Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction

Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates micro- tubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory cir- cuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-mo- lecular association, which perturbs the MCAK-TI P150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 inter- action regulates microtubule plasticity to affect the mechanical properties of ceUs during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 ceils. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TI P150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during ceU-in-ceU pro- cesses.     

稿件规范化与标准化

阿拉伯数字的使用 凡是可以使用阿拉伯数字且很得体的地方均应使用阿拉伯数字。世纪、年代、年、月、日、时刻必须使用阿拉伯数字，年份必须用全称。对科技期刊来说，凡处在计量单位和计数单位前面的数字，包括9以下的各位数字，除个别特例外，均应使用阿拉伯数字。不是表示科学计量和有统计意义数字的一位数可以用汉字，例如：一本教材，两种商品等。4位以上（含4位）的数字，采用三位分节法，节和节之间空开1／4格的间距。     

护理本专科生临床实习标准化处理流程库教学法构建

临床实习是医学生学习成长中必然经历的重要过程，传统的医学教学模式和教学方法偏重课本知识，不注重实践技能以及创新能力培养，已对医学生的发展形成禁锢。结合临床实践教学工作实际，以标准化处理和流程库的相关理念和操作方法为支撑，提出了标准化处理流程库的教学方法，改革现行护理本专科生临床实践教学及考核的模式和方法，探索建立以提高临床能力为目的的标准化的训练方法和教学评价体系。