全文获取类型
收费全文 | 187篇 |
免费 | 7篇 |
国内免费 | 2篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 9篇 |
2012年 | 6篇 |
2011年 | 10篇 |
2010年 | 7篇 |
2009年 | 12篇 |
2008年 | 12篇 |
2007年 | 11篇 |
2006年 | 11篇 |
2005年 | 10篇 |
2004年 | 14篇 |
2003年 | 8篇 |
2002年 | 1篇 |
2001年 | 1篇 |
2000年 | 10篇 |
1999年 | 8篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1979年 | 2篇 |
排序方式: 共有196条查询结果,搜索用时 46 毫秒
191.
The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars 总被引:1,自引:0,他引:1
C. D. Li B. G. Rossnagel G. J. Scoles 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(8):1259-1268
Microsatellites have many desirable marker properties. There has been no report of the development and utilization of microsatellite
markers in oat. The objectives of the present study were to construct oat microsatellite-enriched libraries, to isolate microsatellite
sequences and evaluate their level of polymorphism in Avena species and oat cultivars. One hundred clones were isolated and sequenced from three oat microsatellite-libraries enriched
for either (AC/TG)
n
, (AG/TC)
n
or (AAG/TTC)
n
repeats. Seventy eight clones contained microsatellites. A database search showed that 42% of the microsatellite flanking
sequences shared significant homology with various repetitive elements. Alu and retrotransposon sequences were the two largest
groups associated with the microsatellites. Forty four primer sets were used to amplify the DNA from 12 Avena species and 20 Avena sativa cultivars. Sixty two percent of the primers revealed polymorphism among the Avena species, but only 36% among the cultivars. In the cultivars, the microsatellites associated with repetitive elements were
less polymorphic than those not associated with repetitive elements. Only 25% of the microsatellites associated with repetitive
elements were polymorphic, while 46% of the microsatellites not associated with repetitive elements showed polymorphism in
the cultivars. An average of four alleles with a polymorphism information content (PIC) of 0.57 per primer set was detected
among the Avena species, and 3.8 alleles with a PIC of 0.55 among the cultivars. In addition, 54 barley microsatellite primers were tested
in Avena species and 26% of the primers amplified microsatellites from oat. Using microsatellite polymorphisms, dendrograms were constructed
showing phylogenetic relationships among Avena species and genetic relationships among oat cultivars.
Received: 1 November 1999 / Accepted: 14 April 2000 相似文献
192.
Although AHCYL2 (long-IRBIT) is highly homologous to IRBIT, which regulates ion-transporting proteins including the electrogenic Na+-HCO3− cotransporter NBCe1-B, its functions are poorly understood. Here, we found that AHCYL2 interacts with NBCe1-B in bovine parotid acinar cells using yeast two-hybrid, immunofluorescence confocal microscopy and co-immunoprecipitation analyses. Whole-cell patch-clamp experiments revealed that co-expression of AHCYL2 reduces the apparent affinity for intracellular Mg2+ in inhibition of NBCe1-B currents specifically in a HCO3−-deficient cellular condition. Our data unveil AHCYL2 as a potential regulator of NBCe1-B in mammalian cells. We propose that cytosolic ionic condition appropriate for AHCYL2 to function might be different from IRBIT. 相似文献
193.
Joint analysis of recurrent and nonrecurrent terminal events has attracted substantial attention in literature. However, there lacks formal methodology for such analysis when the event time data are on discrete scales, even though some modeling and inference strategies have been developed for discrete-time survival analysis. We propose a discrete-time joint modeling approach for the analysis of recurrent and terminal events where the two types of events may be correlated with each other. The proposed joint modeling assumes a shared frailty to account for the dependence among recurrent events and between the recurrent and the terminal terminal events. Also, the joint modeling allows for time-dependent covariates and rich families of transformation models for the recurrent and terminal events. A major advantage of our approach is that it does not assume a distribution for the frailty, nor does it assume a Poisson process for the analysis of the recurrent event. The utility of the proposed analysis is illustrated by simulation studies and two real applications, where the application to the biochemists' rank promotion data jointly analyzes the biochemists' citation numbers and times to rank promotion, and the application to the scleroderma lung study data jointly analyzes the adverse events and off-drug time among patients with the symptomatic scleroderma-related interstitial lung disease. 相似文献
194.
195.
Sônia Cristina Oliveira Melo Fernanda Amato Gaiotto Fernanda Barbosa Cupertino Ronan Xavier Corrêa Alessandra Maria Moreira Reis Dário Grattapaglia Rosana Pereira Vianello Brondani 《Conservation Genetics》2007,8(6):1269-1271
Caesalpinia echinata, commonly known as Pau-brasil (Brazilwood), the famous tree that named Brazil is native to the Atlantic forest. Men extensively
exploited it ever since discovery and colonial times due to its value as a source of red dye. As a consequence, Brazilwood
is a threatened species with populations reduced to small forest fragments. Ten polymorphic microsatellite loci were developed
from an enriched genomic library. Using fluorescently-labeled primers, a total of 83 alleles were found after analyzing a
sample of 44 trees. These high genetic information content markers should allow detailed investigations of mating systems,
gene flow, population structure and paternity in natural populations. 相似文献
196.
C. MAUDET G. LUIKART D. DUBRAY A. VON HARDENBERG P. TABERLET 《Molecular ecology resources》2004,4(4):772-775
We show that Alpine ibex (Capra ibex) and Corsican mouflon (Ovis musimon) faeces yield useful DNA for microsatellite analysis, however, we detected higher genotyping error rates for spring faeces than for winter faeces. We quantified the genotyping error rate by repeatedly genotyping four microsatellites. Respectively, 99 and 95% of mouflon and ibex genotyping repetitions provided a correct genotype using winter samples, whereas spring samples provided only 52 and 59% correct genotypes. Thus, before starting a noninvasive study, we recommend that researchers conduct a pilot study to quantify genotyping error rates for each season, population and species to be studied. 相似文献