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131.
高羊肚菌菌丝球液体培养的研究 总被引:4,自引:0,他引:4
张松 《中国野生植物资源》1996,(1):25-26
本文探讨了高羊肚菌菌丝球在不同液体培养基中的生长情况。结果表明,在黄豆芽玉米玢培养基中菌丝球最多,大小均匀,菌丝生长量最大,在麦麦夫蛋白胨培养基上菌丝的糖含量最高。 相似文献
132.
太子参中环肽Pseudostellarin B含量HPCE测定及其指纹图谱研究 总被引:1,自引:0,他引:1
建立了测定太子参中环肽Pseudostellarin B含量的高效毛细管电泳(HPCE)分析方法,对10种不同产地太子参中环肽Pseudostellarin B的含量进行了测定。HPCE工作条件:采用未涂层熔融石英毛细管(内径75μm,有效长度50cm),分离电压为15kV,柱温20℃,二极管阵列检测器(DAD)检测波长为203nm,缓冲液为20mmol/L硼砂(pH=9.3)溶液。在选定的工作条件下,环肽Pseudostellarin B浓度与其响应信号值之间具有较好的线性相关性(Y=0.6357X 2.546,R=0.9985),加标回收率在93.8%~105.6%之间。在此基础上进行了太子参HPCE指纹图谱研究,采用中药指纹图谱相似度计算软件对不同太子参样品的HPCE指纹图谱进行相似度计算,以系统生成的对照指纹图谱为对照模板,10份样品中有8份的相似度在0.90以上,说明该方法可用于太子参质量控制。 相似文献
133.
通过实验室模拟初春温度(17℃)条件,研究了沉水植物马来眼子菜(Potamogeton malaianus Miq.)分解过程对其有机碳、总氮和总磷的释放,水体和底泥中有机碳、总氮和总磷含量以及水体中铵态氮和硝态氮含量的影响,同时对水体N2和N2O的释放状况进行了分析。结果表明:在70 d的实验周期内,马来眼子菜分解迅速,至实验结束时植株失重率达到86.92%,植株中有机碳量、总氮量和总磷量分别较实验初始时降低了88.51%、88.93%和86.63%;底泥中有机碳、总氮和总磷含量分别较实验初始时增加了5.56%、17.06%和2.17%。在马来眼子菜分解过程中,水体中的溶解氧含量和氧化还原电位均较对照明显降低,并呈现在实验初期迅速下降然后逐渐增加并趋于稳定的趋势;水体中的有机碳、总氮、总磷以及铵态氮含量均较对照大幅度增加,并呈现在实验初期迅速上升然后逐渐下降并趋于稳定的趋势;而水体中的硝态氮含量则呈先下降后上升再下降的趋势。水体中N2和N2O释放通量较对照明显增加,且在实验中期N2和N2O释放通量达到峰值。研究结果表明:随时间推移,马来眼子菜向水体释放的磷大部分沉积到底泥中,而氮则部分沉积到底泥中、部分以气体形式(N2和N2O)逸出水体。 相似文献
134.
《Bioscience, biotechnology, and biochemistry》2013,77(2):542-545
The carbohydrate specificity of three novel lectins, Boletopsis leucomelas lectin (BLL), Aralia cordate lectin (ACL), and Wasabia japonica lectin (WJL), was examined by frontal affinity chromatography using a panel of fluorescently labeled 47 oligosaccharides. The results indicate that BLL recognizes an agalacto structure of the biantennary chain and its bisecting structure. ACL showed strong affinity for triantennary oligosaccharides, but no affinity for tetraantennary structure. WJL showed no appreciable affinity for any of the 47 glycans examined. These lectins with a unique affinity specificity might be useful for examining alterations in the glycan structures of the glycoconjugates in association with development and various diseases. 相似文献
135.
Fei Xie Dan-Dan Xia Hao-Jie Duan Yue Sun Zhi-Feng Zi Dai-Yu Wan Hao Zhou Zhong-Tao Ding 《化学与生物多样性》2023,20(8):e202300566
Two new decarestrictine analogs decarestrictine P and penicitone, together with eight known homologous compounds were isolated from the soil fungus from the rhizosphere of Penicillium sp. YUD18003 related to Gastrodia elata. Their different structures include a decanolides decartestridine P and a long-chain polyhydroxyketone penicitone. The structures of new compounds were determined by nuclear magnetic resonance (NMR) spectroscopic analysis and high resolution electrospray ionization mass spectrometry (HR-ESI-MS), while their absolute configurations were determined by spectroscopic methods, DP4+ probability analysis, modified Snatzke's method and electron circular dichroism (ECD) calculations. All compounds were evaluated for antimicrobial activities. 相似文献
136.
