Promotive Effect of Low Concentrations of NaHSO3 on Photophosphorylation and Photosynthesis in Phosphoenolpyruvate Carboxylase Transgenic Rice Leaves

Spraying a 1-2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.)Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC phosphate dikinase(PPDK) transgenic rice (PC PK), in which the germplasm was transformed with wild-type Kitaake as the gene receptor, resulted in an enhancement of the net photosynthetic rate by 23.0%, 28.8%, and 34.4%,respectively, for more than 3 d. It was also observed that NaHSO3 application caused an increase in the ATP content in leaves. Spraying PMS (a cofactor catalysing the photophosphorylation cycle) and NaHSO3 separately or together on leaves resulted in an increase in photosynthesis with all treatments. There was no additional effect on photosynthetic rate when the mixture was applied, suggesting that the mechanism by which NaHSO3 promotes photosynthesis is similar to the mechanism by which PMS acts and that both of compounds enhanced the supply of ATE After spraying a solution of NaHSO3 on leaves, compared with the WT Kitaake rice, a greater enhancement of net photosynthetic rate was observed in PEPC transgenic(PC) and PEPC PPDK transgenic (PC PK) rice, with the greatest increase being observed in the latter group. Therefore ATP supply may become the limiting factor that concentrates CO2 in rice leaves transformed with an exogenous PEPC gene and exogenous PEPC PPDK genes.     

含硫氰酸根离子的乳过氧化物酶-I--H2O2系统对Pg和Fn生长的影响

目的研究含生理浓度硫氰酸根离子(SCN-)的乳过氧化物酶(LP)-I--H2O2抗菌系统对牙龈卟啉单胞菌(Pg)和具核梭杆菌(Fn)生长的影响.方法 Pg和Fn各分5组每组含Pg(6.0×108/ml)或Fn(1.0×108/ml)菌悬液、含不同浓度I-的LP-I-系统、SCN,在37 C震荡水浴分别培养0 min(加H2O2前)和30min,5μl DTT终止反应,10倍浓度系列稀释,接种于BHI-S琼脂培养基,厌氧培养4 d,并计数CFU.结果 I-浓度增加至大于等于500 μmol/L时,LP-I-抗菌系统抑菌作用明显高于单独的LP-SCN--系统(P＜0.05).结论生理浓度SCN-存在时,通过增加I-的浓度可达到显著提高LP-I-抗菌系统抑制Pg和Fn生长的作用.     

异位妊娠破裂失血性休克的抢救及护理

异位妊娠破裂失血性休克病情发展凶、险、急,如不及时有效抢救,休克继续发展,将造成生命危险.我科自2001年1月至2003年12月共收治异位妊娠破裂失血性休克患者156例,由于抢救及时,护理周到,无1例发生意外,平均住院天数为8.75天,均为治愈出院.现将抢救及护理体会总结如下.     

稻米品质性状对开放式空气二氧化碳浓度增高的响应

利用开放式空气CO2浓度增高(FACE)系统平台。研究大田栽培条件下粳稻武香粳14号稻米品质性状对CO2浓度增高200μmol·mol^-1的响应。结果表明．FACE处理稻谷的出糙率平均比CK高1．4个百分点,整精米率平均比CK低12．3个百分点,较低的供N水平有利于提高FACE条件下的出糙率．较高的供N水平有利于提高FACE条件下的整精米率;FACE处理的稻米垩白略有增加。垩白粒率平均比CK高11．9个百分点,垩白度平均比CK平均高2．8个百分点,较高的供N和供P水平有利于降低FACE条件下垩白大小、垩白粒率和垩白度;FACE处理稻米糊化温度平均比CK平均高0．52℃,胶稠度有提高的趋势,但对稻米直链淀粉含量影响较小,较高的供N和供P水平有利于降低FACE条件下稻米的直链淀粉含量,较低的供N和较高的供P水平有利于降低FACE条件下稻米胶稠度,较低的供N水平有利于降低FACE条件下稻米糊化温度;FACE处理使稻米蛋白质含量比CK平均低0．6个百分点,较低的供N和供P水平有利于降低FACE条件下稻米蛋白质含量。     

