全文获取类型
收费全文 | 5013篇 |
免费 | 211篇 |
国内免费 | 114篇 |
出版年
2023年 | 56篇 |
2022年 | 68篇 |
2021年 | 63篇 |
2020年 | 84篇 |
2019年 | 123篇 |
2018年 | 143篇 |
2017年 | 101篇 |
2016年 | 73篇 |
2015年 | 89篇 |
2014年 | 199篇 |
2013年 | 343篇 |
2012年 | 158篇 |
2011年 | 251篇 |
2010年 | 180篇 |
2009年 | 242篇 |
2008年 | 225篇 |
2007年 | 254篇 |
2006年 | 242篇 |
2005年 | 186篇 |
2004年 | 160篇 |
2003年 | 141篇 |
2002年 | 142篇 |
2001年 | 92篇 |
2000年 | 90篇 |
1999年 | 86篇 |
1998年 | 87篇 |
1997年 | 88篇 |
1996年 | 84篇 |
1995年 | 56篇 |
1994年 | 76篇 |
1993年 | 59篇 |
1992年 | 72篇 |
1991年 | 61篇 |
1990年 | 67篇 |
1989年 | 61篇 |
1988年 | 50篇 |
1987年 | 75篇 |
1986年 | 46篇 |
1985年 | 92篇 |
1984年 | 114篇 |
1983年 | 85篇 |
1982年 | 86篇 |
1981年 | 49篇 |
1980年 | 63篇 |
1979年 | 30篇 |
1978年 | 26篇 |
1977年 | 35篇 |
1975年 | 17篇 |
1974年 | 22篇 |
1973年 | 18篇 |
排序方式: 共有5338条查询结果,搜索用时 19 毫秒
61.
Richard B. Rothman Uwe K. Schumacher Candace B. Pert 《Journal of neurochemistry》1984,43(4):1197-1199
Abstract: (β-FNA, the β -fumaramate methyl ester of naltrexone, has been shown to antagonize irreversibly the actions of morphine on the guinea pig ileum and mouse vas deferens bioassays but does not affect the actions of δ-receptor ligands on the mouse vas deferens bioassay, suggesting that the compound does not irreversibly bind to the S receptor. In this paper we examine the effect of (β -FNA on the binding of the prototypic δ agonists, Leuenkephalin and d -Ala2 - d -Leu5 -enkephalin, its metabolically stable analogue, and show that treatment of membranes with β -FNA does lead to alterations in the in vitro properties of δ receptors. 相似文献
62.
Effect of Phospholipase Digestion and Lysophosphatidylcholine on Dopamine Receptor Binding 总被引:4,自引:4,他引:0
[3H]Spiperone specific binding by microsomal membranes isolated from sheep caudate nucleus is decreased by trypsin and phospholipase A2 (Vipera russeli), but is insensitive to neuraminidase. The inhibitory effect of phospholipase A2 is correlated with phospholipid hydrolysis. After 15 min of phospholipase (5 micrograms/mg protein) treatment, a maximal effect is observed; the maximal lipid hydrolysis is about 56% and produces 82% reduction in [3H]spiperone binding. Equilibrium binding studies in nontreated and treated membranes showed a reduction in Bmax from a value of 388 +/- 9.2 fmol/mg protein before phospholipase treatment to a value of 52 +/- 7.8 fmol/mg protein after treatment, but no change in affinity (KD = 0.24 +/- 0.042 nM) was observed. Albumin washing of treated membranes removes 47% of lysophosphatidylcholine produced by phospholipid hydrolysis without recovering [3H]spiperone binding activity. However, the presence of 2.5% albumin during phospholipase A2 action (1.5 micrograms/mg protein) prevents the inhibitory effect of phospholipase on [3H]spiperone binding to the membranes, although 28% of the total membrane phospholipid is hydrolysed. Lysophosphatidylcholine, a product of phospholipid hydrolysis, mimics the phospholipase A2 effect on receptor activity, but the [3H]spiperone binding inhibition can be reversed by washing with 2.5% defatted serum albumin. Addition of microsomal lipids to microsomal membranes pretreated with phospholipase does not restore [3H]spiperone stereospecific binding. It is concluded that the phospholipase-mediated inhibition of [3H]spiperone binding activity results not only from hydrolysis of membrane phospholipids, but also from an alteration of the lipid environment by the end products of phospholipid hydrolysis. 相似文献
63.
