Clarification of animal cell cultures on a large scale by continuous centrifugation

Summary Animal cells from 80-L and 2000-L fed batch fermentations were removed by a prototype disc stack centrifuge in order to achieve a fast and reliable separation of solids from large quantities of cell culture fluids. The clarification capacity was excellent for animal cells but particles remained in the liquid phase and affected further downstream processing of the cell-free harvest fluid. No significant loss of product was observed. A number of parameters were monitored to optimize process conditions for use with animal cells.     

畜禽遗传资源系统保存的理论与模拟实验研究──I．畜禽遗传资源系统保存的理论分析

本文提出了畜禽遗传资源系统保存的概念、基本思想及其群体遗传结构变化的数学模型。该理论是将一定时空内某一畜种所拥有的全部基因作为保存对象,既将活体保存作为基本方法,又将其有机地与高新生物技术结合在一起;既追求系统地保存控制畜种特性的基因资源,又可达到保存地方品种的目的。在假定无世代重叠,群体内存在选择、突变、迁移,且考虑漂变效应的情况下,本文所构建的两个数学模型可分别用来描述一个座位上多个主基因频率或数量性状群体均数的动态变化。     

我国某些苏铁类生殖器官化石的发现兼论苏铁类起源

本文对苏铁类化石的系统分类作了概要的介绍。重点报道了笔者在近廿年来所采集的苏铁类生殖器官化石,特别是中晚石炭世的一枚苏铁大孢子叶化石的发现,将苏铁类起源的时间大大地推前了,并指出其起源地应在中国西北的甘肃地区。所有这些生殖器官的发现将推动苏铁化石生殖生物学的发展,对其起源、分布、演化和苏铁植物区系的形成和发展提供了极为重要的材料。     

犬囊状动脉瘤模型的制作

本文报道用“静脉囊镶嵌技术·制成犬的囊状动脉瘤模型。18个模型(6个单侧型,6个分叉型,6个末梢型)造型后2周经IA DSA检查。本模型在分型、血流动力学改变方面与人类囊状脑动脉瘤类似。不同类型的动脉瘤模型既有相同的血流动力学特征,又有各自的特点,这与动脉瘤与载瘤动脉的角度有关。我们认为该模型可应用于研究动脉瘤的血流动力学与血管内栓塞治疗。     

Induction of apoptosis in oxygen-deprived cultures of hybridoma cells

It is now well documented that apoptosis represents the prevalent mode of cell death in hybridoma cultures. Apoptotic or programmed cell death occurs spontaneously in late exponential phase of batch cultures. Until lately, no specific triggering factors had been identified. Recently, we observed that glutamine, cystine or glucose deprivation induced apoptosis in both hybridoma and myeloma cell lines whereas accumulation of toxic metabolites induced necrotic cell death in these cells. Other triggering factors such as oxygen deprivation might also be responsible for induction of apoptosis. In the present study, induction of cell death by exposure to anoxia was examined in batch culture of the SP2/0-derived hybridoma D5 clone. The mode of cell death was studied by morphological examination of acridine orange-ethidium bromide stained cells in a 1.5 L bioreactor culture grown under anoxic conditions for 75 hours. Under such conditions, viable cell density levelled off rapidly and remained constant for 25 hours. After 45 hours of anoxia, cell viability had decreased to 30% and the dead cell population was found to be 90% apoptotic. In terms of cellular metabolism, anoxia resulted in an increase in the utilization rates of glucose and arginine, and in a decrease in the utilization rate of glutamine. The lactate production rate and the yield of lactate on glucose increased significantly while the MAb production rate decreased. These results demonstrate that glycolysis becomes the main source of energy under anoxic conditions.Cells incubated for 10 hours or less under anoxic conditions were able to recuperate almost immediately and displayed normal growth rates when reincubated in oxic conditions whereas cells incubated for 22 hours or more displayed reduced growth rates. Nonetheless, even after 22 h or 29 h of anoxia, cells reincubated in oxic conditions showed no further progression into apoptosis. Therefore, upon removal of the triggering signal, induction of apoptosis ceased.Abbreviations VNA Viable non-apoptotic cells - VA Viable apoptotic cells - NVNA Nonviable non-apoptotic or necrotic cells - NVA Nonviable apoptotic cells - CF Chromatin-free cells (late nonviable apoptotic cells) - AO Acridine orange - EB Ethidium Bromide - MAb Monoclocnal antibody - D.O. Dissolved oxygen - qMAb Specific MAb production rate (mg. (10⁹ cells)^–1.day^–1) - Specific growth rate (h^–1) - X_v Viable cell number (10⁵ cells.mL^–1) - X_t Total cell number (10⁵ cells.mL^–1) - Y_lac/glc Yield coefficient of lactate on glucose (mM lactate produced/mM glucose consumed)     

