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141.
142.
研究了应用拮抗酵母菌丝孢酵母(Trichosporon pullulans(Lindner.)Diddens et Lodder)、罗伦隐球酵母(Cryptococcus laurentii(Kuffer.)Skinner)和粘红酵母(Rhodotorula glutinis(Fresenius)Harrison)后拮抗菌在果实表面的繁殖能力以及对不同贮藏条件下甜樱桃(Pranus avivum L.cv.Hongdeng)果实采后病害的防治效果。酵母菌的使用浓度为1×10~8CFU/mL。结果表明,田间3种拮抗菌都能够在果实表面增值,但是只有C.laurentii和R.glutinis能够持续稳定地生长。C.laurentii的抑病效果最好,它对田间环境和采后低温低氧及高CO_2都具有很强的适应能力。 相似文献
143.
Ethanol at 50% (v/v) and sodium bicarbonate at 1% (wt/v), either alone or in combination, were applied to organically grown strawberries 1 h before harvest to control the natural incidence of postharvest diseases. Botrytis cinerea was the major cause of decay in all of the experiments. In three experiments, ethanol significantly reduced the decay incidence after storage for 3 days at 1°C followed by 2 days at 24°C, while the efficacy of sodium bicarbonate was inconsistent. The combination of ethanol and sodium bicarbonate did not increase their efficacy. Postharvest hot water dips at 55 and 60°C for 30 s significantly reduced the decay incidence to 3.4 and 2.7%, respectively, while decay incidence in the control was 28.5% (the first experiment). The efficacy of the hot water treatments at 55 and 60°C for 30 s was consistent in three experiments. In the third experiment, the efficacy of hot water treatment at 60°C was significantly higher than that of hot water treatment at 55°C. All pre‐ and postharvest treatments significantly reduced natural fungal populations on the surfaces of fruits. None of the pre‐ and postharvest treatments caused surface injuries to the fruit or adversely affected weight loss and taste parameters. 相似文献
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145.
Nonsense-mediated mRNA decay (NMD) is the best-characterized mRNA surveillance mechanism; this process removes faulty mRNAs harboring premature termination codons (PTCs). NMD targets newly synthesized mRNAs bound by nuclear cap-binding proteins 80/20 (CBP80/20) and exon junction complex (EJC), the former of which is thought to recruit the ribosome to initiate the pioneer round of translation. After completion of the pioneer round of translation, CBP80/20 is replaced by the cytoplasmic cap-binding protein eIF4E, which mediates steady-state translation in the cytoplasm. Here, we show that overexpression of eIF4E-T preferentially inhibits cap-dependent steady-state translation, but not the pioneer round of translation. We also demonstrate that overexpression of eIF4E-T or Dcp1a triggers the movement of eIF4E into the processing bodies. These results suggest that the pioneer round of translation differs from steady-state translation in terms of ribosome recruitment. 相似文献
146.
Yamochi T Ohnuma K Hosono O Tanaka H Kanai Y Morimoto C 《Biochemical and biophysical research communications》2008,370(1):195-199
We identified human decapping enzyme 2 (hDCP2) as a binding protein with Ro52, being colocalized in processing bodies (p-bodies). We also showed that the N-terminus and C-terminus of Ro52 bound to hDCP2. Moreover, Ro52 enhanced decapping activity of hDCP2 in a dose-dependent manner. Our data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation. 相似文献
147.
Giovannelli A Di Marco S Maccarone R Bisti S 《Biochemical and biophysical research communications》2008,365(2):349-354
Recent data challenged the assumption that light has little effect on retina development. Here, we report evidence that dark rearing permanently changes the synaptic input to GCs. A reduced spontaneous postsynaptic currents (SPSCs) frequency was found in retinal GCs from rats born and raised in the dark for three months. Glutamate antagonists (CNQX and AP-5) reversibly reduced SPSCs frequency in control and dark-reared (DR) retinae. The GABA antagonist picrotoxin (PTX) reduced SPSCs frequency in control retinas, but increased SPSCs frequency in DR, mainly by presynaptic action on excitatory currents. In DR animals exposed to normal cyclic light for 3 months, SPSCs frequency remained lower then in control rats and increased following PTX, suggesting that long-term dark rearing induces permanent modifications of the retinal circuitry. Our results strongly support the idea that light stimulation plays a role in establishing normal synaptic input to GCs. 相似文献
148.
149.
Harlan W. Stockman 《Soil & Sediment Contamination》1998,7(5):615-647
The solubility of plutonium was estimated for waste buried at the Greater Confinement Disposal site in Nevada. The EQ3/6 thermochemical database was modified to include recent data on Pu complex formation, and the solubilities of two critical phases (probertite (CaNaB5O9·5H2O), added as a backfill material; and Ca sac-charate) were determined by experiment. Reaction path runs were used to model effects of cellulose degradation, including complexation of actinides by organic acids and carbonate, decay of the complexing agents, and the buildup and diffusive loss of CO2 through the permeable alluvium. For most waste interaction scenarios, long-term (≈103 years) concentrations of Pu in pore waters are ≤10?7 molal and are dominated by carbonate complexes, although organic complexes could dominate in the first ≈103 years. In unusual circumstances, carbonation of buried lithium could produce very high Pu solubilities; however, even in such a system, a slight lowering of the effective redox potential dramatically lowers Pu solubility. A sensitivity analysis suggests that the “base case” calculations are conservative, tending to overestimate long-term solubility, with the system redox and the identity of the organic acids the major sources of uncertainty. 相似文献
150.