优秀射箭、射击、花泳运动员的脑波涨落图特征之比较

目的：研究运动员的脑波涨落图的特征。方法：实验研究法,数理统计法,脑波涨落图技术。结果：花泳、射箭运动员的S谱线有显著的特异性。结论：运动员脑涨落图特征与运动项目的训练特点相关。     

线蕨属（水龙骨科）的系统发育研究：来自叶绿体rbcL和rps4-trnS序列的证据

线蕨属Colysis 植物主要分布于亚洲热带和亚热带地区,少数种类分布至非洲、澳大利亚(昆士兰)及新几内亚地区.自1849年成立以来,线蕨属的分类范畴和系统位置一直有待确定.本文利用叶绿体基因组的rbcL、rps4基因和rps4-trnS基凶间隔区序列,运用最大简约法和贝叶斯方法分析了线蕨属及其近缘类群的系统演化关系.研究结果显示:(1)线蕨属和薄唇蕨属Leptochilus(含似薄唇蕨属Paraleptochilus)组成一个支持率很高的单系分支(C-L Clade),但是薄唇蕨属的成员位于线蕨属的不同支系内,支持线蕨属和薄唇蕨属合并为一个属;(2)瘤蕨属Phymatosorus单独形成一个单系分支;(3)星蕨属Microsorum是一个多系类群,除Microsorium linguiforme、M.varians和M.pustulatum与马来群岛的Lecanopteris聚在一起外,其他的星蕨属成员均位于不同的支系上.本文的系统发育分析结果为线蕨属和薄唇蕨属的分类处理提供了分子系统学的证据.     

野生二粒小麦籽粒含硒量的差异分析

采用氢化物-原子荧光法,对种植于黔中的来自以色列15个野生二粒小麦群体110个基因型籽粒含硒量进行检测分析.结果表明,15个群体含硒量差异极显著(P≤0.001);110个基因型籽粒硒含量和单粒硒含量分别为0.043～0.409 mg kg-1和0.008～0.125 mg seed-1,平均值分别为0.180 mg kg-1和0.046 mg seed-1.籽粒硒含量和单粒硒含量最低的基因型分别是Gamla群体的TZ120和Mt.Hermon群体的TZ8,最高的基因型分别为Bat-Shelomo群体的TZ36和TZ34.在群体水平上硒浓度差异明显,变异系数CV为9%～74%;斯皮尔曼秩相关分析表明,籽粒硒含量分别与该群体起源地海拔、年平均降雨量、平均干旱天数呈显著负相关,与年均温、8月均温、1月均温呈显著正相关.单粒硒含量的相关分析与籽粒硒含量的相似.野生二粒小麦籽粒含硒量的差异是长期适应环境的结果,其遗传多样性将为小麦硒营养机理研究和育种利用提供材料.     

关于图、表的要求

图要具有自明性,应精选、精制,其内容不可与文字、表格重复。图中的量、单位、符号、缩写词和文字等须与正文一致。病理组织照片,应在文题后的括号内注明染色方法和放大倍数,必要时应附有表示目的物尺寸大小的标度。表一般采用三线式,应力求简洁,主谓合理。表须置于文中相应处,图可附另页。     

高效发酵木糖生产乙醇酵母菌株的构建

获得高效发酵木糖生产乙醇的酵母菌株是木质纤维素生物转化生产燃料乙醇的重要前提。在4%乙醇驯化的基础上,选择了乙醇耐性提高的休哈塔假丝酵母（Candida shehatae）CICC1766菌株进一步进行紫外诱变,得到了木糖发酵性能较强的呼吸缺陷型突变体,并与乙醇发酵性能良好的酿酒酵母（Saccharomyces cerevisiae）ATCC4126进行原生质体融合。采用单亲灭活法对休哈塔假丝酵母原生质体进行紫外灭活,在聚乙二醇（PEG）诱导下融合,对得到的融合子进行木糖发酵能力测定,选择到了一株能够更好地利用木糖产乙醇,并且木糖发酵性能比亲本得到明显提高的融合子F6,此融合子发酵50 g/L木糖,最高乙醇浓度达到18.75g/L,乙醇得率为0.375,达到理论转化值0.511的73.4%。与原始出发菌株CICC1766相比,乙醇产量提高了28%。     

超声造影评价股浅静脉瓣膜功能的初步应用

目的:探讨超声造影股浅静脉瓣膜返流时问与瓣膜功能的关系.方法:回顾性分析35例40侧诊断为原发性深静脉功能不全患者的超声造影资料,按照x线顺行静脉造影瓣膜返流分类分为两组,分别记录股浅静脉瓣膜返流时间,并进行两组的差异性分析.结果:统计学分析表明,轻中度组和重度组的返流时间之间有统计学差异(P＜0.05).结论:超声造影检查股静脉瓣膜返流时间能够比较准确地提供下肢深静脉的功能情况.     

