用免疫荧光单克隆抗体对脊髓灰质炎病毒抗原表位的分析

用间接免疫荧光与中和试验筛选出来的抗脊髓灰质炎2型与3型不同毒株的12个单克隆抗体,其中3个仅有免疫荧光活性,9个具有中和与免疫荧光活性。用免疫荧光活性的单克隆抗体进行试验,发现它们在识别特异性抗原表位方面与中和性单克隆抗体相似,显示出株特异的、几个毒株共同特异的或型特异的抗原表位。根据表位分布关系及特征,可以用来鉴别型内毒株的特征、毒株的抗原分析、抗原变异的研究以及疫苗相关病例的鉴别。所得结果与中和性单克抗隆抗体及T1-寡核苷酸指纹图谱分析一致。而免疫荧光单克隆抗体识别抗原表位的活性范围比中和性单克隆抗体更广。另外还发现某些兼有荧光与中和两活性的同一个单克隆抗体,用不同方法(IF与NT)进行试验时,与相同毒株出现不同表位反应,这点是值得引起注意需待进一步证实的重要问题。     

从我国成人手足口病患者疱液中首次分离出肠道病毒71型

本文报道了从我国手足口病(HFMD)患者疱液中肠道病毒71(E71)型H株的分离和鉴定。该株病毒可在原代人胚肺(HEL)细胞、MA104细胞、BSC细胞中生长繁殖,导致典型的肠道病毒CPE出现。将H株接种乳鼠后出现肢体麻痹、第6天开始死亡。电镜下可见感染H株的BSC细胞胞浆中出现大量的结晶状排列的成熟病毒颗粒,直径约25nm。患者双份血清中有对H株4倍增高的中和抗体存在。采用100抗体单位的抗Cox A5、7、9、16、E70、E71抗体和50抗体单位的LBM组合血清A-H以及抗E71BrCr株MeAb P27对H株进行中和试验时,H株可被抗E71血清和MeAb P27所中和。抗E71抗体对H株的最低有效中和作用为1.6抗体单位,MeAb P27对H株的有效中和作用是64抗体单位。其它血清则无此中和作用。然而,在鉴定过程中发现,高滴度的抗Cox A16抗体(200抗体单位以上)也显出有中和H株的作用,提示我们所分离的H株含有与Cox A16的型间共同抗原。     

巨细胞病毒冻干保存

The preservation of cytomegalovirus (CMV) by lyophilization was first developed in China. In 4 to 10 days the typical CPE was up to + in the HEL cell line which was inoculated only 0.2 ml suspension of lyophilized strain AD169 or CMV isolated from milk breast. After preserved for 1 year the lyophilized cytomegalovirus strain still contained 1.5-2.0 lgTCID50/0.1 ml in titer of viral infection. The lyophilized strains have been used in the preparation of diagnostic antigen of cytomegalovirus in our department, also the strains were supplied to the whole country.     

菜粉蝶颗粒体病毒对哺乳动物的实验感染

The experimental infection of mammals (such as mouse, golden hamster and nude mouse) was conducted with Pieris rapae Granulosis Virus (PrGV) of baculoviridae of insect virus by way of peritonal and intravenous injection, per os and inhalation. 7-50 days after injection, target insects were reinoculated with the visceral extracts of infected mammals and killed by 5-100%, displaying typical symptom infected by granulosis virus. GV and its latticed structure of inclusion body were found in the ultrathin section of spleen which took out from infected nude mouse via peritoneal injection under electronmicroscope.     

