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991.
992.
Chichi Li Kewei Chen Minghui Jia Xi Ding Zipei Jiang Liqun Li Dan Zhang 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2018,1863(12):1498-1510
Some studies have shown that transplanted fat tissues usually cannot survive for long if adipose-derived stem cells (ADSCs) are removed from the tissues in advance. It is more meaningful to explore the mechanism mediating survival and differentiation of ADSCs in the transplanted microenvironment. AMP-activated protein kinase (AMPK) has been shown to be one of the energy receptors that regulate many aspects of cellular metabolism. AMPK activation has been implicated in models of adult ischemic injury, but the mechanism and the regulating effects of AMPK on survival and adipogenesis of transplanted ADSCs are still little known. In this study, we simulated the transplanted microenvironment using oxygen-glucose deprivation (OGD) to test the survival and adipogenesis of ADSCs. We found that OGD treatment triggered significant apoptosis and promoted autophagy. Simultaneously, OGD hindered the differentiation of ADSCs into mature adipocytes. After inhibiting AMPK, the OGD-induced apoptosis rate increased but autophagy was inhibited. The adipogenesis level also decreased. To show that the effects of AMPK on apoptosis and adipogenesis were autophagy-dependent, we pre-inhibited or pre-promoted autophagy with siATG7 or rapamycin while blocking AMPK. We found that inhibiting or improving autophagy exacerbated or alleviated the role of AMPK prohibition in apoptosis and adipogenesis. Furthermore, we showed that AMPK inhibition significantly lowered ULK1 activity but promoted mTOR activity, so that to inhibit autophagy. Our study shows that AMPK plays a protective role in maintaining survival and adipogenesis of OGD-challenged ADSCs partly by positively regulating autophagy. AMPK positively regulates autophagy by inhibiting mTOR but promoting ULK1 activity in OGD condition. 相似文献
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Jiandong Fan Yunping Ma Cuiling Zhang Chong Liu Wenzhe Li Ruud E. I. Schropp Yaohua Mai 《Liver Transplantation》2018,8(16)
Thermal degradation in perovskite solar cells is still an unsettled issue that limits its further development. In this study, 2‐(1H‐pyrazol‐1‐yl)pyridine is introduced into lead halide 3D perovskites, which allows 1D–3D hybrid perovskite materials to be obtained. The heterostructural 1D–3D perovskites are proved to be capable of remarkably prolonging the photoluminescence decay lifetime and suppressing charge carrier recombination in comparison to conventional 3D perovskites. The intrinsic properties of thermodynamically stable yet kinetically labile 1D materials allow the system to alleviate the lattice mismatch and passivate the interface traps of heterojunction region of 1D–3D hybrid perovskites that may occur during the crystal growth process. Importantly, the as‐fabricated 1D–3D perovskite solar cells display a thermodynamic self‐healing ability, which is induced through blocking the ion‐migration channels of A‐site ions by the flexible 1D perovskite with less densely close‐packed structure. Particularly, the power conversion efficiency of as‐fabricated unencapsulated 1D–3D perovskite solar cells is demonstrated to be reversible under temperature cycling (25–85 °C) at 55% relative humidity, which largely outperforms the pure 3D perovskite solar cell. The present study provides a facile approach to fabricate 1D–3D perovskite solar cells with high efficiency and long‐term stability. 相似文献
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997.
Maria Ieda Siqueira Linhares Yoshito Eizuru Gildete Patriota de Andrade Ivailda Barbosa Fonseca Luiz Bezerra Carvalho Ivanildo Tenorio Moreira Yoichi Minamishima 《Microbiology and immunology》1994,38(6):475-478
The seroprevalence of human T cell leukemia virus type 1 (HTLV-1) infection was investigated in Brazilians (570): native inhabitants (298) and descendants from Japanese (272) living in Recife and its neighborhoods—North-east of Brazil. Furthermore, polytransfused renal transplanted patients (54) were also examined for the serological status to this virus. The seropositivity to HTLV-1, screened by enzyme-linked immunosorbent assay (ELISA), was low: 1.34% for the local population and 0.73% for the descendants from Japanese. However, the seropositivity for the renal transplanted patients was found to be 11.1%. This higher value suggests that this retrovirus infection seems to be of importance in this clinical condition. 相似文献
998.
