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41.
42.
Jun-Ichi Nakamura Shin Yazawa Toshikazu Hada Takayuki Asao Hiroshi Naitoh Seiichi Takenoshita Masaaki Kosaka Suguru Akamatsu Tetsuya Tachikawa Yukio Nagamachi 《Glycoconjugate journal》1997,14(1):81-87
Levels of fucosylated antigens in sera from patients with liver diseases were examined by a newly developed sandwich-type enzyme immuno assay with the aid of anti-fucosylated antigen antibody, YB-2 which reacts simultaneously with Y, Leb and H type 2 antigens. When the cut-off value was set arbitrarily at mean [3 SD values of normal, 30 (69.8%) of the 43 patients with HCC, 14 (53.8%) of the 26 patients with liver cirrhosis (LC) and 24 (45.3%) of the 53 patients with chronic hepatitis (CH) were found to be positive, whereas all of the 30 samples from healthy controls were negative. The levels of 55j732l711p0508/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) in HCC were not correlated with those of YB-2 antigens. The positive rates of the combination YB-2 and AFP assay and YB-2 and PIVKA-II assay in HCC were significantly higher (83.7 and 86.0%, respectively) than that of the AFP and PIVKA-II combination (65.1%) which had been reported to be the best combination up to this time. 相似文献
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Tove Olafsen Charlotte K. Munthe Lund Øyvind S. Bruland Inger Sandlie Terje E. Michaelsen 《Cancer immunology, immunotherapy : CII》1999,48(7):411-418
Osteosarcoma is the commonest malignant tumour of the bones. The presence of micrometastases at the time of primary diagnosis
is associated with poor prognosis. Despite developments in surgery and aggressive chemotherapy, about 50% of the patients
still succumb to the disease. Thus, there is a need to develop alternative treatment modalities. One such strategy is to use
antibodies with improved effector functions. The two monoclonal antibodies, TP-1 and TP-3, recognize a tumour-associated antigen
on human osteosarcoma cells. In the present study, we describe the cloning of the TP-1 variable genes, and the production
of complete chimeric mouse/human monoclonal antibodies. Constructs containing the constant genes from human IgG1, IgG3 or
a mutant IgG3 with a shortened hinge region, called m15, were expressed in the mouse myeloma cell line, NS0. The m15 mutant
has been shown to be very potent in triggering complement-mediated lysis. Our goal was to investigate whether this mutant
could overcome the complement protection on human osteosarcoma cells, which is generally present on all human cells. We found
that the target cells expressed several membrane-bound complement inhibitors, and that masking of these inhibitors rendered
the cells sensitive to lysis. The m15 mutant exhibited greater lytic activity than both IgG3 and IgG1, although it could not
cause extensive killing of the target cells alone.
Received: 21 January 1999 / Accepted: 3 June 1999 相似文献
45.
Archaea have recombination proteins similar to those of eukaryote, but many have not been characterized. Here, the characterization
of a Rad55 homologue from Sulfolobus tokodaii (stRad55A) was reported. StRad55A protein preferred binding to ssDNA and had ssDNA-dependent ATPase activity. In addition,
UV light could induce the expression of this protein, which was different from RadB, a RadA paralog found in euryarchaeota.
Most importantly, stRad55A could release the suppression of excessive stSSB (single strand DNA binding protein from S. tokodaii) on the strand exchange catalyzed by stRadA (RadA homologue from S. tokodaii), by interacting directly with both stRadA and stSSB. StRad55A may function as a mediator to accelerate the displacement
of stSSB by stRadA.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
46.
Sung Joon Kim 《Journal of molecular biology》2009,391(2):414-291
Solid-state NMR has been used to examine the binding of N′-4-[(4-fluorophenyl)benzyl)]chloroeremomycin, a fluorinated analogue of oritavancin, to isolated protoplast membranes and whole-cell sucrose-stabilized protoplasts of Staphylococcus aureus, grown in media containing [1-13C]glycine and l-[?-15N]lysine. Rotational-echo double-resonance NMR was used to characterize the binding by estimating internuclear distances from 19F of oritavancin to 13C and 15N labels of the membrane-associated peptidoglycan and to the 31P of the phospholipid bilayer of the membrane. In isolated protoplast membranes, both with and without 1 M sucrose added to the buffer, the nascent peptidoglycan was extended away from the membrane surface and the oritavancin hydrophobic side chain was buried deep in the exposed lipid bilayer. However, there was no N′-4-[(4-fluorophenyl)benzyl)]chloroeremomycin binding to intact sucrose-stabilized protoplasts, even though the drug bound normally to the cell walls of whole cells of S. aureus in the presence of 1 M sucrose. As shown by the proximity of peptidoglycan-bridge 13C labels to phosphate 31P, the nascent peptidoglycan of the intact protoplasts was confined to the membrane surface. 相似文献
47.
ERC‐55, encoded from RCN2, is localized in the ER and belongs to the CREC protein family. ERC‐55 is involved in various diseases and abnormal cell behavior, however, the function is not well defined and it has controversially been reported to interact with a cytosolic protein, the vitamin D receptor. We have used a number of proteomic techniques to further our functional understanding of ERC‐55. By affinity purification, we observed interaction with a large variety of proteins, including those secreted and localized outside of the secretory pathway, in the cytosol and also in various organelles. We confirm the existence of several ERC‐55 splicing variants including ERC‐55‐C localized in the cytosol in association with the cytoskeleton. Localization was verified by immunoelectron microscopy and sub‐cellular fractionation. Interaction of lactoferrin, S100P, calcyclin (S100A6), peroxiredoxin‐6, kininogen and lysozyme with ERC‐55 was further studied in vitro by SPR experiments. Interaction of S100P requires [Ca2+] of ~10?7 M or greater, while calcyclin interaction requires [Ca2+] of >10?5 M. Interaction with peroxiredoxin‐6 is independent of Ca2+. Co‐localization of lactoferrin, S100P and calcyclin with ERC‐55 in the perinuclear area was analyzed by fluorescence confocal microscopy. The functional variety of the interacting proteins indicates a broad spectrum of ERC‐55 activities such as immunity, redox homeostasis, cell cycle regulation and coagulation. 相似文献
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49.
Alvarez-Fernández M Halim VA Aprelia M Laoukili J Mohammed S Medema RH 《The Journal of biological chemistry》2011,286(38):33029-33036
50.