137.
138.
全国栽培太子参生态适宜性区划分析 总被引:5,自引:0,他引:5
采用Arc GIS地理信息系统软件的空间分析方法和SPSS统计软件的统计分析方法,分析了全国太子参潜在适宜种植地理分布、适宜生境及化学成分与环境因子的相关性。结果显示:采用聚类分析从46个样地中筛选出34个多糖含量较高的样地。所建模型经ROC曲线验证,预测效果非常好(AUC0.9)。最干月降水量(贡献率68.4%)、土壤类型(9.7%)、最暖季平均温(6.0%)、等温性(4.0%)是影响太子参种植分布最重要的环境因子,其最适宜生境范围为:最干月降水量20—60mm;最暖季平均温21.5—30.5℃;等温性18%—35%;土壤类型为不饱和薄层土、饱和粘磬土中的黄棕壤、黄壤、黄红壤。相关性分析显示,多糖和生境适宜度均与最湿季降水量、最暖季降水量、最暖季平均温、最干月降水量及年平均气温无显著相关性。太子参最适宜区主要分布在长江中下游区域,主要有贵州中部,重庆与湖南、湖北接壤处,河南南部,安徽西部,江苏中部,福建东北部以及浙江北部和东南部区域。因此,明确全国太子参潜在的适宜种植区可为太子参种植基地的选取和合理布局提供参考。 相似文献
139.
Kazuo Sakamoto Kumiko Iida Kimiko Sawamura Kyoko Hajiro Yoshihisa Asada Takafumi Yoshikawa Tsutomu Furuya 《Plant Cell, Tissue and Organ Culture》1994,36(1):21-26
High anthocyanin-producing cell lines, which were grown in a dark or in a light-dark regime, were selected from callus cultures initiated from stem and leaf tissues of Aralia cordata Thunb. by small-cell-aggregate selection. To verify the optimum culture conditions for anthocyanin production, cells were tested by changing the various basal media, sucrose concentration and nitrogen source and concentration. Good growth was obtained in the dark on Linsmaier-Skoog's basal medium containing 1.0 mg l-1 2,4-d and 0.1 mg l-1 kinetin, 2% (w/v) sucrose and full strength of nitrogen concentration. However, the highest anthocyanin yield (10.3% dry wt) was obtained in the dark on B5 medium containing 1.0 mg l-1 2,4-d and 0.1 mg l-1 kinetin. Our results suggested that it has became feasible to find the most effective conditions for cell growth and anthocyanin production by optimizations of the nitrogen concentration and the ratio of NH4
+ to NO3
- in the medium.Abbreviations B5
Gamborg (Gamborg et al. 1968)
- 2,4-d
2,4-dichlorophenoxyacetic acid
- LS
Linsmaier and Skoog (Linsmaier & Skoog 1965)
- MS
Murashige and Skoog (Murashige & Skoog 1962)
- NN
Nitsch and Nitsch (Nitsch & Nitsch 1967)
- WH
White (White 1963)
This paper is part 81 in the series Studies on Plant Tissue Cultures. For Part 80 see Furuya T, Sakamoto K, Iida K, Asada Y, Yoshikawa T, Sakai S & Aimi N (1992) Phytochemistry 31: 3065–3068. 相似文献
140.
Pollen of ten species of Panax and six species of Aralia was examined in light microscopy and scanning and transmission electron microscopy. Grains of both genera have similar complex apertures, short columellae, and overlapping tectal sculptures, suggesting a close relationship. Most species of Panax have pollen characterized by striato-reticulate tecta, short columellae, thick foot layers, costa ectocolpi, and lalongate endoapertures. The eastern North American P. trifolius, commonly known as the dwarf ginseng, has a distinctive pollen morphology and exine structure, supporting the hypothesis of its phylogenetically isolated position. Pollen of the eastern Asian P. ginseng (ginseng) can be distinguished from the eastern North American P. quinquefolius (American ginseng) by differences in ultrastructure. The monophyly of the three medicinally important species, P. ginseng, P. notoginseng, and P. quinquefolius, suggested by triterpenoid data, is not supported by pollen data. The results of the pollen study are generally congruent with those from the sequences of nuclear ribosomal DNA. 相似文献