The conversion of aphid prey tissue (Acyrthosiphon pisum Harris) into predator biomass (immature life stages ofPropylaea 14-punctata L. andCoccinella 7-punctata L.) is calculated by plotting weight gain against assimilation (i.e. consumption minus egestion). This concept is added to
the metabolic pool model byGutierrez
et al. (1981) that enables the simulation of growth and development of a predator on a physiological basis. Physiological time is
expressed in daydegrees above lower development thresholds for both species. Visual examination of observed and calculated
values showed that the model satisfactorily describes the growth patterns of the above predators.
相似文献
64.
A general method for the synthesis of azido-ubiquinone derivatives has been developed directly by substituting one hydrogen atom on the benzoquinone ring with an azido group under weakly acidic conditions. The reaction takes several hours and the yield is generally low. The azido-ubiquinone was purified by preparative thin layer chromatography, and identified by NMR, IR and mass spectra. All the synthesized azido-ubiquinone derivatives show partial activity in mediating biological electron transfer in the dark, and show partial or complete inhibition upon photolysis. 相似文献
65.
Two types of iron-sulfur clusters, [3 Fe–3 S] and [4 Fe–4 S], were identified by 1H-NMR in ferredoxins from Thermus thermophilus, Mycobacterium smegmatis and Pseudomonas ovalis. The [4 Fe–4 S] clusters always showed the redox couples which had potentials lower than that of the [3 Fe–3 S] clusters. 相似文献
66.
Inhibition of epidermal metabolism and DNA-binding of benzo[a]pyrene by ellagic acid 总被引:2,自引:0,他引:2
B J Del Tito H Mukhtar D R Bickers 《Biochemical and biophysical research communications》1983,114(1):388-394
Ellagic acid, a common plant phenol, was shown to be a potent inhibitor of epidermal microsomal aryl hydrocarbon hydroxylase (AHH) activity in vitro, and of benzo[a]pyrene (BP)-binding to both calf thymus DNA in vitro and to epidermal DNA in vivo. The in vitro addition of ellagic acid (0.25-2.0 microM) resulted in a dose-dependent inhibition of AHH activity in epidermal microsomes prepared from control or carcinogen-treated animals. The I50 of ellagic acid for epidermal AHH was 1.0 microM making it the most potent inhibitor of epidermal AHH yet identified. In vitro addition of ellagic acid to microsomal suspensions prepared from control or coal tar-treated animals resulted in 90% inhibition of BP-binding to calf thymus DNA. Application of ellagic acid to the skin (0.5-10.0 mumol/10 gm body wt) caused a dose-dependent inhibition of BP-binding to epidermal DNA. Our results suggest that phenolic compounds such as ellagic acid may prove useful in modulating the risk of cutaneous cancer from environmental chemicals. 相似文献
67.
