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以红掌盆栽品种‘Avo-Gloria’为试材,以MS+0.2mg·L^-12,4-D为基本培养基,分别在添加1~10mg·L^-16-BA的10种脱分化培养基上,诱导其叶柄外植体产生愈伤组织;再以MS+2mg·L^-16-BA+0.2mg·L^-1 NAA为分化培养基诱导分化不定芽;以MS+0.2mg·L^-1 NAA为生根培养基,从不定芽获得再生植株。结果显示:(1)在MS+0.2mg·L^-12,4-D+8~10mg·L^-16-BA的3种脱分化培养基上可产生9%~10%的绿色、质地较硬的愈伤组织;(2)愈伤组织在MS+2mg·L^-16-BA+0.2mg·L^-1 NAA的分化培养基上,经6~8次继代培养,可获得3%~7%的不定芽,并可生根长成再生植株;(3)再生植株定植3个月后,有3%~7%植株出现红叶变异,此红叶可终生表现为红色。 相似文献
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Efficacy of hot water drenches of Anthurium andraeanum plants against the burrowing nematode Radopholus similis and plant thermotolerance 总被引:2,自引:0,他引:2
Hot‐water drench treatments were evaluated for disinfesting roots of potted anthurium Anthurium andraeanum of the burrowing nematode Radopholus similis. A continuous drench of roots and media in pots with 50°C water for 5 to 20 min eliminated or reduced nematode populations to < 1 g?1 of dry root. A second hot water drench, 2 months after the first drenching, tended to increase the efficacy of the heat treatment. A few survivors persisted in the roots and/or stems of a few plants 2 to 4 months after heat treatment. Non‐treatment of the shoots and possible migration from stem to root tissues are probable causes of nematode survival. Drenching potting media and roots in pot were as effective against R. similis in the roots as hot water dipping bare‐rooted plants. Plant response to hot‐water drenching varied among cultivars, but most exhibited tolerance to the heat treatments. Visual inspection of the plants showed little difference between treated and untreated plants in the heat tolerant cultivars. However, all heat‐treated ‘Marian Seefurth’ plants, especially hot water‐dipped bare‐rooted plants, appeared to suffer some degree of growth reduction as measured by lower root and stem dry weights when compared to untreated plants 3 months after treatment. Conditioning anthurium plants with hot water or hot air prior to hot water drenching did not benefit plant quality when compared to unconditioned, heat‐treated plants. 相似文献
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钙和硼对蓝猪耳花粉萌发及花粉管生长的影响 总被引:31,自引:1,他引:31
研究了钙(Ca^2 )和硼(H3BO3)对蓝猪耳花粉萌发和花粉管生长的影响。结果表明:(1)在一定范围内Ca^2 几乎不影响花粉萌发频率,而主要影响花粉萌发速度和花粉管生长速度;低Ca^2 不利于花粉管生长,而高Ca^2 抑制花粉萌发速度和花粉管生长;在稍高于最适Ca^2 浓度的条件下,花粉管生长早期呈现波浪形。(2)硼明显影响花粉萌发频率及花粉管形态;花粉管生长必需硼,但不同浓度的硼对花粉管生长速度影响不明显;在高浓度硼条件下,较长时间内花粉管均呈现出波浪形。(3)Cooled-CCD动态跟踪观察进一步证实Ca^2 影响花粉管生长速度,而硼则不明显。 相似文献
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用安祖花叶片作为外植体,诱导产生致密愈伤组织,将安祖花致密愈伤组织切成规则小块,用(MS+0.1 mg/L NAA)培养基预培养半个月,诱导根的发生。再用继代培养基包埋培养物进行固定化培养,经1月后筛选具不定根的培养物,采用再包埋方法制成人工种子。当这种人工种子播于有菌土壤时可得到63%的成活率(转株率)。我们认为致密愈伤组织中不定芽和愈伤组织间发生的是生物学接触,不定芽的初始生长由愈伤组织通过生物学途径提供营养,从而大大提高了人工种子的活力。用含不定芽的致密愈伤组织作为培养物,对于人工种子的机械化生产和田间应用具有参考价值。 相似文献
