杜仲的繁殖技术与经济价值

杜仲,又名扯丝皮、丝竹棉、乱银丝、玉丝皮、木棉、思仲和野桑。自古以来,杜仲作为强壮药入药。在《神农本草经》中就把杜仲列为上品,有“治腰腿痛、益精气、坚筋骨,人服轻身耐老”的记载。 杜仲喜光不耐阴,对环境适应性较强,能耐-20℃的低温。在酸性、中性、微碱性及钙质土壤中均能生长。而在土层疏松、肥沃湿润、排     

Biological activities of purified harpin(Xoo) and harpin(Xoo) detection in transgenic plants using its polyclonal antibody

Many harpins have been found in plant pathogen bacteria that can elicit disease and insect resistance in plants, and promote plant growth. In this work, we overexpressed and purified Xanthomonas oryzae pv. oryzae harpin, harpinxoo, in Escherichia coli BL21/pGEX-hpa1. Harpinxoo was fused to the Cterminus of glutathione S-transferase （GST） and purified using the Bulk GST purification module and thrombin cleavage capture kit. Purified harpinxoo protein was sensitive to protease K and stable to heat treatment, and could not induce a hypersensitive response after treatment with various plant metabolic inhibitors; these characteristics were similar to harpinEa of Erwinia amylovora. The purified harpinxoo showed a similar ability to induce tobacco mosaic virus resistance in tobacco as harpinEa. Its antibody worked well in detecting the purified harpinxoo, harpinxoo in the total protein of E. coli BL21/pGEX-hpa1 and an hpal transgenic rice.     

繁殖期高原鼠兔的行为时间分配与后代存活率的关系

2002年4-8月份,在中国科学院海北高寒草甸生态系统定位站附近,采用标志流放、直接观察法和解剖法对青藏高原特有的植食性小哺乳动物———高原鼠兔(Ochotonacurzoniae)的行为时间分配、繁殖特征和后代存活率等方面进行了研究。结果表明,雌、雄成体平均地面活动时间占总时间的比例分别为88.67%和89.88%,在不同的繁殖时段,成体的各种行为时间分配存在显著的变化,并影响后代的存活率。幼体从出生到15d的存活率和雌、雄性成体的每次移动距离以及雌性成体的地面活动强度都显著的正相关;从15d到45d的存活率和雄性成体的地面活动强度、观望强度显著的正相关。在相同的观察时期,高原鼠兔雌、雄成体的一些行为时间分配存在显著差异,如雄性成体地面移动距离、频次在繁殖前期显著的高于雌性成体;雄性成体的观望强度在6、7月份显著高于雌性成体。说明雄性成体在领域防卫中较雌性成体承担更多的责任,雌、雄成体在育幼活动中的繁殖投入存在互补性。     

树莓的组织培养及快速繁殖

以树莓的茎尖为外植体进行组织培养,筛选出各培养阶段适宜的培养基分别为:(1)丛生芽诱导:MS 6-BA 1.0 mg/L;(2)继代培养:MS 6-BA 0.5~1.0 mg/L;(3)生根培养:1/2MS NAA 0.2mg/L或1/2MS IBA0.2 mg/L。     

庐山地区观赏蕨类的引种及其繁殖

庐山位于江西省北部,共有蕨类植物39科87属248种。其中具有观赏价值的种类达102种。讨论了庐山地区观赏蕨类植物的引种情况,共有24科40属59种。其中绝大部分种类生长良好,对其中大部分种类进行了繁殖技术的研究,为进一步利用提供了依据。     

珙桐的引种繁殖研究

珙桐是我国特有的珍稀植物,为保护、发展这一树种,对珙桐进行了引种实验和一系列的繁殖研究。     

草果组织培养快速繁殖育苗研究

以草果（Amomum tsao-ko Crevost et Lemaire）茎尖为外植体,MS为基本培养基,探讨不同浓度的培养基因子,对草果不定芽的诱导、增殖分化、生根情况和试管苗移栽成活率的影响.结果表明：①以MS＋6-BA 6 mg/L＋NAA 0.1 mg/L＋TDZ 0.05 mg/L的培养基对不定芽增殖分化效果较好,增殖倍数达到2.34,不定芽叶色绿,苗粗壮,整体感最好.②糖浓度对草果不定芽分化增殖有一定影响,以糖浓度为30～35g/L增殖分化效果较好.③在草果不定芽生根中,以1/2MS＋IBA 0.2 mg/L、1/2MS＋NAA 0.2 mg/L效果较好.出根率较高,须根最多,平均根数最多,移栽成活率为100%.     

在川藏线上走尽量避开灾害季节

“走在川藏线上,最安全的办法是避开灾害季节”,这是张全林反复强调的一句话。他现任成都军区川藏兵站部部长,军衔大校。从20岁当兵至今,33年来,在这条生死线上往返了150多次。听说,他不知多少次替新兵将车一辆辆开过最险地段。但是出乎意料,坐在我们面前的他,不是单一的英雄色彩,纵然坚强,纵然无畏,但谈到与自然打交道时,他极度冷静和理性的话语,让我们无法不敬佩。如果说川藏线打炼出来的人同时兼备勇敢和理性的话,那么在张全林的身上我们看到了这种品格的完美和极致。[编者按]     

Different Patterns of Changes in the Dry Season Diameter at Breast Height of Dominant and Evergreen Tree Species in a Mature Subtropical Forest in South China

Information on changes in diameter at breast height （DBH） is important for net primary production （NPP） estimates, timing of forest inventory, and forest management. In the present study, patterns of DBH change were measured under field conditions during the dry season for three dominant and native tree species in a monsoon evergreen broad-leaved forest in the Dinghushan Biosphere Reserve. For each tree species, different patterns of DBH change were observed. In the case of the fast-growing tree species Castanopsis chinensis Hance, large diurnal fluctuations occur, with a peak DBH in the early morning （around 05：00 h） that decreases to a minimum by about 14：00 h. Both Schima superba Gardn. et Chemp and Cryptocarya chinensis （Hance） Hemsh exhibited less diurnal swelling and shrinkage. Diurnal fluctuations for these species were observed on a few occasions over the period of observation. Graphical comparisons and statistical analysis of changes in DBH with meteorological variables indicate that for different trees, the different changes in DBH observed responded to different meteorological variables. Large stem changes were found to occur for Ca. chinensis trees that were associated with variations in solar radiation. However, both S. superba and Cr. chinensis were found to be less sensitive to solar radiation. Changes in the DBH of these two species were found to be controlled mainly by soil temperature and soil moisture. During the later dry season, with a lower soil temperature and soil moisture, all three tree species stopped growing and only negligible shrinkage, expansion, or fluctuation occurred, suggesting that the optimum time to measure tree growth in the Dinghushan Biosphere Reserve is the later dry season.