Advancement in studies of tree growth and ecophysiology incorporating micro-sampling approach

The recently developed micro-sampling approach has been widely used to extract micro-tree-cores at weekly intervals to monitor the process of stem cambial activity and xylem formation. Compared with the traditional dendrochronology, the micro-sampling approach enables us to better understand the inherent physiological processes in tree growth and their relationships with the environment at a more precise level. This review article aims to: 1) summarize the progresses in the micro-sampling approach-based studies published over recent years and its potential applications, and 2) elucidate the relationships between primary growth and secondary growth and the response mechanisms of radial growth of trees to global change (global warming, drought, and carbon and nitrogen fertilization effects) based on information from literature. It is anticipated that this review will assist with predicting productivity and carbon sink potential of forests, and help policy-makers with sustainable forest management decisions.     

Box-Behnken设计-响应面法优化人参干细胞培养体系

[目的]研究人参形成层干细胞(Cambial Meristematic Cell, CMCs)培养体系的构建及相关工艺优化。[方法]通过Box-Behnken设计-响应面法优化人参CMCs培养相关工艺。[结果]从人参形成层中分离得到人参形成层细胞CMCs,诱导分离的最佳培养条件为：B5+IAA 6 mg/L+IBA 6 mg/L+蔗糖20 g/L+琼脂粉7 g/L+L-抗坏血酸100 mg/L+柠檬酸150 mg/L,诱导率为50.54%;固体增殖的最佳培养条件为：B5+IAA 2.9 mg/L+IBA 2.6 mg/L+NAA 5.9 mg/L+蔗糖20 g/L+L-抗坏血酸100 mg/L+柠檬酸150 mg/L,增殖量为1.01 g;悬浮培养的最佳培养条件为：B5+IAA 5 mg/L+IBA 5 mg/L+NAA 5 mg/L+蔗糖20 g/L+L-抗坏血酸100 mg/L+柠檬酸150 mg/L,pH 6.2,增殖量为3.05 g;均为24.6℃暗培养。[结论]人参CMCs具备干细胞特征，Box-Behnken设计-响应面法适用于人参CMCs培养工艺的优化。