九龙乌头的名实问题

发现九龙乌头A．jiulongense W．T. Wang 即为在我国广泛栽培的药用植物乌头A．carmichaeli Debx.,故予归并。     

国产12种乌头属和18种翠雀属植物的细胞学研究

研究了12种乌头属Aconitum L．和18种翠雀属Delphinium L．植物的染色体。在12种乌头属植物中,除粗花乌头A．crassiflorum为四倍体(2n=4x=32)外,其他种类都为二倍体(2n=2x＝16),中甸乌头 A.piepunense中有B染色体存在,牛扁亚属Aconitum subgen．Lycoctonum的二倍体植物与乌头亚属Aconitum subgen．Aconitum 植物的染色体在大小和形态上有明显区别;所有18种翠雀属植物都为二倍体(2n＝2x=16),其染色体在大小和形态上极为相似,但与乌头亚属的染色体易于区别。翠雀属植物的核型不对称性程度明显高于乌头属植物,因此从染色体证据来看,翠雀属要比乌头属进化。     

Four New Diterpenoid Alkaloids from The Roots of Aconitum coreanum

Four new hetisine‐type C₂₀‐diterpenoid alkaloids, named as coreanines A–D ( 1 – 4 ), were isolated from the roots of Aconitum coreanum, together with thirteen known alkaloids ( 5 – 17 ). Their structures were elucidated by extensive spectroscopic methods including IR, HR‐ESI‐MS and NMR techniques. All the isolated compounds were screened for the acetylcholinesterase (AChE) inhibitory effects, and none of them showed considerable inhibitory activity.     

Production of hairy roots in Aconitum heterophyllum wall. using Agrobacterium rhizogenes

Summary A method for the production of hairy roots of Aconitum heterophyllum wall. is reported for the first time. Embryogenic callus cultures were successfully transformed using Agrobacterium rhizogenes strains viz. LBA 9402, LBA 9360, and A4 for the induction of hairy roots. The transgenic nature of hairy roots was confirmed by mannopine assay using paper electrophoresis. Best growth of transformed roots was obtained on 1/4 MS (Murashige and Skoog, 1962) medium with 3% sucrose. Total alkaloid (aconites) content of transformed roots was 2.96%, which was 3.75 times higher compared to 0.79% in the nontransformed (control) roots. Thin layer chromatography (TLC) analysis of the components of aconites in the transformed roots revealed the presence of heteratisine, atisine, and hetidine.     

Developmental Studies on the Seed and Seedling of Aconitum Kusnezoffii Reichb

The seeds of Aconitum kusnezoffii Reichb. take three months to germinate after shedding. This is due to the fact that the embryo of A. kusnezoffii is not fully developed at the time of shedding, remaining in the stage of heart-shaped or early torpedo-shaped embryo. The germination of seed is epigeal, but in the first year the shoot apex remains in the soil and the short subterraneous stem hibernates with the thickened root-hypocotyl. In the following spring, the stem sprouts out and the axillary bud of the first year's radical leaf develops into a monkshood-tuber. The individual monkshood-tuber is monocarpic, each sprouts out one flowering stem and, at the same time, gives rise to one or sometimes more monkshood-tubers from the underground basal buds, thus, A. kusnezoffii may actually be considered as a “replaced biannial plant”.     

Alkaloids of Aconitum vilmoriniaum var. patentipilum

Seven diterpenoid alkaloids were isolated from Aconitum vilmorinianum var. patentipilum W. T. Wang. Their structures were determined as franchetine(Ⅰ), indaconitine(Ⅱ), yunaconitine(Ⅲ), talatisamine(Ⅳ), chasmaconitine(Ⅴ), crassicauline A (Ⅵ), and bikhaconine(Ⅶ). Last one has not been previously found in nature.     

Comparative Anatomical Studies on Chinese Medicinal Plants of the Genus Aconitum

This paper reports the result of comparative anatomical studies on medicinal roots of 53 species and varieties in the genus Aconitum from China. The anatomical structures of the roots can be classified into 6 types and 18 subtypes according to the level of their evolution. The anatomy of Aconitum roots is discussed in relation to phylogeny based on chemotaxonomy, cytology and other related disciplines. It was found that the six types of Aconitum roots are correlated with chemical components, chromosomes, toxicity and the gross morphology. The relatively advanced Types I and II roots always contain highly toxic alkaloids of diterpenoid diester of aconitine type, distributed mainly in Ser. 3, 5-11 of Sect. Aconitum of Subgen. Aconitum, while the relatively primitive Types III, IV and II roots with small diameters contain mainly atisine and aminal alcohol alkaloids, which have lower toxicity, present in Subgen. Gymnaconitum and Ser. 1, 2 of Sect. Aconitum of Subgen. Aconitum. The primitive Types V and VI roots of Subgen. Lycoctonum contain mainly the most low-toxic alkaloids of lycoctonine Type. The result is of significance both in theory or practice for the development of medicinal plant resourcesand the safety of clinical usage of these of plants.     

