全文获取类型
收费全文 | 1386篇 |
免费 | 135篇 |
国内免费 | 233篇 |
出版年
2024年 | 4篇 |
2023年 | 25篇 |
2022年 | 21篇 |
2021年 | 38篇 |
2020年 | 50篇 |
2019年 | 57篇 |
2018年 | 57篇 |
2017年 | 66篇 |
2016年 | 76篇 |
2015年 | 64篇 |
2014年 | 61篇 |
2013年 | 104篇 |
2012年 | 46篇 |
2011年 | 71篇 |
2010年 | 42篇 |
2009年 | 116篇 |
2008年 | 100篇 |
2007年 | 98篇 |
2006年 | 92篇 |
2005年 | 61篇 |
2004年 | 62篇 |
2003年 | 49篇 |
2002年 | 41篇 |
2001年 | 41篇 |
2000年 | 30篇 |
1999年 | 26篇 |
1998年 | 23篇 |
1997年 | 33篇 |
1996年 | 25篇 |
1995年 | 25篇 |
1994年 | 24篇 |
1993年 | 14篇 |
1992年 | 14篇 |
1991年 | 17篇 |
1990年 | 10篇 |
1989年 | 13篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 12篇 |
1985年 | 12篇 |
1984年 | 5篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 3篇 |
排序方式: 共有1754条查询结果,搜索用时 31 毫秒
81.
Seasonal variation in uptake and regeneration of ammonium and nitrate in a coastal lagoon was studied using 15N incorporation in particulate matter and by measuring changes in particulate nitrogen. Uptake and regeneration rates were two orders of magnitude lower in winter than in summer. Summer uptake values were 2.8 and 2.2 mol N.l–1.d–1 for ammonium and nitrate, respectively. Regeneration rates were 2.9 and 2.1 mol N.l–1.d–1 for ammonium and nitrate respectively. Regeneration/uptake ratios were often below one, indicating that water column processes were not sufficient to satisfy the phytoplankton nitrogen demand. This implies a role of other sources of nitrogen, such as macrofauna (oysters and epibionts) and sediment. Phytoplankton was well adapted to the seasonal variations in resources, with mixotrophic dinoflagellates dominant in winter, and fast growing diatoms in summer. In winter and spring, ammonium was clearly preferred to nitrate as a nitrogen source, but nitrate was an important nitrogen source in summer because of high nitrification rates. Despite low nutrient levels, the high rates of nitrogen regeneration in summer as well as the simultaneous uptake of nitrate and ammonium allow high phytoplankton growth rates which in turn enable high oyster production. 相似文献
82.
The urban heat island in a small city in coastal Portugal 总被引:3,自引:0,他引:3
This project arose from the need to study the phenomenon of the urban heat island, since only by recognising this phenomenon
can we moderate it to improve the human and urban environments. Not only big cities develop urban heat islands. This study
detected the presence and recorded the characteristics of an urban heat island in the small coastal city of Aveiro, Portugal.
The study was developed through the scheduled measurements of air temperature and the analysis of the geographical, meteorological
and urban conditions. The form and intensity of Aveiro’s heat island are a response to the interaction of three principal
factors: the urban morphology (the hottest zones in the city are those with the tallest and the highest density of buildings,
without green spaces and with intense generation of heat from traffic, commerce and services); the meteorological conditions
(the intensity of the island is at its maximum when the sky is totally clear and there is no wind, and at its minimum in those
situations when there is atmospheric instability, such as wind, cloud and precipitation); and the proximity of the coastal
lagoon (which borders the city to the west and northwest and moderates seasonal temperatures. The urban heat island influences
the comfort and health of its inhabitants, thus urban planning is very important in the moderation and prevention of this
phenomenon.
Received: 7 July 1999 / Revised: 24 April 2000 / Accepted: 26 April 2000 相似文献
83.
Eriophorum vaginatum and E. angustifolium are dominant arctic sedges of the well-drained tussock tundra and the permanently flooded wet-sedge tundra, respectively. We determined diurnal courses of gas exchange and water relations of the two species in their natural habitat and compared their responses to changes in light, air temperature, and humidity. Mean photosynthetic response to light was similar between E. angustifolium and E. vaginatum and carbon gain in both species was light limited during most of the growing season. On sunny and dry days, both species closed stomata in response to high leaf-to-air vapor pressure deficits. Even though E. angustifolium was growing in standing water, it exhibited a tighter control of transpirational water loss and had lower hydraulic conductivity in the soil-root-shoot pathway than E. vaginatum. The different response pattern between the two species is discussed in the context of differences in habitat conditions. 相似文献
84.