为制备用地高辛精(Digoxigenin,Dig)标记的酶氨酸羟化酶(TyrosineHydroxylase,TH)RNA探针,本研究用分子生物学技术重组质粒PGEMTHI,即分别将携带T7和Sp6启动子的PGEM-3zf质粒和携带TH基因的PKSTH质粒用限制性内切酶消化并行分离纯化,得到带T7和sp6启动子的DNA片段和TH基因片段;经T4DNA连接酶连接后转入大肠杆菌,得到pGEMTH1重组克隆。经小量提取质粒井用酶切分析检测,证实其确有TH基因且方向正确。将此质粒再经限制性内切酶消化则得到线状DNA片段,用T7RNA聚合酶转录合成带有Dig标记的高比活度的单链RNA探针;经斑点杂交试验证实该探针具有较高的可靠性。这将为基因治疗帕金森氏病动物模型的疗效检测提供有效手段。 相似文献
999.
Crystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide. 总被引:20,自引:5,他引:20 下载免费PDF全文
E. A. Merritt S. Sarfaty F. van den Akker C. L'Hoir J. A. Martial W. G. Hol 《Protein science : a publication of the Protein Society》1994,3(2):166-175
Cholera toxin (CT) is an AB5 hexameric protein responsible for the symptoms produced by Vibrio cholerae infection. In the first step of cell intoxication, the B-pentamer of the toxin binds specifically to the branched pentasaccharide moiety of ganglioside GM1 on the surface of target human intestinal epithelial cells. We present here the crystal structure of the cholera toxin B-pentamer complexed with the GM1 pentasaccharide. Each receptor binding site on the toxin is found to lie primarily within a single B-subunit, with a single solvent-mediated hydrogen bond from residue Gly 33 of an adjacent subunit. The large majority of interactions between the receptor and the toxin involve the 2 terminal sugars of GM1, galactose and sialic acid, with a smaller contribution from the N-acetyl galactosamine residue. The binding of GM1 to cholera toxin thus resembles a 2-fingered grip: the Gal(beta 1-3)GalNAc moiety representing the "forefinger" and the sialic acid representing the "thumb." The residues forming the binding site are conserved between cholera toxin and the homologous heat-labile enterotoxin from Escherichia coli, with the sole exception of His 13. Some reported differences in the binding affinity of the 2 toxins for gangliosides other than GM1 may be rationalized by sequence differences at this residue. The CTB5:GM1 pentasaccharide complex described here provides a detailed view of a protein:ganglioside specific binding interaction, and as such is of interest not only for understanding cholera pathogenesis and for the design of drugs and development of vaccines but also for modeling other protein:ganglioside interactions such as those involved in GM1-mediated signal transduction. 相似文献
1000.
质体醌重组的D1/D2/Cytb559复合物的荧光衰减动力学和光破坏作用 总被引:3,自引:0,他引:3
光系统Ⅱ反应中心D1/D2/Cytb559 在分离纯化过程中失去了电子受体QA 和QB,人工合成的质体醌可以与D1/D2/Cytb559 复合物发生重组。癸基质体醌(DPQ)与D1/D2/Cytb599 复合物的重组导致该复合物的荧光强度下降及发射光谱蓝移,同时两个与光化学活性相关的长寿命(24 ns和73 ns)荧光衰减组分占整个荧光的百分数下降,这些结果表明DPQ作为Pheo- 的电子受体,限制了P680+ ·Pheo- 的电荷重组。DPQ 的加入对D1/D2/Cytb559复合物中Chla 分子的光破坏敏感性影响不大,但β-胡萝卜素在加入DPQ 之后可以被光照破坏,这个过程可能与β-胡萝卜素的生理功能相关。 相似文献