On Distributional Features of the Genus Aconitum in Sino-Himalayan Flora

The geographical distribution of Aconitum in the Sino-Himalayan subregion is analysed in the present paper on the basis of taxonomy and relationship between the infrageneric taxa. Asaresult, some conclusions may be arrived as follows: 1. The Sino-Himalayan subregion is the frequency centre and the diversity centre of the genus. For analysis, the distribution area of the genus are assigned to three floristic regions, viz. 1) the East-Asian floristic region, consisting of the Sino-Himalayan subregion and SinoJapanese subregion, 2) the Euro-Siberian region and 3) the North-American region (Table 1). In the East-Asian floristic region, the Sino-Himalayan subregion comprises 3 subgenera, about 5 sections, about 13 series and nearly 180 species. However, the Sino-Japanese subregion has only 2 subgenera, 2 sections. 6 series and about 50 species. The Euro-Siberian region has 2 subgenera, 2 sections, about 9 series and nearly 70 species. The North-American region has 2 subgenera (one of the 2 subgenera has only 1 species), 1 section, 1-2 series and about 26 species. Obviously, the Sino-Himalayan subregion is the richest in taxa. 2. The Sino-Himalayan subregion is not only the preservation centre of the primitive groups and species, but also an actively differentiating region. Largely in the Sino-Himalayan subregion occurs primitive or more primitive tava in the genus, such as Sect. Fletcherum, Sect. Alatosperum and Sect. Sinaconitum, Ser. Tangutica and Ser. Brunnea etc.: A fletcherianum, A. novoluridum, A. chrysotricum, A. brevicalcaratum, A. polycarpus, A. nagarum, A. tanguti cum, A. hookeri, A. naviculare, A. violaceum, etc. On the other hand, the Sino-Himalayan subregion also has the most or relatively advanced taxa, represented by the annual monotypic subgenus Gymnaconitum, A. spiripetalum, A. hamatipetalum and A. bulbitiferum ect. About 50 infraspecific taxa occur in the subregion. For example, A. hemsleyanum has 8 varities, A. franchetii has varities and A. nagarum 1 varity and 2 forms, ect. 3. In the region under discussion the genus Aconitum shows remarkable endemism. The endemic taxa include 3 sections (Sect. Fletcherum, Sect. Alatosperum and Sect. Sina conitum), 3 series (Ser. Brevicalcarata, Ser. Crassiflora and Ser. Bullatifolia) and nearly 150 species, among which primitive and advanced ones are both present. 4. The pattern of geographical distribution of the genus Aconitum shows remarkable relationship between latitude and altitude. The majority of species of this genus prefer habi tats with a cool and more or less constantly moist climate. In the Sino-Japanese subregion, with a higher latitude, the genus has an altitude range of 500-1500 m, whereas in the Sino-Hima-layan subregion the range is 2900-5000 m. To sum up, the Sino-Himalayan subregion is the diversity centre, the frequency centre, the differentiation centre, the preservation centre of the primitive taxa and the centre of endemism of the genus Aconitum, and its development in this subregion has probably been accelerated by the lift of the Himalayas and the complicated environmental conditions.     

乌头及其同属近缘种的SSR指纹图谱研究

乌头(Aconitum carmichaeli Debx.)为中国重要的药用植物,种质资源丰富,同属近缘种繁多。该研究采用SSR分子标记技术对采自17个种群的51份乌头样本和13个同属近缘种的65份样本进行扩增和聚丙烯酰胺凝胶电泳检测,分析了乌头及其同属近缘种的遗传多样性、遗传分化和系统发育关系,筛选出稳定性好、多态性高的SSR引物构建乌头及其近缘种的指纹图谱。结果表明:(1)11对乌头微卫星引物均表现出高的多态性,共检测出109个复等位基因,平均每个位点9.91。(2)乌头在物种水平上遗传多样性丰富(A=3.090 9,I=0.889 7,h=0.540 2),种群间的遗传分化显著(Gst=0.277 4);乌头属14种植物表现出了较高的遗传多样性(A=9.909 1,I=1.526 2,h=0.690 5),物种间遗传分化显著(Fst=0.437),基因流微弱(Nm=0.451 8)。(3)聚类分析表明,同一种群的乌头样本首先聚在一起各自形成小支,17个小支聚为一支;13个乌头近缘种材料中,相同物种的样本分别聚为一支;乌头属14个物种聚为5个大支,与形态分类结果一致。(4)利用基因型丰富、多态性高的6对SSR引物(Tchin03、Tchin04Tchin20、Tchin26、Tchin29、Tchin32)可有效区分14种乌头属植物,并以此建立了乌头种质资源和近缘种的DNA指纹图谱。该研究为乌头的种质资源鉴定及混伪品鉴定提供了重要的技术支撑。     

Two New Species of Aconitum (Ranunculaceae) from Xizang

Two species of the genus Aconitum (Ranunculaceae), Anovoaxillare and Abasitruncatum, are described as new from Xizang Autonomous Region, China. The diagnostic differences between them and their allies are given.