Automated monitoring of cell concentration and viability using an image analysis system

In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed anin situ microscopic image analysis system with an effective cell recognition algorithm. With a small amount of sample, this system can measure the cell density rapidly and aseptically. In addition, it can measure a cell size histogram including cell debris small particle distribution. These small particles have been found to be related to the viability of the mouse-mouse hybridoma STK1 cell line. By using cell debris small particle density as an indicator of cell viability, the developed system provides non-destructive viability monitoring without trypan blue staining.     

川西峨眉晚白垩世夹关期河流沉积中的痕迹化石群落*

晚白垩世夹关组中的痕迹化石群落至少由12个痕迹属17个痕迹种组成,其中包括5个新痕迹种,即Cystichrtium cuwatitivum,Steinichnus laryus,Paradidymaulichnus emeiertsis,Monmorphichnus lineates和Rusophycus univalvis.这一化石群落主要是无脊椎动物的进食迹、觅食迹、爬迹、停息迹和居住迹,其中多数呈下浮痕和全浮痕保存,并形成于经常干旱的河流环境(大多出现在泛滥平原沉积中).该群落可识别出两个痕迹化石组合,即1) Scoyenia-Steinichnus-Rusophycus组合,它主要产自泛滥平原和漫滩环境;2)Skolithos-Arenicolites组合,它代表一种水道砂坝或曲流砂坝环境.     

Where to invest in road mitigation? A comparison of multiscale wildlife data to inform roadway prioritization

Roads and associated traffic have significant impacts on wildlife, from direct mortality caused by vehicle collisions to indirect effects when wildlife avoid roads, restricting access to important resources. Road mitigation measures such as constructing wildlife passages over or under the road with directional fencing have proven effective at reducing wildlife vehicle collisions while also enabling wildlife to safely cross the road. Highway mitigation projects are led by transportation agencies with a primary purpose of improving motorist safety. More recently, through the discipline of road ecology, considerations have included safe wildlife passage through transportation corridors. To prioritize road sections for mitigation, data sources include animal vehicle collision data collected by transportation agencies and connectivity models generated by wildlife professionals. We used a third data source, pronghorn observations collected by citizen scientists, and demonstrated its value to prioritize potential wildlife mitigation sites. Our results clearly demonstrate a misalignment of road mitigation sites using animal-vehicle collision data and those of rarer species of interest.     

DISCOVERY OF VERTEBRATE FOSSILS AND PALEOLITHIC ARTIFACTS IN DANJIANG SUBMERGING AREA AND ITS IMPLICATIONS

IntheareatobefloodedinthesecondengineeringstagefortheDanjiangreservoir,wediscovered16vertebratefossillocalitiesand52Paleolithicsitesin1994,andcollected603artifactsandmanyfossils.Ofthel6new1yfoundvertebratefossillocalities,threearereptilesitesrepresentedbydinosaureggsandlimbbones,andl3producemamma1s,including4Pale0gene,2Ne0geneand7Quaternarysites'ThemammalianlocalitiesareofPale0cene,Eocene,EarlyMiocene,andPli0ceneorEarlyPleistocene,MiddleandIntePleistocene,respectively.Theyfillinsomestrat…