Despite geographical isolation and widespread phenotypic polymorphism, previous population genetic studies of Arctic charr, Salvelinus alpinus , have detected low levels of intra- and interpopulation variation. In this study, two approaches were used to test the generality of low genetic diversity among 15 Arctic charr populations from three major drainages of the central Alpine region of Europe. First, a representative subsample of each drainage was screened by PCR–RFLP analysis of mtDNA using 31 restriction enzymes. All individuals but one shared an identical haplotype. In contrast, microsatellite DNA variation revealed high levels of genetic diversity within and among populations. The number of alleles per locus ranged from six to 49, resulting in an overall expected heterozygosity from 0.72 ± 0.09 to 0.87 ± 0.04 depending on the locus. Despite evidence for fish transfers among Alpine charr populations over centuries, genetic diversity was substantially structured, as revealed by hierarchical Φ statistics. Eighteen per cent of total genetic variance was apportioned to substructuring among Rhône, Rhine, and Danube river systems, whereas 19% was due to partitioning among populations within each drainage. Cluster analyses corroborated these results by drainage-specific grouping of nonstocked populations, but also revealed damaging effects of stocking practices in others. However, these results suggest that long-term stocking practices did not generally alter natural genetic partitioning, and stress the importance of considering genetic diversity of Arctic charr in the Alpine region for sound management. The results also refute the general view of Arctic charr being a genetically depauperate species and show the potential usefulness of microsatellite DNAs in addressing evolutionary and conservation issues in this species. 相似文献
85.
M. Holmstrup L. Sømme 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(3):197-203
Specimens of the Arctic Collembolon Onychiurus arcticus were exposed to desiccation at several subzero temperatures over ice and at 0.5 °C over NaCl solutions. The effects of desiccation
on water content (WC), body fluid melting point (MP), supercooling point (SCP) and survival were studied at several acclimation
temperatures and relative humidities. Exposure to temperatures down to −19.5 °C caused a substantial and increasing dehydration.
At the lowest exposure temperature unfrozen individuals lost 91.6% of the WC at full hydration but more than 80% of the individuals
survived when rehydrated. Exposure at 0.5 °C to decreasing relative humidities (RH) from 100% to 91.3% caused increasing dehydration
and increasing mortality. Survival of equally dehydrated individuals was higher at subzero temperatures than at 0.5 °C. Concurrent
with the decline in WC a lowering of the MP was observed. Animals exposed to −3 °C and −6 °C over ice for 31 days had a MP
of −3.8 and < −7.5 °C, respectively. Specimens from a laboratory culture had a mean SCP of −6.1 °C, and acclimation at 0 or
−3 °C had little effect on SCPs. Exposure at −8.2 °C over ice for 8 days, however, caused the mean SCP to decline to −21.8 °C
due to the severe dehydration of these individuals. Dehydration at 0.5 °C in 95.1 and 93.3% RH also caused a decline in SCPs
to about −18 °C. Individuals that had been acclimated over ice at −12.4 °C or at lower temperatures apparently did not freeze
at all when cooled to −30 °C, probably because all freezeable water had been lost. These results show that O. arcticus will inevitably undergo dehydration when exposed to subzero temperatures in its natural frozen habitat. Consequently, the
MP and SCP of the Collembola are substantially lowered and in this way freezing is avoided. The increased cold hardiness by
dehydration is similar to the protective dehydration mechanism described in earthworm cocoons and Arctic enchytraeids.
Accepted: 5 January 1998 相似文献
86.
Distribution,diversity and drivers of blood-borne parasite co-infections in Alaskan bird populations
Khouanchy S. Oakgrove Ryan J. Harrigan Claire Loiseau Sue Guers Bruce Seppi Ravinder N.M. Sehgal 《International journal for parasitology》2014
Avian species are commonly infected by multiple parasites, however few studies have investigated the environmental determinants of the prevalence of co-infection over a large scale. Here we believe that we report the first, detailed ecological study of the prevalence, diversity and co-infections of four avian blood-borne parasite genera: Plasmodium spp., Haemoproteus spp., Leucocytozoon spp. and Trypanosoma spp. We collected blood samples from 47 resident and migratory bird species across a latitudinal gradient in Alaska. From the patterns observed at collection sites, random forest models were used to provide evidence of associations between bioclimatic conditions and the prevalence of parasite co-infection distribution. Molecular screening revealed a higher prevalence of haematozoa (53%) in Alaska than previously reported. Leucocytozoons had the highest diversity, prevalence and prevalence of co-infection. Leucocytozoon prevalence (35%) positively correlated with Trypanosoma prevalence (11%), negatively correlated with Haemoproteus prevalence (14%) and had no correlation with Plasmodium prevalence (7%). We found temperature, precipitation and tree cover to be the primary environmental drivers that show a relationship with the prevalence of co-infection. The results provide insight into the impacts of bioclimatic drivers on parasite ecology and intra-host interactions, and have implications for the study of infectious diseases in rapidly changing environments. 相似文献
87.
Sønstebø JH Gielly L Brysting AK Elven R Edwards M Haile J Willerslev E Coissac E Rioux D Sannier J Taberlet P Brochmann C 《Molecular ecology resources》2010,10(6):1009-1018
Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13–158 bp) and variable region with highly conserved flanking sequences. For taxonomic reference, a whole trnL intron sequence database was constructed from recently collected material of 842 species, representing all widespread and/or ecologically important taxa of the species-poor arctic flora. The P6 loop alone allowed identification of all families, most genera (>75%) and one-third of the species, thus providing much higher taxonomic resolution than pollen records. The suitability of the P6 loop for analysis of samples containing degraded ancient DNA from a mixture of species is demonstrated by high-throughput parallel pyrosequencing of permafrost-preserved DNA and reconstruction of two plant communities from the last glacial period. Our approach opens new possibilities for DNA-based assessment of ancient as well as modern biodiversity of many groups of organisms using environmental samples. 相似文献
88.