半胱胺盐酸盐减缓山羊手术后细胞免疫抑制效应的实验研究

Sixteen castrated male goats were randomly allocated into two groups (Control n=8; Experiment n=8) to investigate the effects of cysteamine on surgery-induced cellular immune suppression. The experiment commenced with 13 d of pre-operation (pre-Op), which contained 3 d of roughage-fed period (RP) and 10 d of roughage + concentrate-fed period (RCP), followed by first operation (Op-1) and then the second operation (Op-2). The goats were allowed to have 14 d of recovery between two operations. The fistulas were fixed to rumen walls in Op-1 and to duodenal walls in Op-2. Cysteamine (15 mg x kg(-1) x BW x d(-1)) was added to diet and fed to goats in Exp. group since RCP of pre-Op through whole experimental period. Blood was sampled through jugular vein from goats of both groups at pre-feeding of the last day of RP and RCP, 8 d after Op-1, 4 d and 13 d after Op-2, respectively to determine serum concentration of interlukin-2 (IL-2), cortisol, as well as PHA-stimulated lymphocytic proliferation rate (SI). In pre-operative periods there were no significant differences in concentration of IL-2 (0.82+/-0.1 vs 1.03+/-0.22 microg l(-1)) and cortisol (20.48+/-4.52 vs 20.80+/-9.93 microg l(-1)), as well as lymphocytic SI (90.72+/-7.25 vs 129.22+/-18.59) between Control and Exp. groups. The concentrations of IL-2 (0.82+/-0.1 vs 0.73+/-0.08 vs 0.55+/-0.12 microg l(-1)) and lymphocytic SI (90.72+/-7.25 vs 49.31+/-6.4 vs 7.09+/-1.66) in Control were depressed acutely (P<0.05), but cortisol concentration elevated (20.48+/-4.52 vs 26.67+/-10.51 vs 32.33+/-3.29 microg l(-1)) in post-operative periods (8 d after Op-1 and 4 d after Op-2), compared with those in RCP. While they remained unaltered in Exp. group except a slight decrease of lymphocytic SI at 4 d after Op-2. The different responses of Control and Exp. groups to surgery stress led to a 80-90% higher of IL-2 and about 3 times greater of SI in Exp. than those in Control, which was contributed by cysteamine administration. Our data show that in operated goats the cellular immune was suppressed by surgery stress, cysteamine administration prevented decreases of IL-2 and lymphocytic SI and increase of cortisol induced by surgery stress, consequently attenuates surgery-induced suppression of cellular immune activity.     

不同土壤水分条件下CO2浓度对禾谷缢管蚜种群的影响

利用开顶式熏气室研究了不同土壤水分条件下不同CO2浓度对禾谷缢管蚜种群的影响,以期对未来大气CO2浓度升高条件下不同降雨地区的小麦－蚜虫关系发展趋势做出蚜虫关系发展趋势做出初步预测,结果表明,随CO2浓度从350ul.L^-1上升至550ul.L^-1时,60％土壤水分下的种群增长最快;当CO2浓度从550ul.L^-1上升到700ul.L^-1时,60％和40％土亍水分下的种群增长相近,且高于80％土壤水分下的增长,据此可以认为,随大气CO2浓度升高,禾谷缢管蚜种群会持续增长,从目前至下世纪中叶的时间内可能是蚜种群增长最快的阶段,特别在干旱,半干旱地区禾谷缢管蚜种群增长幅度较大,小麦受受较重。     

不同土壤水分条件下CO_2浓度对禾谷缢管蚜种群的影响

利用开顶式熏气室研究了不同土壤水分条件下不同CO2 浓度对禾谷缢管蚜种群的影响 ,以期对未来大气CO2 浓度升高条件下不同降雨地区的小麦 蚜虫关系发展趋势做出初步预测 .结果表明 ,随CO2 浓度升高 ,禾谷缢管蚜种群持续增长 ,但以CO2 浓度从 35 0 μl·L-1上升到 5 5 0 μl·L-1时增长最快 ;禾谷缢管蚜种群大小与土壤水分密切相关 ,各CO2 浓度下均以 6 0 %土壤水分的最大 ;当CO2 浓度从 35 0 μl·L-1上升到 5 5 0 μl·L-1时 ,6 0 %土壤水分下的种群增长最快 ;当CO2 浓度从 5 5 0 μl·L-1上升到 70 0 μl·L-1时 ,6 0 %和 40 %土壤水分下的种群增长相近 ,且高于 80 %土壤水分下的增长 .据此可以认为 ,随大气CO2 浓度升高 ,禾谷缢管蚜种群会持续增长 ,从目前至下世纪中叶的时间内可能是蚜虫种群增长最快的阶段 ,特别在干旱、半干旱地区禾谷缢管蚜种群增长幅度较大、小麦受害较重 .     

硒对NO诱导的内皮细胞内游离钙离子浓度变化的影响

用Fura-2显微荧光测钙技术,研究了用外源性一氧化氮（NO）供体S-亚硝基谷胱甘肽（GSNO）诱导的,人脐静脉内皮细胞系ECV-304细胞胞内游离钙离子浓度（［Ca²⁺］_i ）升高以及硒的抑制效应.结果表明,GSNO作用于ECV-304细胞,短时间内即可导致其胞内游离钙离子浓度升高.胞外液换为无钙液或向胞外液中加入CdCl₂（1 mmol/L）对GSNO引起的［Ca²⁺］_i升高无影响.提示,GSNO刺激主要引起胞内钙库释放.而且,一氧化氮清除剂血红蛋白（Hb）对这一过程有抑制作用,说明GSNO引起的胞内钙库释放由NO介导.经亚硒酸钠（1 μmol/L）处理的细胞,其NO引起的［Ca²⁺］_i升高幅度明显被抑制,说明NO的这种作用可能与细胞的氧化还原状态有关.     

开放式空气CO2浓度增高对水稻N素吸收利用的影响

在大田栽培条件下,研究空气中CO₂浓度增高(FACE)200μmol·mol^-1对水稻N素吸收及其利用效率的影响.结果表明,FACE处理使水稻不同生育时期的植株含N率显著下降;由于干物质生产量显著增大,FACE处理使水稻不同生育时期的N素累积量有所提高,但无显著影响;FACE处理能够显著提高移栽后28d、抽穗期以及成熟期单位N素的干物质生产效率、单位N素的籽粒生产效率和显著提高水稻的N素收获指数.高N处理的植株含N率、N素累积量均有所增加,但使N素生产效率呈现下降趋势.