荔波野生宜昌橙

一九八一年在荔波县果树资源调查中,发现玉屏区时来乡石灰砌村水架村民组拉哑湾森林内零星和连片分布。群众称为野桔,经鉴定为野生宜昌橙,当地群众采集入药,近几年来用于甜桔育苗砧木。宜昌澄(Citrus ichangensis),别名:宜昌柑、罗汉柑,当地土名叫野桔。属于蟹橙类的一种。植物形态:为常绿多刺小乔木或灌木。树高1.5～4米;主杆皮色为灰绿色,征枝干上有坚锐长刺,幼技有棱角及短刺,刺长1.5～3厘米,结果枝常无刺或少刺。叶片卵圆形或长卵圆形,先端渐尖,基部圆形,长3—6.5厘米,宽1.5～3.5厘米;叶柄长,有宽大     

无根根零R25和普通变形杆菌P1混合培养发酵L-苹果酸的研究

所筛的41株属于根霉属的菌株,均具有从葡萄糖产生延胡索酸的能力。其中,R25产延胡索酸能力最高,同时产生部分L-苹果酸。该菌株被鉴定为无根根霉(Rhizopus arrhizus)。普通变形杆菌(Protcus vulgaris) Pl具有强的延胡索酸酶活性：可将延胡索酸转化为L一苹果酸。     

油菜田三突花蛛的生物学特性初报

通过大田观察和室内饲养,发现武汉地区油菜田三突花蛛3月中旬开始出来活动,4月中旬达到高峰期,主要隐蔽于曲菜花丛中,以待猎物到来,不结网,有飞航习性,数量达225头/百株,占总捕食性天敌的82％,同时开始交配、产卵。不仅与蚜虫、叶蝉的发生正相关,也和油菜的长势呈现正相关性,并对害虫起到明显的控制作用。可见,是油菜田害虫的重要天敌。     

中国林蛙地模标本染色体组型、C带和银带研究

本文报道中国林蛙地模标本的染色体组型、C带和银带。选用染色单体未分开的早中期分裂相制作C带可显示出较多带纹。研究结果表明,中国林蛙与亲缘关系较近的欧洲林蛙在染色体组型、C带和银带上均存在显著差异。再染色体进化上,欧洲林蛙比较原始,中国林蛙比较进化。     

小鼠巨核系祖细胞增殖,分化特性的研究

小鼠骨髓细胞经7d培养后进行细胞形态学观察,可见不同发育阶段的巨核细胞及不同大小的巨核细胞集落。通过计数每个集落中的细胞数,可确定相应祖细胞的有丝分裂能力。结果表明,具有不同有丝分裂能力的祖细胞的体外增殖动力学有所不同。祖细胞的数量与其有丝分裂次数呈负相关(r=-0.986)。进行0、1、2和3次有丝分裂的祖细胞的阿糖胞苷自杀率分别为48.9,58.7,48.0和41.2％;放射敏感性的D_O值(Gy)分别为1.71,1.24,1.03和0.77,D_O值的大小与有丝分裂次数呈负相关(r=-0.958)。经3Gy全身照射后CFU-Meg与CFU-GM的恢复动态过程具有不同特点。     

Construction and packaging of pseudotype retrovirus containing human N—ras cDNA antisense sequence and its biological effects on human hepatoma cells

N-ras is one of the transforming genes in human hepatic cancer cells.It has been found that N-ras was overexpressed at the mRNA and protein level in hepatoma cells.In order to explore the biological roles of N-ras in human hepatic carcinogenesis and the potential application in control of cancer cell growth,a preudotype retrovirus containing antisense sequence of human N-ras was constructed and packaged.A recombinant retrovirus vector containing antisense or sense sequences of N-ras cDNA was constructed by pZIP-NeoSV(X)1.The pseudotype virus was packaged ang rescued by transfection and infection in PA317 and ψ 2 helper cells.It has been demonstrated that the pseudotype retrovirus containing antisense N-ras sequence did inhibit the growth of human PLC/PRF/5 hepatoma cells accompanied with inhibition of p21 expression,while the retrovirus containing sense sequence had none.The pseudotype virus had no effect on human diploid fibroblasts.