Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in nasopharyngeal carcinoma CNE2 cells

Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important factors causing NPC development. However, the mechanism of the TPA effect on NPC remains unclear. In the present study, cells from a poorly differentiated squamous cell carcinoma NPC cell line, CNE2, were stimulated by TPA and proteomics technology was carried out to find protein discrepancies between control and TPA-treated cells. Results revealed that TPA treatment in CNE2 cells could upregulate the expression of ““““triosephosphate isomerase““““ and ““““14-3-3 protein sigma““““ and downregulate the expression of ““““reticulocalbin 1 precursor““““, ““““nucleophosmin““““, ““““mitochondrial matrix protein pl precursor““““, and ““““stathmin““““. The changes in the expression of these genes suggested that TPA induced CNE2 cells to antiproliferation and to apoptosis, which was confirmed by subsequent apoptosis detection. Therefore, the effects of TPA on nasopharyngeal carcinoma cells were distinct from the effects on primary epithelial cells and we suggest reasons for these differences.     

基于MAXENT模型预测齿裂大戟在中国的潜在分布区

【背景】齿裂大戟原产于北美,是近年来入侵我国的一种检疫性杂草。目前,该杂草已在我国的华北、华东和西南建立种群,并呈扩散蔓延趋势。由于该入侵植物具有极强的繁殖能力,一旦大面积扩散势必造成极大损失,急需通过风险评估明确其未来的扩散趋势,进而制定早期预警措施。【方法】使用MAXNET模型,运用齿裂大戟在原产地和中国的已知分布数据及筛选后的环境变量,结合地理信息系统(Geographic information system,GIS)及其生活史特征和环境适应特性,直观和定量地预测了该原产北美的植物在中国的适生范围,并采用受试者工作曲线(Receiver operator characteristic curve,ROC)分析方法对模型预测结果进行了检验。【结果】齿裂大戟在我国有较为广阔的潜在分布区,其中高风险区主要集中在地处33°~40°N,109°~119°E的北京、天津、河北南部、河南北部、山东中北部、山西南部和陕西西安等地。【结论与意义】结合齿裂大戟在我国的分布现状和传入扩散特性,划定了其在我国潜在的高风险区域,为制定预防和控制入侵植物进一步传入和扩散的早期预警和监测措施提供科学依据。     

湖北大戟根中的环阿尔廷型三萜

从湖北神农架地区产湖北大戟(Euphorbia hylonomaHand.-Mazz.)根中分离得到4个环阿尔廷型三萜化合物,分别鉴定为:24-烯环阿尔廷软脂酸酯(1),环阿尔廷-23-烯-3β,25-二醇-3-乙酸酯(2),环阿尔廷-25-烯-3β,24ξ-二醇(3),环阿尔廷-23-烯-3β,25,28-三醇(4)。化合物1~4均为首次从该植物中得到。     

中国两种新归化植物——巴尔干大戟和假蒿

报道在云南发现的新归化植物,分别是大戟科巴尔干大戟(Euphorbia oblongata Griseb.)和菊科假蒿[Eupatorium capillifolium (Lam.) Small ex Porter&Britton],凭证标本均保存于云南大学植物标本馆(YUKU)。巴尔干大戟原产于欧洲南部和西亚,现发现归化于云南省昆明市呈贡区;巴尔干大戟的主要鉴别特征是幼茎和花序轴密被白色长柔毛,蒴果球形,有瘤状突起,无毛。假蒿原产于美国南部,是园艺上广泛栽培的物种,现发现其在云南省大理州剑川县逸野归化;假蒿的主要鉴别特征是植株被柔毛,叶片近长卵形,2至3回羽状全裂,小裂片线形或长圆形,头状花序排成复总状,每个头状花序有小花5,花冠白色微绿。文中描述巴尔干大戟和假蒿的形态特征,并提供两种的野外生态照片;此外,对其可能的传入途径进行分析,简要评估入侵风险与危害。     