Heven Sze 《生物化学与生物物理学报:生物膜》1983,732(3):586-594
H+-pumping adenosinetriphosphatases (ATPases, EC 3.6.1.3) were demonstrated in sealed microsomal vesicles of tobacco callus. Quinacrine fluorescence quenching was induced specifically by MgATP and stimulated by EGTA and Cl?. Fluorescence quenching reflected a relative measure of pH gradient formation (inside acid), as it could be reversed by gramicidin (an H+/cation conductor) or 10 mM NH4Cl (an uncoupler). H+ pumping was inhibited by tributyltin (an ATPase inhibitor) and sodium vanadate, but it was insensitive to oligomycin or fusicoccin. The vanadate concentration required to inhibit pH gradient formation was similar to that needed to inhibit KCl-stimulated Mg2+-ATPase activity and generation of a membrane potential (measured by ATP-dependent 35SCN? uptake). About 45% of all three activities (ATPase, pH gradient, membrane potential generation) were vanadate-insensitive, supporting the idea that non-mitochondrial membranes of plants have at least two types of electrogenic H+ pump.A vanadate-insensitive, H+-pumping ATPase previously shown by methylamine accumulation was characterized to be anion-sensitive and possibly enriched in vacuolar membranes (Churchill, K.A. and Sze, H. (1983) Plant Physiol. 71, 610–617). Yet, pH gradient formation determined by quinacrine fluorescence quenching was decreased by monovalent cations with a sequence K+, Rb+, Na+ > Cs+,Li+> choline, bisTris-propane. Since K+ stimulated ATPase activity more than Bistris-propane, K+ appeared to collapse formation of the pH gradient by an H+/K+ countertransport. The sensitivity to vanadate and K+ provides evidence that the plasma-membrane ATPase is an electrogenic H+ pump. 相似文献
68.
Abstract: The pool size and synthesis of phosphatidic acid derived from [2-3 H]glycerol were studied in bovine whole retinas and subcellular fractions. Microsomal preparations from retinas incubated with [2-3 H]glycerol displayed the highest percentage labeling of phosphatidic acid at 5 min of incubation; labeling decreased rapidly thereafter. In drug-treated retinas,0.5 m M propranolol increased the endogenous content of phosphatidic acid and stimulated [2-3 H]glycerol labeling in whole retina and microsomal and postmicrosomal supernatant fractions. This effect was observed during short-term incubations and was reversible. In pulse-chase experiments, 60 min of reincubation greatly reduced the labeling effect, although propranolol still enhanced phosphatidic acid labeling. At the same time, endogenous phosphatidic acid accumulated and reincubation without propranolol reversed the effect. During accumulation, the amount of palmitate increased and that of oleate decreased, whereas the relatively high level of docosahexaenoate in phosphatidic acid remained unchanged. It was concluded that this propranolol-induced effect is due to cationic amphiphilic drug activity in the endoplasmic reticulum that results in a partial inhibition of phosphatidic acid degradation and a stimulation of its de novo synthesis. Hence, net synthesis of phosphatidic acid can be assessed in the retina during short-term incubation with propranolol. 相似文献
69.
Cerebral Blood Flow and Oxidative Metabolism in Conscious Fischer-344 Rats of Different Ages 总被引:1,自引:1,他引:0
Hidenori Takei Wendy R. Fredericks Edythe D. London Stanley I. Rapoport 《Journal of neurochemistry》1983,40(3):801-805
Abstract: The cerebral metabolic rates for O2 and for glucose were measured in conscious, fasted male Fischer-344 rats at the ages of 3, 12, and 24 months, and cerebral blood flow was determined with 14 C-iodoantipyrine. The metabolic rates for oxygen and glucose were obtained by multiplying blood flow by the O2 and glucose concentration differences, respectively, between blood in the femoral artery and in the superior sagittal sinus. Mean cerebral blood flow and the metabolic rates for oxygen and glucose did not differ significantly (p > 0.05) between 3 and 12 or between 12 and 24 months. Nor did the arteriovenous differences for O2 and for glucose change significantly with age. Because the superior sagittal sinus drains blood mainly from the cerebral cortex, the results indicate that average cerebral cortical oxidative metabolism, and the coupling ratios between the cerebral metabolic rate for oxygen and cerebral blood flow and between the cerebral metabolic rate for glucose and cerebral blood flow, do not change significantly with age in the Fischer-344 rat. 相似文献
70.
Antonio G. Gonzalez Jaime Bermejo Barrera Francisco J. Tolfdo Marante 《Phytochemistry》1983,22(4):1049-1050
The fruit bodies of the Basidiomycete Scleroderma polyrhizum have been shown to contain the steroids ergosta-4,6,8(14) 22-tetraen-3-one and 5α,8α-epidoxyergosta-6,22-dien-3β-ol and also palmitic and oleic acids. 相似文献