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We studied the reproductive biology of three sympatric Araceae species, Anthurium sagittatum, A. thrinax and Spathiphyllum humboldtii in French Guiana. The plants flowered simultaneously and were visited by scent‐collecting male euglossine bees, which were apparently their major pollinators. In total, each species was visited by 3–7 euglossine species, and 2–3 euglossine species accounted for at least 80% of all flower visits, with visits being plant species‐specific. Floral scent consisted of 6–10 main compounds, which made up 76–94% of the total amount of volatiles and were specific in these high amounts to each plant species. We suggest that the different floral scents lead to clear separation of the main pollinating euglossine species, providing a directed and efficient intraspecific pollen flow that results in high reproductive success. Since the simple floral (inflorescence) morphology of the studied plants does not support any morphological mechanisms to exclude visitors, as for example in euglossine‐pollinated perfume orchids, floral scent might be of major importance for the reproductive isolation and sympatric occurrence of these plants. 相似文献
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采用低温半致死温度( LT50)评价低温条件下10个红掌( Anthurium andraeanum Lind.)品种的抗寒性;并在测定高温(39℃)处理48 h后各品种叶片生理指标的基础上,对这些指标的耐热系数和相关系数进行分析,采用主成分分析和隶属函数法综合评价了10个红掌品种的耐热性。结果表明:经过-1℃、-3℃、-5℃、-7℃和-9℃的低温处理,10个品种的叶片相对电导率( REC)均逐渐增大,其中,品种‘阿拉巴马’(‘Alabama’)的REC值呈“缓慢升高—迅速升高”的趋势,其他品种的REC值均呈“缓慢升高—迅速升高—平稳升高”的趋势;依据Logistic方程获得的各品种的LT50值,10个红掌品种的抗寒性由强至弱依次排序为‘阿拉巴马’,‘皇冠’(‘Royal champion’),‘马都拉’(‘Madural ’),‘甜冠军’(‘Sweet champion ’),‘特伦萨’(‘Turenza ’),‘阿瓦托’(‘Avento ’)、‘阿瑞博’(‘Arebo’)和‘粉冠军’(‘Pink champion’),‘白冠军’(‘White champion’),‘骄阳’(‘Sierra’)。高温处理48 h对各品种叶片的生理指标均有一定影响,其中,REC值和CAT活性明显高于对照(室温)、MDA含量高于或等于对照,它们的耐热系数均大于100%;相对含水量、叶绿素和类胡萝卜素及脯氨酸含量均低于对照,它们的耐热系数均小于100%;多数品种叶片的SOD和POD活性及可溶性蛋白质含量( SPC)低于对照,耐热系数也小于100%;仅品种‘阿拉巴马’叶片的SOD和POD活性及SPC值略高于对照,耐热系数大于100%;各指标的耐热系数间均存在一定的相关性。主成分分析结果显示:前3个主成分的累计贡献率达到91.50%,其中,第1主成分定义为酶活性和渗透调节物质含量,第2主成分定义为光合色素含量,第3主成分定义为膜透性。根据耐热性综合评价值,10个红掌品种耐热性由强至弱依次排序为‘阿拉巴马’、‘皇冠’、‘阿瓦托’、‘阿瑞博’、‘马都拉’、‘骄阳’、‘特伦萨’、‘粉冠军’、‘甜冠军’、‘白冠军’。研究结果表明:品种‘阿拉巴马’的抗寒性和耐热性均较强,可以在不同气候区推广种植。 相似文献
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《Biotechnic & histochemistry》2013,88(4):205-207
A method is presented by which whole embryos, immature animals, parts of organs, or even thick sections, of vertebrate material are very slowly dehydrated, thoroughly cleared, and then dried to show the topography of complicated structures.A representative photomicrograph is included to show the result obtained. 相似文献
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