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81.
The Origin of the Oxidative Burst in Plants 总被引:18,自引:0,他引:18
G. Paul Bolwell Vernon S. Butt Dewi R. Davies Alfred Zimmerlin 《Free radical research》1995,23(6):517-532
A large number of publications recently have drawn strong analogies between the production of active oxygen species in plant cells and the “oxidative burst” of the phagocyte, even to the point of constructing elaborate models involving receptor mediated G-protein activation of a plasmalemma NADPH oxidase in plant cells. However there are potentially other active oxygen species generating systems at the plant cell surface. The present work examines these alternatives with particular emphasis on the rapid production of active oxygen species, in common with a number of other systems, by suspension-cultured cells of French bean on exposure to an elicitor preparation from the fungal pathogen Colletotrichum lindemuthianum. The cells show a rapid increase in oxygen uptake which is followed shortly afterwards by the appearance of a burst of these active oxygen species, as measured by a luminescence assay, which is probably all accounted for by hydrogen peroxide. An essential factor in this production of H2O2 appears to be a transient alkalinization of the apoplast where the pH rises to 7.0-7.2. Dissipation of this pH change with a number of treatments, including ionophores and strong buffers, substantially inhibits the oxidative burst. Little evidence was found for enhanced activation of a membrane-bound NADPH oxidase. However the production of H2O2 under alkaline conditions can be modelled in vitro with a number of peroxidases, one of which, an Mr 46,000 wall-bound cationic peroxidase, is able to sustain H2O2 production at neutral pH unlike the other peroxidases which only show low levels of this reaction under such conditions and have pH optima at values greater than 8.0. On the basis of such comparative pH profiles between the cells and the purified peroxidase and further inhibition studies a direct production of H2O2 from the wall peroxidase in French bean cells is proposed. These experiments may mimic some of the responses to plant pathogens, particularly the hypersensitive response, which is an important feature of resistance. A cell wall peroxidase-origin for the oxidative burst is clearly different from a model consisting of receptor activation of a plasmaiemma-localised NADPH oxidase generating superoxide. An alternative simple and rapid mechanism thus exists for the generation of H2O2 which does not require such multiple proteinaceous components. 相似文献
82.
Abstract. Altitudinal and latitudinal distribution limits of trees are mainly controlled by temperature. Therefore climate warming is expected to induce upslope or poleward migrations. In the Swiss Central Alps, summers in the period 1982-1991 were on average 0.8 °C warmer than those of the period 30 yr before. We investigated whether populations of conifers at the montane Pinus sylvestris-Pinus cembra ecocline exhibit demographic trends in response to that warming. We found no evidence for this. Young seedlings of Pinus sylvestris, the species which is expected to expand its range upward in a warmer climate, were virtually absent from all sites, whereas large fractions of Pinus cembra populations were observed in the seedling and juvenile categories even below the present lower distribution limit of adult trees. This suggests that there are no major altitudinal shifts in response to the recent sequence of warmer summers. Germination and seedling survival trials with Pinus sylvestris suggest that temperature per se would not exclude this species even from establishing at the current treeline in the Swiss Central Alps. Similar results were found at the polar treeline. Phytotron tests of seedling survival showed much less drought resistance in Pinus sylvestris than in Pinus cembra which is in contrast to their phytogeographic distributions. Thus, the montane pine ecocline in the Swiss Central Alps seems to be stabilized by species interactions and may not be directly responsive to moderate climatic change, which needs to be taken into account in predictive attempts. 相似文献
83.
