7型腺病毒E1A基因克隆及真核表达

为了研究7型腺病毒(Ad7)E1A在感染中的致病机制,分离了Ad7d2病毒株,构建了Ad7 E1A基因的真核表达体系.用A549细胞分离培养痰液标本中的腺病毒,应用重叠延伸PCR扩增Ad7 E1A基因外显子,产物克隆至真核表达载体pIRES-Neo,构建重组子pIRES-Neo-Ad7E1A并转染A549细胞,利用Western印迹对其表达产物进行鉴定.克隆测序显示扩增的Ad7 E1A基因外显子包含了完整的编码区基因,pIRES-Neo-Ad7E1A转染A549细胞的表达产物经Western印迹鉴定与设计相符.成功建立了Ad7 E1A 基因的真核表达体系.     

产S-酰胺酶培养基统计学筛选与响应面优化

利用Design Expert软件中的两水平实验设计和响应面法,对发酵生产S-酰胺酶(可用于拆分2,2-二甲基环丙甲酰胺外消旋体)的培养基进行了优化。采用Plackett-Burman(PB)设计对培养基中相关影响因素的效应进行评价并筛选出了有显著效应的葡萄糖、酵母粉及2,2-二甲基环丙甲酰胺浓度,其他因素对酰胺酶产量的影响不显著。然后用旋转中心组和实验设计及响应面分析确定了主要影响因素的最佳条件,在优化的培养基中,酰胺酶产量达到168 U/L,比优化前的80 U/L提高了110.0%。     

《自由组合规律试验》教学设计

一、教材依据 新课标中图版必修二《遗传与进化》第二单元第二章第一节自由组合规律试验。二、设计思想 普通高中生物课程标准中对该部分内容的要求是：阐明基因的自由组合规律。     

丙种免疫球蛋白Fc段糖基化及其生物学活性和功能

丙种免疫球蛋白是血清中重要的糖蛋白,在IgG Fc的297位天冬酰胺位点存在重要的糖基化,连接的糖链能维持IgG重链构像并调节Fc和FcγR结合能力,异常的糖链改变某些蛋白质的抗原特性,激活补体,介导炎症引起免疫失衡.多种疾病都可能伴有异常糖苷化或因缺少糖苷化酶引起.糖苷的功能结构研究已取得一定进展,进一步研究IgG的Fc段不同糖基化对IgG晶体结构及其生物学功能的影响将有助于设计新型生物制品.本文就近年来丙种免疫球蛋白Fc段糖基化及生物学活性与功能、糖蛋白寡糖链等研究进展作一综述.     

10种冬青属植物遗传多样性RAPD和AFLPs分析

采用RAPD和AFLP技术,对10种冬青属植物基因组进行DNA片段扩增,以研究该属种间遗传多样性.结果表明:在RAPD分析中,通过对100种10个碱基随机引物的筛选,发现11种引物能得到多态性较高扩增产物,11种引物共扩增出301条多态性条带,多态率为98.63%.在AFLP分析中,3对选择性引物组合均扩增出了丰富的多态性片段.利用RAPD和AFLP技术分析,结果按UPGMA类平均法进行聚类,聚类结果显示冬青和代茶冬青,木姜冬青和浙江冬青以及光枝刺缘冬青与毛枝三花冬青之间的亲缘关系最近.     

Microsatellite analysis of genetic diversity and population structure of Chinese mitten crab （Eriocheir sinensis）

Chinese mitten crab (Eriocheir sinensis) has higher commercial value as food source than any other species of Eriocheir in China.To evaluate the germplasm resources and characterize the genetic diversity and population structure of the crabs in different water systems,two stocks and two farming populations were assessed with 25 polymorphic microsallite loci available in public GenBank.Basic statistics showed that the average observed heterozygosity (Ho) amongst populations ranged from 0.5789 to 0.6824.However,a remarkable presence of inbreeding and heterozygote deficiencies were observed.To analyze population structure,pairwise FST coefficients explained only ～10.3% variability from the subdivision of mitten crab populations,the remaining variability stems from the subdivision within subpopulations.Although the four populations had slight differentiation,different allelic frequencies resulted in distinct population structures.Two stocks and one farming population were clustered together to the phylogenetic branch of Yangtze crab,with an approximate membership of 95%.Whereas,another fanning population was clustered singly to the phylogenetic branch of the Liaohe crab,with a membership of 97.1%.The tests for individual admixture showed that Yangtze crab had probably been contaminated with individuals from other water systems.Genetic relationships between populations also supported the conclusion that Yangtze crab and Liaohe crab had different gene pools in spite of the origins of the same species.     

The analysis of genetic diversity and differentiation of six Chinese cattle populations using microsatellite markers

A total of 321 individuals from six cattle populations of four species in a bovine subfamily in China were studied using 12 pairs of microsatellite markers. The genetic diversities within and between populations were calculated. The phylogenetic trees were constructed by （δμ）^2 and DA distances, and the divergence times between populations were estimated by （δμ）^2. Altogether, 144 microsatellite alleles were detected including 24 private alleles and nine shared alleles. Chinese Holstein had the largest number of private alleles （10）, whereas Bohai black and Buffalo had the smallest number of private alleles （2）. Chinese Holstein showed the highest genetic variability. Its observed number of alleles （Na）, mean effective number of alleles （MNA）, and mean heterozygosity （He） were 7.7500, 4.9722, and 0.7719, respectively, whereas, the Buffalo and Yak showed low genetic variability. In the phylogenetic trees, Luxi and Holstein grouped first, followed by Bohai and Minnan. Yak branched next and buffalo emerged as the most divergent population from other cattle populations. Luxi and Bohai were estimated to have diverged 0.039-0.105 million years ago （MYA）, however, buffalo and Holstein diverged 0.501-1.337 MYA. The divergence time of Yak versus Minnan, Holstein and buffalo was 0.136-0.363, 0.273-0.729, and 0.326-0.600 MYA, respectively.     

寻找非常规的替代化合物

在开发具有最长生命周期的受知识产权（IP）保护的新产品过程中,药物化学家们大都采用基于片段的设计策略。OSI药物制剂公司的计算化学家Kenneth Foreman说,这样的设计策略可以突破高通量筛选（HTS）的一些限制,并且可以“对文献中的信息起到杠杆放大作用”。     

“动物行为学高级培训班暨首次动物行为学研讨会”在京举行

“动物行为学高级培训班暨首次动物行为学研讨会”于2007年12月11~14日在中国科学院动物研究所成功举办,参会人员约150人。来自国内、外不同领域从事动物行为学研究的知名学者就精子竞争和近交回避(康乐)、行为的遗传和基因调节(孙中生)、行为的神经机制(罗敏敏、徐富强)、紫外光在蜘蛛通讯的作用(李代芹)、动物的声通讯和发声机制(沈钧贤、王丁、左明雪)、性比和共存(张大勇)、灵长类的社会行为(李进华)、痛觉心理(罗非)、鼠类的取食行为(张知彬和肖治术)、人与海豚的行为关系(Sara Platto)、动物行为与动物园设计(Carin Harrington)等应…