This work was to investigate the effect of flavonoids from Angelica gigas Nakai on the proliferation and differentiation of PC12 cells. Several solvents including hexane, chloroform, ethyl acetate,
butanol and water consecutively partitioned. We determined the ethanol crude extract of Angelica gigas Nakai. The hexane fraction was shown to contain the highest number of flavonoids as follows; 21.48 mg/g and the composition
of the flavonoids was as follows: 12.24 mg/g of quercetin, 4.39 mg/g of myricetin and 2.58 mg/g of catechin. In addition,
this hexane fraction greatly increased both cell growth and outgrowth of the neurite, and whose effects were three times higher
than those of the other fractions. The length of the neurites was measured as ca. 110 μm in adding 50 μg/mL of the hexane
fraction, which was about the same as the case of adding 50 ng/mL of NGF as a positive control. This result indicates that
the differentiation of PC12 cells by the addition of the hexane fraction was comparable to the case of adding NGF. The hexane
fraction was also determined to prevent apoptosis of PC12 cells by suppressing DNA fragmentation. It is interesting that the
mixture of three major flavonoids, quercetin, myricetin and catechin showed stronger activity on, both PC12 cell growth and
neuritis outgrowth, than when adding each flavonoid alone. We believe this was due to the synergistic effects of the three
flavonoids. The activities of these flavonoids from Angelica gigas Nakai are reported for the first time in this study. 相似文献
Ligusticum chuanxiong and Angelica sinensis have been widely used in traditional Chinese medicine to treat some pathological settings such as atherosclerosis and hypertension. We determined the protective effect of the extract of Ligusticum chuanxiong and Angelica sinensis (ELCAS) on human umbilical vein endothelial cells (ECV304) damage induced by hydrogen peroxide. ECV304 cells were pre-treated with ELCAS and exposed to 5 mM hydrogen peroxide. The results show that ELCAS dose- and time-dependently protected ECV304 cells against hydrogen peroxide damage and suppressed the production of reactive oxygen species (ROS). The decrement of ROS may be associated with increased activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Western blot analysis revealed that ELCAS significantly increased the phosphorylation of ERK and promoted eNOS expression. These observations indicate that ELCAS protected ECV304 cells against hydrogen peroxide damage by enhancing the antioxidative ability, activating ERK and eNOS signaling pathway. Our data also provide new evidence of Ligusticum chuanxiong and Angelica sinensis in preventing both cardiovascular and cerebrovascular diseases. 相似文献
Eleven furocoumarins from Angelica silvestris and Heracleum sphondylium were tested for their antifeedant activity gainst two insect larvae: Mythimna unipuncta and the Colorado beetle Leptinotarsa decemlineata. Angular furocoumarins show a smaller effect that the corresponding linear homologues. The following order of activity is observed for substituents: 4-Methoxybenzofuran, a synthetic analogue shows also medium activity. But the most active component is a sesquiterpene: the known bisabolangelone. 相似文献
White and soft calli were induced from the stemnodes of Angelica dahurica on MS medium containing lmg/L 2,4-D, and subcultured on the same medium with decreased concentration of the hormone for about half a year, until quite a number of embryogenic cell clusters were produced in calli. Protoplasts prepared only from this kind of callus were regenerable. The protoplasts-derived colonies were able to develop into embryos directly or to grow continously into calli as affected by the hormone and, in particular, by osmotic pressure in the culture medium. The embryos either formed directly or via callus stage were all capable of regenerating complete plants under proper culture conditions. 相似文献
Angelica dahurica is an important Chinese herbal medicine plant, and its rhizome is of high medicinal value. In recent years, a severe decline in yield has been observed in Bozhou City (China's largest A. dahurica producing area), Anhui province, China. It showed symptoms of decline, stunting, yellowing and many galls in the roots, which was the characterization of infestation by root‐knot nematodes. A survey of root‐knot nematodes on its roots was conducted in this area from June to September, 2011. Based on our results, the nematode species on A. dahurica was identified as Meloidogyne arenaria by the morphological, biochemical and molecular methods. To our knowledge, this is the first report of M. arenaria on A. dahurica in China. 相似文献