华北驼绒藜种群数量动态与生殖特性的相关性 总被引:1,自引:0,他引:1
采用立木级法研究华北驼绒藜种群的大小级结构,利用各龄级的存活数编制种群静态生命表和存活曲线,构建时间序列预测图对种群数量动态过程进行预测,利用生活史特征观察结果分析其生殖对策,探讨了生殖特性与种群动态的关系。结果表明:华北驼绒藜种群幼龄和老龄级个体数较少,中龄级个体数量较多,呈稳定或衰退趋势;种群生命表和存活曲线的分析表明,尽管生境条件不同,但存活曲线基本接近DeeveyⅢ型;种群数量的时间序列预测表明,华北驼绒藜种群会随时间推移,呈现老龄级株数先增加后减少的趋势,种群稳定性长期维持困难;生活史特征表明,华北驼绒藜在长期进化过程中形成了兼具r-对策和k-对策的双重特性来维持种群的相对稳定。良好的生殖适应性是物种发展的关键,华北驼绒藜种群年龄结构、生命表、存活曲线以及时间序列预测图反映出其种群数量动态与生殖特性具有密切的相关性,传粉系统效率低、胚胎发育过程营养失调、种子向幼苗的转化率低以及种群更新对母株的依赖性强等生殖生物学特性与种群动态相吻合;生境恶劣和人为干扰也成为其种群发展的制约因素。 相似文献
89.
Lawrence J. Weider Anders Hobk John K. Colbourne Teresa J. Crease France Dufresne Paul D. N. Hebert 《Evolution; international journal of organic evolution》1999,53(3):777-792
Pleistocene glacial cycles undoubtedly altered the evolutionary trajectories of many taxa, yet few studies have examined the impact of such events on genetic differentiation and phylogeography at large geographic scales. Here we present the results of a circumarctic survey of mitochondrial DNA diversity in members of the Daphnia pulex complex. The analysis involved the survey of restriction site polymorphisms in a 2100-bp fragment of the NADH-4 (ND4) and NADH-5 (ND5) genes for 276 populations representing the two major groups (tenebrosa and pulicaria) in this complex across their Holarctic range. A comparison of the distribution patterns for seven clades in this complex revealed very clear phylogeographic structuring. Most notably, pulicaria group lineages were restricted primarily to the Nearctic, with some colonization of formerly glaciated portions of northern Europe. This group was not detected from vast expanses of northern Eurasia, including the Beringian glacial refuge. In contrast, tenebrosa group haplotypes showed considerable intercontinental divergence between Eurasian and North American lineages, but were absent from Greenland and Iceland, as well as the Canadian arctic archipelago. Dispersal in Eurasia was primarily in a westerly direction from Beringia, whereas dispersal in the Nearctic followed proglacial drainage patterns. Long-distance dispersal of certain lineages was observed in both groups, and variation in haplotype richness and nucleotide diversity allowed us to make inferences about the positioning of putative glacial refugia. Overall, the phylogeographic pattern of diversification in this arctic complex is characterized by the apparently unique postglacial histories for each clade, indicating that even closely allied taxa can respond independently to the allopatric effects of glacial cycles. This is in sharp contrast to other phylogeographic studies of species assemblages from more southern (unglaciated) latitudes, which are often characterized by concordant patterns. 相似文献
90.
A. S. Savvichev I. I. Rusanov N. V. Pimenov E. E. Zakharova E. F. Veslopolova A. Yu. Lein K. Crane M. V. Ivanov 《Microbiology》2007,76(5):603-613
The research performed in August 2004 within the framework of the Russian-American Long-term Census of the Arctic (RUSALCA) resulted in the first data concerning the rates of the key microbial processes in the water column and bottom sediments of the Bering strait and the Chukchi Sea. The total bacterial counts in the water column varied from 30 × 103 cells ml?1 in the northern and eastern parts to 245 × 103 cells ml?1 in the southern part. The methane content in the water column of the Chukchi sea varied from 8 nmol CH4l?1 in the eastern part of the sea to 31 nmol CH4l?1 in the northern part of the Herald Canyon. Microbial activity occurred in the upper 0–3 cm of the bottom sediments; the methane formation rate varied from 0.25 to 16 nmol CH4dm?3 day?1. The rates of methane oxidation varied from 1.61 to 14.7 nmol CH4dm?3 day?1. The rates of sulfate reduction varied from 1.35 to 16.2 μmol SO 4 2? dm?1 day?1. The rate of methane formation in the sediments increased with depth, while sulfate reduction rates decreased (less than 1 μmol SO 4 2? dm?3 day?1). These high concentrations of biogenic elements and high rates of microbial processes in the upper sediment layers suggest a specific type of trophic chain in the Chukchi Sea. The approximate calculated balance of methane emission from the water column into the atmosphere is from 5.4 to 57.3 μmol CH4m?2 day?1. 相似文献