An overview of plant centromeres

The centromere is a defining region that mediates chromosome attachment to kinetochore microtubules and proper segregation of the sister chromatids. Intriguingly, satellite DNA and centromeric retrotransposon as major DNA constituents of centromere showed baffling diversification and species-specific. However, the key kinetochore proteins are conserved in both plants and animals, particularly the centromere-specific histone H3-1ike protein （CENH3） in all functional centromeres. Recent studies have highlighted the importance of epigenetic mechanisms in the establishment and maintenance of centromere identity. Here, we review the progress and compendium of research on plant centromere in the light of recent data.     

风雨同舟--丹江口水库旧石器考察纪实

美丽的丹江口水库位于湖北、河南两省的交界处,地处秦岭南坡、伏牛山和武当山之间,长江、黄河两大流域之间的汉江中上游地区,汉水及其支流丹江由西向东、由北向南的交汇处,水域面积126万亩,蓄水总量81亿立方米。这里水质清澈、透明,是国家重点水利项目南水北调中线工程的源头水     

Reduced cardiac output is associated with decreased mitochondrial efficiency in the non-ischemic ventricular wall of the acute myocardial-infarcted dog

Cardiogenic shock is the leading cause of death among patients hospitalized with acute myocardial infarction (MI).Understanding the mechanisms for acute pump failure is therefore important.The aim of this study is to examine in anacute MI dog model whether mitochondrial bio-energetic function within non-ischemic wall regions are associated withpump failure.Anterior MI was produced in dogs via ligation of left anterior descending (LAD) coronary artery,thatresulted in an infract size of about 30% of the left ventricular wall.Measurements of hemodynamic status,mitochondrialfunction,free radical production and mitochondrial uncoupling protein 3 (UCP3) expression were determined over 24h period.Hemodynamic measurements revealed a>50% reduction in cardiac output at 24 h post infarction when com-pared to baseline.Biopsy samples were obtained from the posterior non-ischemic wall during acute infarction.ADP/Oratios for isolated mitochondria from non-ischemic myocardium at 6 h and 24 h were decreased when compared to theADP/O ratios within the same samples with and without palmitic acid (PA).GTP inhibition of (PA)-stimulated state 4respiration in isolated mitochondria from the non-ischemic wall increased by 7% and 33% at 6 h and 24 h post-infarctionrespectively when compared to sham and pre-infarction samples.This would suggest that the mitochondria are uncoupledand this is supported by an associated increase in UCP3 expression observed on western blots from these same biopsysamples.Blood samples from the coronary sinus measured by electron paramagnetic resonance (EPR) methods showedan increase in reactive oxygen species (ROS) over baseline at 6 h and 24 h post-infarction.In conclusion,mitochondrialbio-energetic ADP/O ratios as a result of acute infarction are abnormal within the non-ischemic wall.Mitochondria ap-pear to be energetically uncoupled and this is associated with declining pump function.Free radical production may beassociated with the induction of uncoupling proteins in the mitochondria.     

3种植物乙醇提取物对南方根结线虫卵囊酯酶活力影响及其成分预分析

2000μg/ml合欢叶、黄花菊花和万寿菊叶乙醇提取物溶液处理根结线虫卵囊24h、48h和120h后,采用酶标仪分别对卵囊羧酸酯酶和乙酰胆碱酯酶活力进行了测定。结果表明各处理对根结线虫卵囊羧酸酯酶和乙酰胆碱酯酶的活性均有显著的抑制作用。与对照无菌水相比,合欢叶、黄花菊花和万寿菊叶乙醇提取物溶液处理卵囊120h后,卵囊羧酸酯酶活力抑制率分别为85.94%、86.16%和65.18%,乙酰胆碱酯酶活力抑制率分别为51.16%、46.51%和34.88%。并对3种植物乙醇提取物化学成分进行预分析。