其它

<正>采用下述方法,增加了枸桔(Poncirus trif oliala)不定胚状体品系对NaCl耐性的变异。种子培养在含1.0mg/1蔡乙酸(NAA)加2.5mg/1-节基嗦吟(BA)的培养基上。芽和根之间产生胚状体的过渡区,用0.3%甲基磺酸乙醋处理。,0.5,1.0,和2.0小时,     

食品上的应用

<正>采用酵母突变株NO325(被重新鉴定为雷沃特假丝酵母)使异柠檬酸生产得到改善。采用NTG诱变得到的突变株NO OM-102被选为生产酸的最好菌株。种子培养物采用含葡萄糖、琥珀酸按盐以及酵母膏的培养基制备。于30℃,振荡培养2天。将种子培养物接种到含葡萄糖、盐类、硫胺素盐     

红松阔叶林中红松种子的分布及更新

天然阔叶红松林的红松种子经过动物活动的处理,在林内呈四个层次的垂直分布．其中在地被物下是以簇状埋藏着,数量相当多,尤其在红松密林中高大树木的周围,这些簇状分布的种子成为红松天然更新的基础。在次生阔叶林中红松种子埋藏量则很小。而埋藏位置与红松幼苗适生生境的不吻合是红松天然更新的主要障碍。     

快速、无损大豆种子连续取样技术及其DNA制备

建立简便、快速和无损种子连续取样技术流程及基因型鉴定技术体系,可节约种植成本及缩短鉴定周期,提高基因功能研究和育种效率。该研究利用微型电钻和空气泵等简单装置设计了一种连续且无损钻取大豆(Glycine max)种子组织的方法,并优化了利用384深孔微孔板高通量提取DNA及基因型鉴定技术体系。该方法也可用于水稻(Oryz...     

邢台西部太行山区种子植物区系及与其它山区区系的关系

邢台西部太行山区位于河北省西南部,属于太行山脉南段。区内植物种类丰富,地理成分复杂,具明显的温带性质,同时,与热带又有一定的历史渊源。运用R/T值、区系谱等方法对本区及部分山区地理成分进行比较研究,结果表明邢台西部太行山区植物区系与山西太岳山和中条山、北京东灵山及河北雾灵山关系最为密切,与河北小五台山、甘肃六盘山和崆峒山及吉林长白山关系次之,与湖北神农架和湖南八大公山关系较远,与福建龙栖山关系最远。     

穿龙薯蓣种子离体再生体系构建及其组培苗解剖结构特征

以药源植物穿龙薯蓣种子为外植体,构建优化种子消毒时间以及愈伤组织、不定芽和生根诱导等激素浓度、组成和配比体系,并比较组培苗的叶片、缠绕茎和根状茎等营养器官与实生苗在解剖结构上的差异,初步建立以种子为外植体的再生体系.结果表明:(1)外植体采用2％NaClO消毒15 min和18 min为宜;愈伤组织诱导培养基为MS+1...     

ABA-Mediated Inhibition of Germination Is Related to the Inhibition of Genes Encoding Cell-Wall Biosynthetic and Architecture： Modifying Enzymes and Structural Proteins in Medicago truncatula Embryo Axis

Radicle emergence and reserves mobilization are two distinct programmes that are thought to control germination. Both programs are influenced by abscissic acid （ABA） but how this hormone controls seed germination is still poorly known. Phenotypic and microscopic observations of the embryo axis of Medicago truncatula during germination in mitotic inhibition condition triggered by 10 μM oryzalin showed that cell division was not required to allow radicle emergence. A suppressive subtractive hybridization showed that more than 10% of up-regulated genes in the embryo axis encoded proteins related to cell-wall biosynthesis. The expression of α-expansins, pectin-esterase, xylogucan-endotransglycosidase, cellulose synthase, and extensins was monitored in the embryo axis of seeds germinated on water, constant and transitory ABA. These genes were overexpressed before completion of germination in the control and strongly inhibited by ABA. The expression was re-established in the ABA transitory-treatment after the seeds were transferred back on water and proceeded to germination. This proves these genes as contributors to the completion of germination and strengthen the idea that cell-wall loosening and remodeling in relation to cell expansion in the embryo axis is a determinant feature in germination. Our results also showed that ABA controls germination through the control of radicle emergence, namely by inhibiting cell-wall loosening and expansion.     

Preparation and preliminary application of monoclonal antibodies against Trichokirin-S1, a small ribosome-inactivating peptide from the seeds of Trichosanthes kirilowii

Trichokirin-S1,a small ribosome-inactivating peptide recently purified from the seeds ofTrichosanthes kirilowii,has potential clinical applications because of its small molecular mass.Two stablestrains of hybridomas (1F11 and 2A5) that can secrete highly specific monoclonal antibodies (mAbs) againstTrichokirin-S1 have been developed using the hybridoma technique.The isotypes of these two mAbs,1F11and 2A5,were determined to be IgG_(2a) and IgG_1,respectively.The affinity constants,which were measuredby non-competitive ELISA,were found to be 2.3×10~8 M~(-1) and 2.8×10~8 M~(-1),respectively.An immunoaffinitymethod using 2A5-coupled Sepharose 4B was successfully developed to purify Trichokirin-S1.These twoantibodies have also been used to detect Trichokirin-S1 in Western blot.     

鼎湖山针阔混交林锥栗种子距离制约研究

Janzen-Connell 距离制约假说认为:扩散到远离母树地点的种子,更容易逃避种子捕食者和病原体侵染。验证锥栗(Castanopsis chinensis)种子是否支持该假说,可以预测锥栗种群分布格局,解释锥栗种群更新不良的原因。在鼎湖山针阔混交林内,选择离成年母树3、10和15 m处,进行种子放置实验,以及种子消毒和未消毒埋土实验。在无覆盖实验中,10 m处大种子的消失数始终最大,种子离母树距离和种子大小两个因素对种子消失数均没有显著性影响。在排除哺乳动物实验中,种子放置第一周,种子消失数均很少。整个试验期,种子离母树距离和种子大小两个因素对种子消失数也没有显著性影响。在病原体实验中,埋土前两个月,经过消毒的种子袋中种子均保存完好,没有病原体侵染。埋土的种子从第三个月到第六个月,不同距离处的种子,以及消毒和未消毒处理的种子,被侵染数之间均有显著性差异;被侵染的种子数到第六个月趋于稳定。因此,锥栗种子被脊椎动物取食,不呈现距离制约(Distance-dependent)格局;而被病原体侵染的种子命运则支持距离制约假说。     

海洋鸟类对海洋环境的适应

海洋面积占地球表面的 2 / 3 ,在海洋中生活的生物是海洋的一个重要组成部分 ,如浮游生物、鱼类和底栖生物等 ,也包括海洋鸟类。真正的海鸟只在营巢时利用陆地 ,其它大部分时间在海上度过 ,与内陆完全失去联系 ,为大洋性海鸟 ,它们主要在海上取食 ,如信天翁、贼鸥等 ;当然 ,有一些沿岸生活的海鸟也偶而会到内陆江湖中去 ,即在内陆取食、休息 ,沿海岸取食的种类 ,与内地联系密切 ,属于“半海洋性”海鸟 ,如各种鸥类和燕鸥类。海洋面积虽大 ,但因其环境单一 ,多样性低 ,因而真正的海鸟种类并不多 ,全球共有 2 5 0～2 60种 (有人认为有 2 85种 …