药用植物红芽大戟的组织培养

针对药用植物红芽大戟结实率和种子萌发率较低的情况,探讨了利用组织培养方法解决生产中繁殖的问题。结果表明,在茎、叶和芽３种外植体中,芽的出愈率最高,愈伤组织褐化率低,生长最好,且易被诱导出不定芽,是较理想的快速繁殖材料;而茎和叶的愈伤组织褐化率高,难以诱导出不定芽。较适宜的诱导愈伤组织的激素组合是６－ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．４ｍｇ／Ｌ,诱导不定芽的适宜激素组合是６－ＢＡ５．０ｍｇ／Ｌ＋ＮＡＡ０．０５ｍｇ／Ｌ,根的诱导则在１／２ＭＳ＋ＮＡＡ０．２＋庶糖１．５％＋琼脂０．８％培养基上进行     

药用植物红芽大戟花的形态及种子繁殖特性的研究

本文研究红芽大戟花的形态,发现存在花柱异长现象。具有长花柱型花和短花柱型花两种类型。还研究了红芽大戟种子萌发的过程及幼苗生长情况。用２５ｐｐｍ赤霉酸（ＧＡ３）浸种处理长花柱型花结的种子,用５０ｐｐｍＧＡ３浸种处理短花柱型花结的种子,可以较好地提高种子的萌发率,其种子萌发率分别达７０．２％和６４．１％。幼苗的生长以弱酸性的砂壤土更为适宜     

大戟内生菌的抑菌活性及其与宿主相关性研究

为了开发利用药用植物的内生菌资源,以大戟和其内生真菌作为材料,以一株乌桕来源的内生菌作为对照,研究自身具有抑菌作用的药用植物的内生菌在抑菌作用上和宿主的相关性.结果表明,大戟内生菌镰刀菌E5有抑制宿主受试病原菌的作用,并在活植物体内表现出对受试病原菌的拮抗作用;其抑菌能力受宿主成分刺激而加强,而宿主成分对受试病原菌无抑制作用,乌桕来源的内生菌链隔孢S12无抑制受试病原菌的作用.内生菌E5需要在氧气充足条件下培养才能产生抑菌作用,抑菌活性最大产生时间和生物量最大值不同时出现.内生菌E5的抑菌能力受到pH、温度、紫外线影响.大戟内生菌E以上特点,体现了其与宿主之间的互惠共生关系.     

Comparative Studies on the Allelopathic Effects of Ulva pertusa Kjellml, Corllina pilulifera Postl et Ruprl, and Sargassum thunbergii mertl O. Kuntze on Skeletonema costatum （Grev.）Cleve

In the present study, we evaluated the allelopathic effects of three macroalgae, namely Ulva pertusa Kjellml, Corallina pilufifera Postl et Ruprl, and Sargassum thunbergii Mertl O. Kuntze, on the growth of the microalga Skeletonema costaturn （Grev.） Creve using culture systems in which the algae coexisted. The effects of the macroalgal culture medium filtrate on S. costatum were also investigated. Moreover, isolated co-culture systems were built to confirm the existence of allelochemicals and preclude growth inhibition by direct contact. The coexistence assay data demonstrated that the growth of S. costaturn was strongly inhibited when fresh tissues, dry powder and aqueous extracts were used; the allelochemicals were lethal to S. costatum at relatively higher concentrations. The effects of the macroalgal culture medium filtrate on the microalga showed both species specificity and complexity. The inhibitory effect of fresh macroalgal tissue and culture medium filtrate on the microalga was due to the alleochemicals released by the macroalgae. The results of the present study show that the allelopathic effects of macroalgae on the microalga are complex. The present study could shed light onto the basis of the interaction between macro- and microalgae.     

大果大戟中的一个对映-贝壳杉烷型二萜

从大果大戟的根部首次分离得到一个对映-贝壳杉烷型二萜,利用波谱方法鉴定为ent-16α,17-dihydrox-ykauran-3-one(1)。首次对化合物1在甲醇中的碳谱和氢谱数据进行了全归属。     

福建产1种中国大陆新记录归化植物

报道了采自福建省厦门市的1种中国大陆新记录归化植物——匍根大戟(Euphorbia serpens H.B.K.),标本存放于华南农业大学林学院植物标本室(CANT).