNAs were detected in HepG2, A549, HL60, K562, and HEK293 cells. The methyltransferase activity of the recombinant Δ4,5 As3MT and wild-type As3MT proteins purified from Escherichia coli was determined. Speciation analysis by HPLC–ICP-MS showed a clear peak of MMA(V) after incubation of As(III) with the wild-type As3MT protein, but not with the Δ4,5 As3MT protein. In addition, COS-7 cells transfected with Δ4,5 As3MT cDNA did not convert As(III) to MMA(V) or DMA(V). The lack of methyltransferase activity of Δ4,5 As3MT seems to be related to the deletion of an S-adenosylmethionine-binding site and a critical cysteine residue. These data suggest that the expression pattern of splicing variants of the As3MT gene may affect the capacity for arsenic methylation in cells. 相似文献
76.
77.
Rhizobium–legume symbiotic interaction is an efficient model system for soil remediation and reclamation. We earlier isolated an arsenic
(As) (2.8 mM arsenate) tolerant and symbiotically effective Rhizobium strain, VMA301 from Vigna mungo and in this study we further characterized its efficacy for arsenic removal from the soil and its nitrogen fixation capacity.
Although nodule formation is delayed in plants with As-treated composite when the inoculum was prepared without arsenic in
culture medium, whereas it attains the significant number of nodules compare to plant grown in As-free soil when the inoculum
was prepared with arsenic supplemented medium. Arsenic accumulation was higher in roots than root nodules. Nitrogenase activity
is reduced to almost 2 fold in plants with As-treated soil but not abolished. These results suggest that this strain, VMA301,
has been able to establish an effective symbiotic interaction in V. mungo in As-contaminated soil and can perform dual role of arsenic bioremediation as well as soil nitrogen improvement. 相似文献
78.
M. Azizur Rahman K. KadohashiT. Maki H. Hasegawa 《Environmental and Experimental Botany》2011,72(1):41-46
Arsenate (As(V)) transport into plant cells has been well studied. A study on rice (Oryza sativa L.) showed that arsenite is transported across the plasma membrane via glycerol transporting channels. Previous studies reported that the dimethylarsinic acid (DMAA) and monomethylarsonic acid (MMAA) uptake in duckweed (Spirodela polyrhiza L.) differed from that of As(V), and was unaffected by phosphate (H2PO4). This article reports the transport mechanisms of DMAA and MMAA in rice roots. Linear regression analysis showed that the DMAA and MMAA uptake in rice roots increased significantly (p ≤ 0.0002 and ≤0.0001 for DMAA and MMAA, respectively) with the increase of exposure time. Concentration-dependent influx of DMAA and MMAA showed that the uptake data were well described by Michaelis-Menten kinetics. The MMAA influx was higher than that of DMAA. The DMAA and MMAA uptake in rice roots were decreased significantly (p ≤ 0.0001 and ≤0.0077 for DMAA and MMAA, respectively) with the increase of glycerol concentration indicating that DMAA and MMAA were transported into rice roots using the same mechanisms of glycerol. Glycerol is transported into plant cells by aquaporins, and DMAA and MMAA are transported in a dose-dependent manner of glycerol which reveals that DMAA and MMAA are transported into rice roots through glycerol transporting channels. The DMAA and MMAA concentration in the solution did not affect the inhibition of their uptake rate by glycerol. 相似文献
79.
目的:研究三氧化二砷(As2O3)对人口腔鳞癌A431细胞生长的抑制作用,探讨其抗肿瘤的机制。方法:合成特异性靶向到肿瘤细胞表面表皮生长因子受体(EGFR)的近红外荧光分子对比剂EGF-Cy5.5,验证试剂合成的靶向特异性。口腔鳞状细胞癌A431细胞系暴露于浓度分别为0μM,0.5μM,2.5μM和5.0μM的三氧化二砷溶液中0,24 h,48 h和72 h。共聚焦显微镜、流式细胞仪及免疫组化证实EGFR的表达水平,上述实验均测量三次,结果取平均值。结果:EGF-Cy5.5靶向荧光对比剂的标记率为68%~70%。对比对照组,越高浓度的三氧化二砷处理的肿瘤细胞其获得的细胞荧光信号强度越小,这与药物浓度越高细胞表面表达EGFR的量越少相一致。流式细胞仪显示,在72小时,作用于细胞的三氧化二砷药物浓度分别为0.5μM,2.5μM,和5.0μM,其相对应获得的细胞EGFR表达量分别为57.28±3.2%(P〈0.05),29.91±2.2%(P〈0.01)和10.73±2.4%(P〈0.01),明显低于对照组的细胞EGFR表达量74.42±1.8%,(P〈0.05)。结论:本研究应用近红外荧光分子成像的方法体外检测口腔鳞状细胞癌A431的EGFR表达水平,实验证明三氧化二砷对其EGFR具有明显的抑制作用,且抑制作用具有时间-剂量依赖性。 相似文献
80.