全文获取类型
收费全文 | 111638篇 |
免费 | 20453篇 |
国内免费 | 3461篇 |
专业分类
135552篇 |
出版年
2024年 | 170篇 |
2023年 | 1130篇 |
2022年 | 1864篇 |
2021年 | 2774篇 |
2020年 | 4635篇 |
2019年 | 6356篇 |
2018年 | 6458篇 |
2017年 | 5880篇 |
2016年 | 5645篇 |
2015年 | 5984篇 |
2014年 | 7952篇 |
2013年 | 9390篇 |
2012年 | 6660篇 |
2011年 | 7847篇 |
2010年 | 6321篇 |
2009年 | 5770篇 |
2008年 | 5967篇 |
2007年 | 5558篇 |
2006年 | 5108篇 |
2005年 | 4515篇 |
2004年 | 3896篇 |
2003年 | 3499篇 |
2002年 | 3088篇 |
2001年 | 2186篇 |
2000年 | 1622篇 |
1999年 | 1490篇 |
1998年 | 1186篇 |
1997年 | 1036篇 |
1996年 | 988篇 |
1995年 | 908篇 |
1994年 | 840篇 |
1993年 | 770篇 |
1992年 | 671篇 |
1991年 | 608篇 |
1990年 | 500篇 |
1989年 | 448篇 |
1988年 | 402篇 |
1987年 | 387篇 |
1986年 | 344篇 |
1985年 | 517篇 |
1984年 | 752篇 |
1983年 | 566篇 |
1982年 | 585篇 |
1981年 | 426篇 |
1980年 | 403篇 |
1979年 | 346篇 |
1978年 | 268篇 |
1977年 | 196篇 |
1976年 | 160篇 |
1975年 | 141篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
《Developmental cell》2022,57(18):2221-2236.e5
- Download : Download high-res image (185KB)
- Download : Download full-size image
103.
104.
The distribution of vitamin K epoxidase activity in rough and smooth microsomes has been studied and compared to the prothrombin precursor and vitamin K-dependent carboxylase activity. All three activities were high in rough microsomes as compared to the low levels found in smooth microsomes. The results are in agreement with the suggestion that there might be a linkage between the vitamin K-dependent carboxylation and epoxidation reaction in vivo. 相似文献
105.
Eliane S. Meier Thomas C. Edwards Jr Felix Kienast Matthias Dobbertin Niklaus E. Zimmermann 《Journal of Biogeography》2011,38(2):371-382
Aim During recent and future climate change, shifts in large‐scale species ranges are expected due to the hypothesized major role of climatic factors in regulating species distributions. The stress‐gradient hypothesis suggests that biotic interactions may act as major constraints on species distributions under more favourable growing conditions, while climatic constraints may dominate under unfavourable conditions. We tested this hypothesis for one focal tree species having three major competitors using broad‐scale environmental data. We evaluated the variation of species co‐occurrence patterns in climate space and estimated the influence of these patterns on the distribution of the focal species for current and projected future climates. Location Europe. Methods We used ICP Forest Level 1 data as well as climatic, topographic and edaphic variables. First, correlations between the relative abundance of European beech (Fagus sylvatica) and three major competitor species (Picea abies, Pinus sylvestris and Quercus robur) were analysed in environmental space, and then projected to geographic space. Second, a sensitivity analysis was performed using generalized additive models (GAM) to evaluate where and how much the predicted F. sylvatica distribution varied under current and future climates if potential competitor species were included or excluded. We evaluated if these areas coincide with current species co‐occurrence patterns. Results Correlation analyses supported the stress‐gradient hypothesis: towards favourable growing conditions of F. sylvatica, its abundance was strongly linked to the abundance of its competitors, while this link weakened towards unfavourable growing conditions, with stronger correlations in the south and at low elevations than in the north and at high elevations. The sensitivity analysis showed a potential spatial segregation of species with changing climate and a pronounced shift of zones where co‐occurrence patterns may play a major role. Main conclusions Our results demonstrate the importance of species co‐occurrence patterns for calibrating improved species distribution models for use in projections of climate effects. The correlation approach is able to localize European areas where inclusion of biotic predictors is effective. The climate‐induced spatial segregation of the major tree species could have ecological and economic consequences. 相似文献
106.
Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength 总被引:8,自引:0,他引:8
A simple, rapid, and inexpensive method for the preparation and purification of chloroplast DNA (cpDNA) from pea has been developed. The crucial step is the isolation of chloroplasts in a medium of high ionic strength (I congruent equal to 1.40 M). CpDNA from pea prepared according to this method has successfully been used for restriction enzyme mapping, Southern transfers, and cloning. 相似文献
107.
108.
With the view of incorporating quaternary ammonium salts (QAs) in marine paints, nineteen of these were tested against a community of marine bacteria, at a temperature and salinity close to those of seawater. The concentration of QAs and the length of the main substituting chain are the main parameters affecting the growth and adhesion of bacteria, but the nature of (i) the other chains, (ii) the counter‐ion and (iii) the rings when inserted in the QA molecule also influenced the bacteria. Increasing the concentration of the QAs decreased the growth rate of the bacteria, the maximum cell density at the plateau and the rate of adhesion. The effect of increasing the length of the main chain depended on the range of carbon numbers. Below 7 carbon atoms, the growth rate was not significantly modified, but the numbers of cells at the plateau increased in contrast with the adhesion rate which decreased rapidly. Increasing the length of the chain to between 7 and 16 carbon atoms resulted in a decrease in the growth rate, a decrease and then a stabilisation in the numbers of cells at the plateau and no further change in the adhesion rate. Possibly an increase in growth rate, adhesion rate and in the numbers of cells at the plateau may occur above 16 carbon atoms. In contrast, the length of the other chains influenced positively the cell concentration at the plateau, and more generally the efficiency of QAs decreased substantially when these chains had the same numbers of carbon atoms. QAs with iodide as counter‐ion were more effective than those with chloride or bromide and phenyl was more effective than benzyl as rings inserted in QAs. The minimum inhibitory concentrations (MIC) were often very high if compared to standard methods with laboratory strains, and this can be tentatively explained by the dominance of Gram— bacteria in the community assayed, the development of resistant strains in the cultures used with time and the presence of organic matter in the culture medium. 相似文献
109.
《Bioscience, biotechnology, and biochemistry》2013,77(10):1763-1765
We previously isolated a Serratia marcescens O5: HI Z-54 strain which produces a new reddish-violet pigment, a peptide- ferropyrimine complex. This study showed that polymyxin B enhances the formation of the pigment about threefold. This occurs because polymyxin B in the medium causes the formation of an iron-polymyxin B complex which imposes a low iron stress on the bacteria and, in turn, enhances pigment production. This shows that polymyxin B is both a membrane-disrupting and ionophoric antibiotic. 相似文献
110.
The collective redox activities of transition‐metal (TM) cations and oxygen anions have been shown to increase charge storage capacity in both Li‐rich layered and cation‐disordered rock‐salt cathodes. Repeated cycling involving anionic redox is known to trigger TM migration and phase transformation in layered Li‐ and Mn‐rich (LMR) oxides, however, detailed mechanistic understanding on the recently discovered Li‐rich rock‐salt cathodes is largely missing. The present study systematically investigates the effect of oxygen redox on a Li1.3Nb0.3Mn0.4O2 cathode and demonstrates that performance deterioration is directly correlated to the extent of oxygen redox. It is shown that voltage fade and hysteresis begin only after initiating anionic redox at high voltages, which grows progressively with either deeper oxidation of oxygen at higher potential or extended cycling. In contrast to what is reported on layered LMR oxides, extensive TM reduction is observed but phase transition is not detected in the cycled oxide. A densification/degradation mechanism is proposed accordingly which elucidates how a unique combination of extensive chemical reduction of TM and reduced quality of the Li percolation network in cation‐disordered rock‐salts can lead to performance degradation in these newer cathodes with 3D Li migration pathways. Design strategies to achieve balanced capacity and stability are also discussed. 相似文献