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951.

Ann Christina Bergmann Gunnar Houen Nicole Hartwig Trier 《Journal of peptide science》2023,29(2):e3450

The chaperone protein calsperin is exclusively expressed in the testes and is essential for sperm migration from the uterus into the oviduct. During spermatogenesis, calsperin interacts with ADAM3, a spermatozoon membrane protein required for fertilization. In this study, we characterized a calsperin epitope by using two monoclonal antibodies and resin-bound calsperin peptides, which were tested for reactivity using a modified enzyme-linked immunosorbent assay. An epitope located at the C-terminal end of calsperin corresponding to amino acids ²²⁸WEKHFLDAS²³⁷ was identified. Three hot spot amino acids were essential for antibody binding whereas the remaining amino acids in the identified epitope appeared to be essential for bringing the critical contact residues into an α-helix structure. No notable sequence similarity was determined between the identified calsperin epitope and calreticulin, a chaperone homologue with sequence similarity, indicating that the identified epitope was specific for calsperin. Characterization of the calsperin epitope and of the two antibodies tested may be used in assays for further characterization of calsperin, where knowledge about the binding sites is necessary, for example, in sandwich assays. Moreover, studies like these may be used to study the function of calsperin during spermatogenesis and fertilization in detail and to develop new male contraception methods by targeting calsperin and mediating neutralization of its function. 相似文献

952.

《Cell reports》2023,42(1):111995

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953.

Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis

Sumnima Singh Jessica A. Thompson Bahtiyar Yilmaz Hai Li Sebastian Weis Daniel Sobral Mauro Truglio Frederico Aires da Silva Sandra Aguiar Ana Rita Carlos Sofia Rebelo Silvia Cardoso Erida Gjini Gabriel Nuñez Miguel P. Soares 《Cell host & microbe》2021,29(3):347-361.e12

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954.

Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients

Yang Li Ming-liang Ma Qing Lei Feng Wang Wei Hong Dan-yun Lai Hongyan Hou Zhao-wei Xu Bo Zhang Hong Chen Caizheng Yu Jun-biao Xue Yun-xiao Zheng Xue-ning Wang He-wei Jiang Hai-nan Zhang Huan Qi Shu-juan Guo Sheng-ce Tao 《Cell reports》2021,34(13):108915

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955.

Computational characterization of epitopic region within the outer membrane protein candidate in Flavobacterium columnare for vaccine development

Manojit Bhattacharya Niladri Mondal Prasanta Patra Bibhuti Bhusan Pal Bidhan Chandra Patra 《Journal of biomolecular structure & dynamics》2020,38(2):450-459

Abstract

Gram-negative bacteria is the main causative agents for columnaris disease outbreak to finfishes. The outer membrane proteins (OMPs) candidate of Flavobacterium columnare bacterial cell served a critical component for cellular invasion targeted to the eukaryotic cell and survival inside the macrophages. Therefore, OMPs considered as the supreme element for the development of promising vaccine against F. columnare. Implies advanced in silico approaches, the predicted 3-D model of targeted OMPs were characterized by the Swiss model server and validated through Procheck programs and Protein Structure Analysis (ProSA) web server. The protein sequences having B-cell binding sites were preferred from sequence alignment; afterwards the B cell epitopes prediction was prepared using the BCPred and amino acid pairs (AAP) prediction algorithms modules of BCPreds. Consequently, the selected antigenic amino acids sequences (B-cell epitopic regions) were analyzed for T-cell epitopes determination (MHC I and MHC II alleles binding sequence) performing the ProPred 1 and ProPred server respectively. The epitopes (9 mer: IKKYEPAPV, YGPNYKWKF and YRGLNVGTS) within the OMPs binds to both of the MHC classes (MHC I and MHC II) and covered highest number of MHC alleles are characterized. OMPs of F. columnare being conserved across serotypes and highly immunogenic for their exposed epitopes on the cell surface as a potent candidate focus to vaccine development for combating the disease problems in commercial aquaculture. The portrayed epitopes might be beneficial for practical designing of abundant peptide-based vaccine development against the columnaris through boosting up the advantageous immune responses.

Communicated by Ramaswamy H. Sarma 相似文献

956.

Development of a T cell-based immunodiagnostic system to effectively distinguish SARS-CoV-2 infection and COVID-19 vaccination status

《Cell host & microbe》2022,30(3):388-399.e3