人类T细胞白血病病毒I型pX基因反式调节和致白血病机理的研究进展

人类Ｔ细胞白血病病毒Ｉ型（ＨＴＬＶ－Ｉ）是成人Ｔ细胞白血病（ＡＴＬ）的病因。ＨＴＬＶ－Ｉ基因组中的ｐＸ基因区域编码ｐ４０＾ｔａｘ和ｐ２７＾ｒｅｘ蛋白,前者可反式激活病毒的ＬＴＲ和ＩＬ－２Ｒ及ｃ－ｆｏｓ、ＴＧＦβ１等细胞基因,加强转录过程,在白血病发生中发挥重要作用。ｐ２７＾ｒｅｘ蛋白则在转录后水平进行反馈性调节。白血病的发生机理是多因子参加的多步骤过程。     

大鼠酷氨酸羟化酶基因在肌母细胞中稳定表达

用电穿孔法将大鼠酷氨酸羟化酶（Ｔｙｒｏｓｉｎｅ ｈｙｄｒｏｘｙｌａｓｅ,ＴＨ）基因转染大鼠Ｌ６ＴＧ成肌细胞株,经ＰＣＲ检测、免疫组织化学和荧光组织化学检测证明,ＴＨ基因能在细胞内稳定整合和表达,并在辅因子存在时将酷氨酸转化为多巴,移植于大鼠纹状体后可成活并表达ＴＨ。     

汉滩病毒核壳蛋白（NP）在杆状病毒系统的表达及其免疫原性研究

应用杆状病毒表达载体成功地表达了汉滩病毒７６－１１８株（ＨＴＮＶ）核壳蛋白,将ＨＴＮＶＳ基因插入杆状病毒转染质粒ｐＡｃＹＭＩＢ的多角体基因启动子下游附近,与经Ｂｓｕ３６１酶切线性化的杆状病毒（ＡｃＶＥＰＡ）ＤＮＡ共同转染Ｓ１９细胞,经空斑筛选获得了高效表达ＮＰ的重组杆状病毒（ＡｃＶＨａｎＳ）。经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ证实,表达产物与ＨＴＮＶ毒粒ＮＰ分子量均为５０ＫＤ左右,紫外扫     

丙型肝炎病毒基因组部份NS5区蛋白基因在大肠杆菌中的表达

在大肠杆菌中表达了丙型肝炎病毒基因组ＮＳ５区Ａ段和部份Ｂ段蛋白。ＮＳ５Ａ蛋白溶于水,可经过ＧＳＴ亲和层析柱纯化;ＮＳ５Ｂ蛋白不溶于水,经ＰＢＳ－Ｔｒｉｔｏｎ Ｘ－１００洗涤,尿素溶解后,通过离子交换柱纯化。经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｍｂｌｏｔ分析,ＮＳ５Ａ蛋白除在５７ｋＤ左右有条带外,还有不同程度的降解产物;ＮＳ５Ｂ蛋白主要在５８ｋＤ左右有条带出现。为查明表达蛋白抗体在病人血清中的分布,取９     

鸡AChRγ亚基基因远端E盒子与myogenin的相互作用

业已证明,鸡ｎＡｃｈＲγ亚基基因５连接区,－５０９／－３０２,－３２４／＋３６片段可独立激活异源启动子ＳＶ４０的转录活性,γ基因近端两个Ｅ盒子（ＣＡＮＮＴＧ）与生肌调节因子（ｍｙｏｇｅｎｉｎ）的相互作用及转录激活分析表明,近端两个Ｅ盒子是γ启动子活性必不可少的,但却是不充分的,利用胶阻滞和ＤＮａｓｅＩ足级试验观察了鸡ｎＡｃｈＲγ基因远端Ｅ盒子与ＧＳＴ－ｍｙｏｇｅｎｉｎ融合蛋白的相互作用,胶阻滞试验     

Physical mapping of three fruit ripening genes：Endopolygalacturonase,ACC oxidase and ACC synthase from apple（Malus x domestica） in an apple rootstock A106（Malus sieboldii

The apple rootstock,A106(Malus sieboldii),had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell.Karyotypes were prepared from root-tip cells with 2n=34 chromosomes,Seven out of 82 karyotypes(8.5%) showed one pari of satellites at the end of the short arm of chromosome 3.C-bands were shown on 6 pairs of chromosomes 2,4,6,8,14,and 16 near the telomeric regions of short arms.Probes for three ripening-related genes from Malus x domestica:endopolygalacturonase(EPG,0.6kb),ACC oxidase(1.2kb),and ACC synthase(2kb)were hybridized in situ to metaphase chromosomes of A106.Hybridization sites for the EPG gene were observed on the long arm of chromosome 14 in 15 out of 16 replicate spreads and proximal to the centromere of chromosomes 6 and 11.For the ACC oxidase gene,hylridization sites were observed in the telomeric region of the short arm of chromosomes 5 and 11 in 87% and 81% of 16 spreads respectively,proxiaml to the centromere of chromosome 1 in 81% of the spreads,and on the long arm of chromosome 13 in 50% of the spreads. Physical mapping of three fruit ripening genes in an apple rootstock A106.Twenty five spreads were studied for the ACC synthase gene and hybridization sites were observed in the telomeric region of the short arm of chromosome 12 in 96% of the spreads.chromosomes 9 and 10 in 76% of the spreads,and chromosome 17 in 56% of the spreads.     

Short Communication—The detection of nuclear matrix in most primitive presentexisting eukaryote,Giardia lamblia

The nuclear matrix of diplomonad Giardia lamblia was detected for the first time with DGD embedmentsectioning-embedment free electron microscopy after a series of specific extractions.The result showed that archaezoa Giardia Lamblia already possessed nuclear matrix within its two nuclei.The finest fibrils of the nuclear matrix of Giardia lamblia were measured to be about 11 to 13 nm in thickness.However,the nuclear lamina and nucleolus have never been observed.These results seem to suggest that nuclear matrix is an indispensable intranuclear structural component even in the primitive nucleus.     

肾脏带3蛋白研究进展

肾脏带3蛋白介导的HCO3－／Cl-交换在肾小管酸碱调节中起重要作用,近年对其在肾小管中的定位,结构,基因组成和功能的研究极为活跃,本对近年的研究进展作一综述。     

肿瘤抑制基因研究新进展：多种瘤抑制基因MTS分子生…

人类第９号染色体短臂不到４０ｋｂ的座位发现了多种瘤抑制基因。它们分别编码两种蛋白：前叫做Ｐ１６蛋白,抑制依赖细胞周期素激酶。后比Ｐ１６小２０多个残基,功能推测与Ｐ１６相近。ＭＴＳ１基因与Ｐ５３相比,它在７５％的各种癌细胞中发生缺失;而Ｐ５３基因的缺失率在各种癌细胞中为５０％。Ｐ１６蛋白是到目前为止发现的第一个直接控制细胞增殖周期的细胞固有蛋白,而Ｐ５３控制细胞增殖周期要通过Ｐ２１间接发挥抑制作