Identification and Characterization of Phosphorus Use Efficiency in a Doubled Haploid Population of Chinese Spring ~ Lovrin No.lO

It is reported that chromosome 1 R of rye (Secale cereale L) convey phosphorus use efficientgene (s), and 1RS/1BL translocation genotype Lovrin No. 10 is P use efficient. So we hypothesized whetherP efficient gene(s) locate on 1RS, and the high P efficiency of Lovrin No.10 is from 1RS? To test thishypothesis, we investigated the P use efficiency (PUE) of a doubled haploid (DH) population with 61 linesderived from anther culture of F1 hybrid between Lovrin No.10 and phosphorus uptake inefficient genotypeChinese Spring to see whether PUE differs between DH line with and without 1RS/1BL translocation.Acidic polyacrylamide-gel electrophoresis (A-PAGE) of gliadin and genomic DNA in situ hybridization (GISH)were employed to discriminate 1RS/1BL translocation DH lines from the normal 1B DH lines. Among the61 DH lines investigated, A-PAGE analysis showed that 34 lines contained the 1RS/1BL translocationchromosome, which was characterized by the presence of a 1RS-specific Sec- 1 marker bands. Furtherverification with GISH proved that 33 in the 34 lines contained a pair of homozygous 1RS/1BL transloca-tion chromosomes, only one line was a 1RS/1BL monosomic line. A field experiment was carried out on Pdeficient soil to investigate grain yield, biomass, numbers of spikes per plant (SPP), P uptake efficiency(PUpE), and P utilization efficiency (PUtE) of the DH lines and their parents under -P (nil P applied) and P(60 kg P/hm2 applied) at maturity. Results showed soil P deficiency decreased the values of the first fourtraits in Lovrin No.10, but were more severe for Chinese Spring. Lovrin No.10 had higher values of all theabove tested traits at both -P and P than Chinese Spring did, but had similar PUtE with Chinese Spring.These five traits segregated, and differed greatly among DH lines under both -P and P conditions.Although the variations among DH lines exceeded the difference between the two parents, the averagevalues of the DH lines were between the two parents. The average of the above five traits, and P deficiencytolerance (PDT) (measured by relative grain yield of -P/ P) were not different between the DH lines withand without 1RS/1BL translocation. This indicated that there was no association between 1RS and PUEand PDT in Lovrin No.10, and 1RS may not have P efficient gene(s). Therefore, in the offspring of LovrinNo.10, it is possible to combine high PUE and PDT with good quality without the negative effect of 1RS onflour quality.     

枸杞原生质体培养及高效成株体系的建立

由枸杞髓部组织诱导出胚性愈伤组织,并由此愈伤组织建立起稳定的细胞悬浮系。从悬浮细胞游离的原生质体在改良KM培养基(1.5 mg/L 6_BA,0.5 mg/L NAA和0.5 mg/L 2,4_D)中进行液体浅层培养,3～4 d后出现第一次分裂,第7 d统计分裂频率为50.3%,15 d左右可形成细胞团,3～4周后形成肉眼可见的愈伤组织,愈伤组织植板率为1.25%。将细胞团转移到液体分化培养基(MS+6_BA 1.5 mg/L+2,4_D 0.2 mg/L) 8～10 d可形成大量胚状体,及时将胚性愈伤组织块转移到固体分化培养基上(MS+6_BA 0.2 mg/L),可形成大量绿芽,分化率54.17%。绿芽在生根培养基（MS+NAA 0.2 mg/L）可形成完整植株,移栽后成活良好。     

分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响

研究了2．5L罐分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响,确定了分批培养时生产谷胱甘肽合成酶系的最佳pH和最佳温度。研究结果表明：在发酵液的pH为7．2和温度为37℃时,谷胱甘肽合成酶系产量和细胞干重达到最大,分别为690．6U／L和3．77g／L。采用变温控制对菌体的生长和谷胱甘肽合成酶系的合成并没有明显的优点。     

豫北黄河故道湿地鸟类自然保护区鸟类区系调查

1997～1999年对豫北黄河故道湿地鸟类自然保护区的鸟类进行了调查,共记录鸟类119种,隶属16目37科。其中留鸟32种,占总数的26.89％;冬候鸟 24种,占总数的20.17％; 夏候鸟30种,占总数的25.21％; 旅鸟33种,占总数的27.73％。国家一级重点保护鸟类2种,二级重点保护鸟类19种,河南省重点保护的鸟类6种。     

英格兰洞穴苔藓植物区系特征及其岩溶沉积研究

根据作者1998－2000年采自英格兰7个岩溶洞穴452份苔藓植物标本研究,英格兰岩溶洞穴苔藓植物区系具有下列特征：（1）区系种类由20科41属65种（含变种亚种）组成;（2）区系地理成分含北温带分布（52．30％）、温带欧洲分布（15．38％）、欧洲－亚洲分布（1．54％）、欧洲－北美分布（12．31％）、旧世界温带分布（4．62％）、英国特有（1．54％）和世界广泛分布（12．31％）等7种成分;（3）生活型含高丛集型（9．22％）、矮丛集型（20％）、交织型（61．54％）、扇型（4．08％）和平铺型（4．08％）5种类型。14种洞穴苔藓植物参与了洞穴洞口钙华沉积,沉积形态包括钟乳石、洞壁钙华、洞底泉华和洞底滴水钙华4种类型。     

安徽齐云山区蕨类植物区系研究

皖南齐云山区地处中亚热带,自然条件较为优越。蕨类植物丰富,初步统计显示,该山区共有蕨类植物29科,54属,91种9包括亚种和变种）,区系综合系数为-0.592。在相比较的7个山地区系中位居第五,通过对区系组成,性质和特征的分析。表明该区系地理成分复杂多样,属,种均可划分为12种类型（变型）,具有较强的亚热带特性;在区系关系上,该区系与黄山,九华山,天目山,庐山区系关系较为密切,而与武夷山,秦岭,长白山区系关系较为疏远。     

浙江金华蝴蝶种类及区系调查初报

1999－2001年对金华市境内的蝴蝶进行了采集调查,共鉴定整理出6科77种,其中凤蝶科13种,粉蝶科7种,眼蝶科12种,蛱蝶科24种,灰蝶科15种,弄蝶科6种。区系分析结果表明,金华市蝶类以东洋、古北两区共有种为主。     

四川唐家河自然保护区鸟类区系和生态类群

唐家河的鸟类已知有204种,分隶于13目40科,占四川省鸟类615种的33％,古北种87种,占43％,东洋种90种,占445,广布种27种,占13％,特产种27种,占全国特产鸟类98种的28％,占四川省特产鸟类59种的46％,国家一级保护鸟类有4种,二级保护有26种,四川省重点保护鸟类2种,我国和日本国候鸟协定保护鸟类22种,1986年12月采获的鹰雕为四川省鸟类新纪录,1998年10月采获的草为川西北新分布,以此证明该鸟不仅只分布重庆地区。