在被小泡参与突触前后膜特化增厚的形态学研究

Our object was to characterize the morphological changes of coated vesicles and synaptic membranes during synaptogenesis. Neurons from spinal cords of fetal mice were established as isolated cells in primary culture. After a few days in vitro, the neurons extended their neurites and started their interaction. At timed intervals thereafter, cultures were fixed for electron microscopic observation. Coated vesicles were prominent in the neuronal cytoplasm at the time of synaptogenesis (about 7-10 days in vitro). Similar vesicles were seen in continuity with some cisternae in the Golgi regions and there was an increase in number during the synaptogenic period. Indeed it is not established whether the coated vesicles were exocytotic or pinocytotic in nature, but the cisternae which were in continuity with coated vesicles could be labelled by glucose-6-phosphatase (G6Pase) but not by thiamine pyrophosphatase (TPPase). Such vesicles were also seen in continuity with the neuronal plasmalemma near the closest contact site and contributed their undercoating to pre- and postsynaptic densities. The formation of bilateral membrane specialization was described as being structurally similar to synaptic active zones and appeared to be the first definitive sign of synapseformation. It has been suggested that the synaptic dense material may derive wholly or in part from the exocytic coated vesicles which apparently budding off from endoplasmic reticulum cisternae. This incorporation could provide the mechanism for confining specific characteristics of neuronal membrane to the synaptic region.     

相分配—一种纯化细胞器和膜微囊的方法

根据各种膜颗粒表面电荷密度和疏水性的差异,通过适当的相分配系统,将一种膜颗粒从其它成份中分离出来,这便是相分配法。在相分配法应用之前,都是用离心法纯化膜颗粒的,但离心法纯化的膜颗粒纯度不够高。相分配法作为离心法的辅助技术,则能满足实验对分离纯化的膜颗粒的纯度     

干旱对大豆线粒体膜脂的磷脂和脂肪酸组分的影响

干旱胁迫致使大豆叶片线粒体膜脂的PG摩尔百分含量降低,而LPC,PC,PI,PE,PA则明显提高。抗旱品种和敏感品种之间棕榈酸及亚麻酸含量有明显差异,且抗旱品种叶片线粒体膜脂的总脂肪酸不饱和指数低于敏感品种。     

用聚合酶链式反应法检测人乳头状瘤病毒DNA

实验研究表明,宫颈癌的发生和发展与人乳头状瘤病毒(Human papillomavirus,HPV)的感染有着密切关系。约有80％的宫颈癌组织中含有人乳头状瘤病毒第16型(HPV 16)及18型(HPV 18)的DNA,因此,检测宫颈癌组织中的HPV DNA对研究宫颈癌的发生和发展机制以及宫颈癌的防治都有重要意义。目前检测HPV DNA的手段都采用DNA探针杂交技术,该方法要有高比活的同位素,而且费时。聚合酶链式反应(Polymerase Chain Rea-     

兔的一种新病毒：Ⅱ.一株兔出血症...

In this paper a strain of Rabbit Hemorrhagic Disease Virus (RHDV) was isolated and purified from the diseased rabbit livers with a method of using chloroform, two-phase of polyethylene-glycol-dextran sulfate sodium and sucrose density gradient centrifugation. Purified virus was nonenveloped, icosahedeal symmetry with a triangulation number of 3, and 33-37 nm in diameter. The capsid was composed of 32 capsomeres with central holes in an outer diameter of about 9nm. Two types of viral particles having different sedimentation coefficient, 130s and 166s could be identified after sucrose density gradient centrifugation. Probably no less than four virion proteins with molecular weight of 66.4, 65.0, 63.5, 41.0 x 10(3) dalton were detected by SDS-polyacrylamide gel electrophoresis. Viral nucleic acid was extracted from purified virus by using SDS-proteinase K-phenol. Tests with diphenylamine, formaldehyde, and staining with acridine orange as well as the curves of thermal denaturation showed that this kind of virus had a single-stranded DNA. The molecular weight of the ssDNA was approx 2.1 x 10(6) dalton as determined by electron microscopy. Data indicate that the RHDV may like the parvovirus of the family Parvoviridae.     

脱氧血红蛋白与红细胞膜的相互作用

人红细胞中血红蛋白主要以游离态存在,也有小部分与膜结合。文献报道,脱氧状态下两者相互作用加强,我们研究了这种相互作用的生物学意义,主要测定了脱氧状态下:(1)红细胞的低渗溶血速率;(2)红细胞膜对阴离子的通透性;(3)红细胞的变形性。