Effect of season and photoperiod on the follicle-stimulating hormone receptors in a subtropical bird
Annual changes in and photoperiodic influence oh the weight of gonads, a parameter of gonadal activity, are much smaller in
female birds than in males. Effect of season and photoperiod on the follicle-stimulating hormone receptors in the testis or
ovary was studied using a subtropical weaver finch. The number of follicle-stimulating hormone binding sites per unit testicular
weight showed a peak in the non-breeding phase; while the total number of binding sites per two testes was maximal in the
breeding phase and minimal in the regressive phase. In contrast, seasonal changes in follicle-stimulating hormone binding
sites in the ovary were less marked. Exposure to short-day during the breeding phase induced marked decreases in the numbers
of binding sites per unit testicular weight and per two testes. These numbers markedly increased after transfer to long-day
during the non-breeding phase. However, there was no significant effect of short-day or long-day exposure on follicle-stimulating
hormone binding sites in the ovary. These results suggest that photoperiod is an effective environmental factor in the regulation
of follicle-stimulating hormone receptors in the testis and the effect is manifested by pronounced changes in the testicular
weight during annual breeding cycle. 相似文献
84.
In this paper we analyze a model for the HIV-infection transmission in a male homosexual population. In the model we consider two types of infected individuals. Those that are infected but do not know their serological status and/or are not under any sort of clinical /therapeutical treatment, and those who are. The two groups of infectives differ in their incubation time, contact rate with susceptible individuals, and probability of disease transmission. The aim of this article is to study the roles played by detection and changes in sexual behavior in the incidence and prevalence of HIV. The analytical results show that there exists a unique endemic equilibrium which is globally asymptotically stable under a range of parameter values whenever a detection /treatment rate and an indirect measure of the level of infection risk are sufficiently large. However, any level of detection/ treatment rate coupled with a decrease of the transmission probability lowers the incidence rate and prevalence level in the population. In general, only significant reductions in the transmission probability (achieved through, for example, the adoption of safe sexual practices) can contain effectively the spread of the disease. 相似文献
85.
Mark D. Schwartz 《International journal of biometeorology》1994,38(1):18-22
The centuries-old practice of recording plant and animal events that take place at specific times each year (phenology) should play an important role in monitoring mid-latitude global changes. At least three problems related to the detection of biosphere changes could be investigated using this information. Firstly, the technique can be generalized from the local to global scale. Secondly, an integrated approach could be developed to represent biome diversity effectively. Lastly, physical mechanisms responsible for the events can be deduced in order to incorporate the phenological information into global-scale models, and detect changes in related environmental factors. With these goals in mind, regional phenological data collection networks were initiated in eastern North America during the early 1960s, using cloned lilacs and several species of honeysuckle. This paper reviews research projects which address the problems outlined above, using first leaf data (associated with spring green-up or green wave in mid-latitudes) gathered from these networks. The results of such studies in North America have demonstrated the potential of phenology as an efficient monitor of global change throughout mid-latitude regions. Future research efforts will concentrate on the development of a coordinated strategy to link phenological information from satellites, indicator plants (such as the lilac), and representative species from each biome. 相似文献
86.
At elevated atmospheric CO2 concentrations ([CO2]a), photosynthetic capacity (Amax) and root fraction (ηR, the ratio of root to plant dry mass) increased in some studies and decreased in others. Here, we have explored possible causes of this, focusing on the relative magnitudes of the effects of elevated [CO2]a on specific leaf (nm) and plant (np) nitrogen concentrations, leaf mass per unit area (h), and plant nitrogen productivity (α). In our survey of 39 studies with 35 species, we found that elevated [CO2]a led to decreased nm and np in all the studies and to increased h and α in most of the studies. The magnitudes of these changes varied with species and with experimental conditions. Based on a model that integrated [CO2]a-induced changes in leaf nitrogen into a biochemically based model of leaf photosynthesis, we predicted that, to a first approximation, photosynthesis will be upregulated (Amax will increase) when growth at increased [CO2]a leads to increases in h that are larger than decreases in nm. Photosynthesis will be downregulated (Amax will decrease) when increases in h are smaller than decreases in nm. The model suggests that photosynthetic capacity increases at elevated [CO2]a only when additional leaf mesophyll more than compensates the effects of nitrogen dilution. We considered two kinds of regulatory paradigms that could lead to varying responses of ηR to elevated [CO2]a, and compared the predictions of each with the data. A simple static model based on the functional balance concept predicts that ηR should increase when neither np nor h is very responsive to elevated [CO2]a. The quantitative and qualitative agreement of the predictions with data from the literature, however, is poor. A model that predicts ηR from the relative sensitivities of photosynthesis and relative growth rate to elevated [CO2]a corresponds much more closely to the observations. In general, root fraction increases if the response of photosynthesis to [CO2]a is greater than that of relative growth rate. 相似文献
87.
