Physical mapping of three fruit ripening genes：Endopolygalacturonase,ACC oxidase and ACC synthase from apple（Malus x domestica） in an apple rootstock A106（Malus sieboldii

The apple rootstock,A106(Malus sieboldii),had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell.Karyotypes were prepared from root-tip cells with 2n=34 chromosomes,Seven out of 82 karyotypes(8.5%) showed one pari of satellites at the end of the short arm of chromosome 3.C-bands were shown on 6 pairs of chromosomes 2,4,6,8,14,and 16 near the telomeric regions of short arms.Probes for three ripening-related genes from Malus x domestica:endopolygalacturonase(EPG,0.6kb),ACC oxidase(1.2kb),and ACC synthase(2kb)were hybridized in situ to metaphase chromosomes of A106.Hybridization sites for the EPG gene were observed on the long arm of chromosome 14 in 15 out of 16 replicate spreads and proximal to the centromere of chromosomes 6 and 11.For the ACC oxidase gene,hylridization sites were observed in the telomeric region of the short arm of chromosomes 5 and 11 in 87% and 81% of 16 spreads respectively,proxiaml to the centromere of chromosome 1 in 81% of the spreads,and on the long arm of chromosome 13 in 50% of the spreads. Physical mapping of three fruit ripening genes in an apple rootstock A106.Twenty five spreads were studied for the ACC synthase gene and hybridization sites were observed in the telomeric region of the short arm of chromosome 12 in 96% of the spreads.chromosomes 9 and 10 in 76% of the spreads,and chromosome 17 in 56% of the spreads.     

多异瓢虫生物学的研究

多异瓢虫在山东省德州地区一年发生４代,部分个体５代,以成虫在杂草丛内、残枝落叶及土块下越冬。在自然变温下,卵、幼虫、预蛹、蛹、成虫产卵前期和全世代的发育始点和有效积温分别为１８．８±０．５２℃和１５．０日度、１６．９７±０．０３℃和７３．３日度、１５．９７±０．０１℃和１０．２３日度、１５．４７±０．０５℃和２３．０５日度、１７．６±０．０４℃和３４．１日度、１８．２±０．１０℃和１７０．３日度。据Ｈｏｌｉｎｇ圆盘方程测定,１─４龄幼虫和产卵前期成虫日最大捕食棉蚜量依次力６．７头、３６．４头、７７．５头、８１．３头和６８．０头。     

旋扭山绿豆根瘤菌MXDI6菌株氢酶诱导表达

在自生异养条件下,旋扭山绿豆根瘤菌ＭＸＤＩ６菌株的氢酶诱导表达受气相、ｐＨ值、镍等因子影响：氢酶表达的最适氧浓度为４％,最适氢浓度为１５％,二氧化碳没有明显影响;氢酶表达的ｐＨ值以５．０—６．０为宜;０．５μｍｏｌ／ＬＮｉＣｌ２明显促进吸氢活性,但镍浓度大于１μｍｏｌ／Ｌ则抑制吸氢活性．     

诺卡氏菌属GS-17耐热茁霉多糖酶的提纯和性质

诺卡氏菌属GS-17(Nocardia sp.GS-17)的耐热茁霉多糖酶(Pullulanase EC.3.2.1.41)的粗酶液经中空纤维柱超滤浓缩、羟基磷灰石柱层析和Pullulan-Sepharose 6B亲和层析,得到凝胶电泳均一的纯酶,比活提高264倍.酶作用最适温度为55℃,最适PH6.2,分子量140000,等电点pI为6.0.该酶水解茁霉多糖、支链淀粉和可溶性淀粉,但不水解糖原.酶在50℃作用于茁霉多糖的米氏常数K_m为0.90mg/ml,最大反应速度V_(max)为57μmol·min~(-1)·mg~(-1).Zn~(2 )、Fe~(3 )、Hg~(2 )、Cu~(2 )、Pb~(2 )和环状糊精对酶有抑制作用,Ca~(2 )对酶有激活作用.经蛋白质侧链化学修饰研究表明,色氨酸残基位于酶的活性位区.该酶是由1129个氨基酸残基组成的单肽链,酶的N末端序列经测定为:Ala-Gly-His-Gly-Pro-Asp-Val-Gln-Asp-Gly-     

山东昌乐早始新世五图组多瘤齿兽类(哺乳纲)

本文记述了一种产自山东五图盆地的多瘤齿兽,在牙齿形态上类似在古新世绝灭的Mesodma和Mimetodon,取名为道森拟间异兽（Mesodmopsdawsonae）。这是亚洲晚白垩世－早第三纪地层中首次发现新斜沟齿兽类（neoplagiaucid）多瘤齿兽。文中也简单地讨论了我国目前缺少直接与大部分五图哺乳动物有关的古新世哺乳动物的原因。     

异裂菊属两新种

异裂菊属两新种梁健英（广西植物研究所,桂林５４１００６）关键词菊科;异裂菊属;新种ＴＷＯＮＥＷＳＰＥＣＩＥＳＯＦＨＥＴＥＲＯＰＬＥＸＩＳＣＨＡＮＧ（ＣＯＭＰＯＳＩＴＡＥ）￥ＬｉａｎｇＪｉａｎｇｙｉｎｇ（ＧｕａｎｇｘｉＩｎｓｔｉｔｕｔｅｏｆＢｏｔａｎｙ...     

多效唑对香蕉试管苗生长的影响（简报）

多效唑对香蕉试管苗有明显的矮化和延缓生长作用。它与NAA配合使用能促进长根,加快成苗速度,而且苗壮、耐荫。与6－BA配合使用可有效地提高分化芽的增殖系数。分化芽粗壮鲜绿,成活率高。     

化学调节剂对水稻离体幼穗乙烯生成的影响（简报）

CoCl2、NiSO4及高浓度的GA3抑制水稻离体幼穗乙烯的生成,而2,4-D、Ag2S2O3和乙烯利则明显促进乙烯生成。玉米赤霉烯酮的抑制不明显。精胺和亚精胺的影响不大。     

多歧苏铁——中国苏铁属一新种

多歧苏铁 独把铁,独脚铁(云南屏边) 新种