昆虫与植物关系的研究进展和前景

本文综述昆虫与植物之间关系的研究概况，包括历史渊源、昆虫选择寄主植物的生理机制，植物对虫害的反应、用抗虫基因在作物中移植以防治害虫和展望。着重叙述昆虫神经中枢对于植物理化特性所产生的感觉内导的综合作用，植物蒙受虫害后的补偿作用及由此诱导所产生的化学防御作用。讨论了以抗虫基因移植于农林作物来防治害虫是否会引起昆虫对这种新育成的植物产生适应或抗性。昆虫与植物之间的关系是一个重要的科研领域，对其发展前景     

Human μ—opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi／0 proteins

Human mu-opioid receptor (HmuOR) with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect cells. The maximal binding capacity for the [3H] diprenorphine and [3H]ohmefentanyl (Ohm) were 9.1 +/- 0.7 and 6.52 +/- 0.23 nmol/g protein, respectively. The [3H] diprenorphine or [3H] Ohm binding to the receptor expressed in Sf9 cells was strongly inhibited by mu-selective agonists [D-Ala2, N-methyl-Phe4, glyol5]enkephalin (DAGO), Ohm, and morphine, but neither by delta nor by kappa selective agonist. Na+ (100 mM) and GTP (50 microM) could reduce HmuOR agonists etorphine and Ohm affinity binding to the overexpressed HmuOR. mu-selective agonists DAGO and Ohm effectively stimulated [35S]GTP-gammaS binding (EC50 = 2.7 nM and 6.9 nM) and inhibited forskolin- stimulated cAMP accumulation (IC50 = 0.9 nM and 0.3 nM). The agonist-dependent effects could be blocked by opioid antagonist naloxone or by pretreatment of cells with pertussis toxin (PTX). These results demonstrated that HmuOR overexpressed in Sf9 insect cells functionally coupled to endogenous G(i/o) proteins.     

鼠白细胞介素12（mIL—12）在昆虫细胞中的表达

Since human IL-12 is species-specific in its functions and elicits little biological responses from mouse lymphocytes, it is necessary to express recombinant murine IL-12 for the usage in studying the effects of this cytokine in various rodent models. Thereby, we can investigate the role of IL-12 in immune response in vivo and evaluate its potential clinical utility. Thus, we firstly constructed two expression vectors, pVL1393-mp40 and pVL1393-mp35. They were used to co-transfect the insect cells(Sf9) separately with linearized polyhedrosis virus genomic DNA. Two kinds of recombinant viruses AcNPV-mp40 and AcNPV-mp35 were visually screened out, and mp40 and mp35 were co-expressed in the insect cells co-infected by AcNPV-mp40 and AcNPV-mp35. The results of real-time Biomolecular Interaction Analysis (BIA) and Northern blot demonstrated that the recombinant mIL-12 was expressed successfully in the insect cells. The molecular weights of recombinant mp40 and mp35 were 40 KDa and 22 KDa on SDS-PAGE under reducing conditions, respectively. The apparent molecular weight of recombinant mIL-12 is 80 KDa under non-reducing conditions of Western blot. Biological activity of the recombinant product was detected in conditional medium using antibody-capture bioassay. The expression level of recombinant mIL-12 was about 10-15 micrograms/10(6) cells, as compared with the calibration curve of mIL-12.     

昆虫幼虫储存蛋白

本文对昆虫幼虫血淋巴内一种特殊性质的蛋白－幼虫储存蛋白的命名、生化特性、合成部位、生理作用和体内脂肪体合成、贮存和利用的调节机制等方法的研究进展进行了综述。     

开发昆虫新资源──浅谈白蚁的搜集

昆虫资源开发是近几年来研究的一项新课题,昆虫学家充分肯定了部分昆虫体内含有多种营养物质,并应加以开发。目前,对昆虫资源开发的文章时有发表,但对昆虫资源搜集方面的情况从未见过报道,现将如何搜集白蚁和进行初步加工的方法整理如下。1家白蚁Coptotermesspp．的搜集白蚁是社会性昆虫,行动诡秘,破坏性惊人,特别是家白蚁对建筑物的破坏更大,一旦发现家白蚁,往往会造成人心惶惶,令人深恶痛绝。家白蚁习惯将蚁巢建于大树底部和土木交接处,质地结实,这为家白蚁搜集提供了定位巢穴的线索。家白蚁蚁巢一经发现,迅速将蚁巢连同白…     