In this study we investigated Arabidopsis thaliana (L.) Heynh. inflorescence development by characterizing morphological changes at the shoot apex during the transition to flowering. Sixteen-hour photoperiods were used to synchronously induce flowering in vegetative plants grown for 30 d in non-inductive 8-h photoperiods. During the first inductive cycle, the shoot apical meristem ceased producing leaf primordia and began to produce flower primordia. The differentiation of paraclades (axillary flowering shoots), however, did not occur until after the initiation of multiple flower primordia from the shoot apical meristem. Paraclades were produced by the basipetal activation of buds from the axils of leaf primordia which had been initiated prior to photoperiodic induction. Concurrent with the activation of paraclades was the partial suppression of paraclade-associated leaf primordia, which became bract leaves. The suppression of bract-leaf primordia and the abrupt initiation of flower primordia during the first inductive photoperiod is indicative of a single phase change during the transition to flowering in photoperiodically induced Arabidopsis. Morphogenetic changes characteristic of the transition to flowering in plants grown continuously in 16-h photoperiods were qualitatively equivalent to the changes observed in plants which were photoperiodically induced after 30 d. These results suggest that Arabidopsis has only two phases of development, a vegetative phase and a reproductive phase; and that the production of flower primordia, the differentiation of paraclades from the axils of pre-existing leaf primordia and the elongation of internodes all occur during the reproductive phase. 相似文献
88.
Juichi Yamagiwa Ndunda Mwanza Takakazu Yumoto Tamaki Maruhashi 《Primates; journal of primatology》1994,35(1):1-14
Details are presented of the composition of the diet of eastern lowland gorillas, derived mainly from a study of their fresh
trails and fecal analysis, during the course of an entire year in the tropical lowland forests of the Itebero region, Zaire.
Gorillas ate 194 plant foods from 121 species and 45 families. They consumed 48 species of fruits; and 89% of fecal samples
contained fruit seeds, but fruits were a relatively small part (25%) of the total number of food items. The composition of
their diet changed seasonally. When consumption of fruit decreased in the long rainy and the long dry seasons, the gorillas
ate, in addition to Zingiberaceae and Marantaceae, many kinds of leaf and bark, which may be an important buffer against the
shortage of fruits. Gorillas also fed regularly on ants (Ponerinae), and the frequency of consumption showed small seasonal
variations. From a comparison of diet composition, eastern lowland gorillas appeared to be intermediate between the other
two subspecies. The choice of food showed differences in preference of fruits and insects between subspecies and may reflect
high similarities within subspecies in lowland and montane forests. 相似文献
89.
Seasonal changes in the spermatogenic epithelium of adult Japanese macaques (Macaca fuscata fuscata)
Tomoo Enomoto Kiyoaki Matsubayashi Yasukazu Nagato Mayumi Nakano 《Primates; journal of primatology》1994,35(4):465-472
A histological study was undertaken to clarify seasonal changes in the spermatogenic epithelium of Japanese macaques. Testicular
tissue samples were excised by biopsies from five adult laboratory-maintained males in mating and non-mating seasons. The
samples were fixed with Bouin's solution, embedded in paraffin, and stained with PAS and hematoxylin. Microscopic observations
on cross-sections of seminiferous tubules revealed that the seminiferous epithelium in the mating season was thicker than
in the non-mating season. PAS-stained granules were found in some of the dark A-type spermatogonia, which significantly increased
in the non-mating season. Spermatids of the steps preceding the appearance of the acrosomic cap in stages I to III were observed
significantly more often than those in the step coinciding with the formation of the acrosomic cap in stage IV. In stage I,
the ratio of mature spermatids or spermatozoa to immature spermatids in the mating season was higher than that in the non-mating
season. These findings suggest that spermiogenesis, as well as spermatocytogenesis, is inhibited in the non-mating season. 相似文献
90.