我国食用昆虫的开发简况

世界各地许多民族有食虫的习俗,我国早在周朝就有食虫的文字记载。但人们食用昆虫的量不多,亦不普遍,其手段主要是捕集野生昆虫为主。现今,为有计划地开发昆虫资源,进行了大量的科技投人,某些高等院校及研究单位建立了相应的研究机构,立题攻关,取得了可喜成果,并面向市场,走科、工、贸发展的道路,如山东大学生物系成立了昆虫蛋白研究所,华中农大植保系成立了昆虫资源研究室。他们相继建起了蝇蛆工厂,主副产品有蝇蛆蛋白粉、蛆油和脱乙酰甲壳素。就目前的技术,蝇蛆蛋白粉仅用于畜禽鱼配合饲料。经科学鉴定,人类可食昆虫达370…     

墨西哥食用昆虫的营养成分

墨西哥国立自治大学（UNAM）以胡列塔·拉姆斯·E．（JulietaRamosE．）女博士为首的食用昆虫研究课题组,自60年代以来,一直致力于食用昆虫的研究,特别是在营养成分分析方面,已引起世界注目。到目前为止,他们对100多种民间食用昆虫的3大营养要素,即能值、蛋白质、脂肪以及矿物质、粗纤维、游离氮素,蛋白质中的氨基酸组分等进行了测定和分析,结果表明,许多昆虫种类不仅蛋白质含量高于鸡、鱼、蛋和猪肉等高等动物的蛋白质含量,而且蛋白质中的氨基酸组分分布比例非常合理,接近于联合国粮农组织（FAO）制订的蛋白质中氨基酸比例…     

重组含有Epstein-Barr病毒潜伏膜蛋白1(LMP1)基因的杆状病毒在昆虫细胞中的表达

用杆状病毒表达系统重组病毒,在昆虫细胞中表达了完整的含有ＥＢＶ－ＬＭＰ１基因３个外显子开放读码框架的长２．３ｋｂ的ｃＤＮＡ片段。用重组病毒感染Ｓｆ９细胞,用免疫荧光染色,结果表明：４８小时表达重组蛋白,７２小时细胞较完整,免疫荧光染色强阳性,９６小时后细胞出现破碎。我们采集７２小时的组织培养上清和细胞破碎裂解液,分别采用ＳＤＳ－ＰＡＧＥ、ＨＰＬＣ分子筛法,用免疫蛋白印迹法实验证明,表达的蛋白能被抗ＬＭＰ１的单克隆抗体所识别,测定表达蛋白的分子量为６０ｋＤ。经蛋白含量扫描图分析,采用Ｓｅｐｈａｄｅｘ－７５柱初步纯化表达的ＬＭＰ１蛋白,将后者进行裸鼠体内致瘤实验,未见肿瘤生长。     

中国夜蛾科昆虫区系初步研究（鳞翅目：双孔亚目）

对我国分布记载较详细的１４１０种夜蛾科昆虫区系分析结果表明：１）在世界昆虫区系中,可分为１７个分布型,以东洋界种类最多,占３５．６％;古北界种类居第二,占３３．０％;古北界和东洋界共有种类居第三,占２３．０％。２）中国夜蛾科昆虫主要由古北界种类、东洋界种类、古北界和东洋界共有种类组成,占总数的９１．７％;与澳洲界共有６７种,占４．８％;与非洲界共有５１种,占３．６％;与新北界共有２３种,占１．６％