峡山水库水利风景区

峡山水库坐落于山东半岛,是山东省第一大水库,在潍坊市潍河中游的昌邑、高密、诸城、安丘4县市交界处。库区气候宜人,风景优美,物产丰富,花草树木繁多,是集观光旅游、休闲垂钓于一体的大型天然场所。水库总库容14.05亿立方米,兴利库容5.03亿立方米。库区涉及范围大,包括县市4个、乡镇11个、移民村97个。水库始建于1958年11月,1960年9月建成蓄水,水库灌区设计灌溉面积153万亩,有效灌溉面积104万亩,是一座集防洪、灌溉、发电、水产养殖和城市以及工业供水等综合利用于一体的大型水利工程。     

“酶的研究与应用”专题的教学设计

依照课标精神对“酶的研究与应用”专题进行了较为详细的分析和设计，包括制定专题教学目标、提供教学建议等内容，并提供一个课题的教学设计。     

Structural features and molecular evolution of Bowman-Birk protease inhibitors and their potential application

The Bowman-Birk inhibitors (BBIs) are well-studied serine protease inhibitors that are abundant in dicotyledonous and monocotyledonous plants. BBIs from dicots usually have a molecular weight of 8k and are double-headed with two reactive sites, whereas those from monocots can be divided into two classes, one approximately 8 kDa in size with one reactive site (another reactive site was lost) and the other approximately 16 kDa in size with two reactive sites. The reactive site is located at unique exposed surfaces formed by a disulfide-linked β-sheet loop that is highly conserved, rigid and mostly composed of nine residues. The structural features and molecular evolution of inhibitors are described, focusing on the conserved disulfide bridges. The sunflower trypsin inhibitor-1 (SFTI-1), with 14 amino acid residues, is a recently discovered bicyclic inhibitor, and is the most small and potent naturally occurring Bowman-Birk inhibitor.Recently, BBIs have become a hot topic because of their potential applications. BBIs are now used for defense against pathogens and insects in transgenic plants, which has advantages over using toxic and polluting insecticides. BBIs could also be applied in the prevention of cancer, Dengue fever, and inflammatory and allergic disorders, because of their inhibitory activity with respect to the serine proteases that play apivotal role in the development and pathogenesis of these diseases. The canonical nine-residue loop of BBIs/STH-1 provides an ideal template for drug design of specific inhibitors to target their respective proteases.     

羊胎免疫调节因子制造工艺研究

目的:优选出羊胎免疫调节因子提取的最佳工艺。方法:采用正交实验设计L8(27),以淋巴细胞转化实验为考察指标进行实验,对提取过程中的6个指标进行了优化,确定了最佳提取工艺。结果:羊胎免疫调节因子最佳提取工艺为A1B2C2D1E2F2,即2月龄内羊胎粉碎10 m in,加1:4双蒸水常温下8000 r.m in-1离心后10K超滤膜超滤即得。     

粽管巢蛛和拟水狼蛛对稻纵卷叶螟和稻褐飞虱捕食作用研究

应用二次正交旋转组合设计方法建立粽管巢蛛和拟水狼蛛对稻纵卷叶螟和稻褐飞虱捕食作用的数学模型。结果表明,粽管巢蛛是稻纵卷叶螟的重要天敌,粽管巢蛛和拟水狼蛛均是稻褐飞虱的重要天敌。当稻褐飞虱密度较高时,随着蜘蛛密度的增加,由于蜘蛛之间的干扰作用,导致稻褐飞虱被捕食量下降。     

天目山下农家乐

1936年．天目山就设有一个专门的风景名胜管理处,接待着四方的游人,人称“小上海”。而今因为旅游的兴起．天目山更成了城市人的“后花园”。每年约有12万人来此观光、度假休闲。于是．催生了当地农民经营的客栈——农家乐。[编者按]     

上海：拯救母亲河

纵观中国城市发展史,可以发现上海的崛起其实并不早,甚至晚于处于同一地区的苏、杭,那它为什么能在短短百余年的时间,人一跃成为国际著名的大都市呢？所谓“因水而生,因水而兴”,上海的崛起与发展正好印证了这句话。     

解放军疾控所SPF动物房的工艺设计

解放军疾病预防控制所,为履行职能和任务,在业务办公大楼12层建设了SPF动物实验设施,面积约240 m^2。文章介绍了作者的设计思路和建设方案。同时还介绍了SPF动物房的建设经验。     

次氯酸钠离析法观测甘蓝叶片脉序的处理条件优化筛选

次氯酸钠（NaClO）离析法主要用于植物叶片表皮的观测,在研究过程中发现该法也可用于叶片脉序的观测。以甘蓝（Brassica oleracea var.capitata）为实验材料,采用二次正交设计方法对水煮时间、NaOH浓度、NaOH处理时间、NaClO浓度、NaClO处理温度和处理时间等各种处理条件进行优化筛选,以期得到适合于甘蓝叶片脉序观测的最佳处理条件组合。实验结果表明,新鲜甘蓝叶片水煮3分钟,10%的NaOH溶液60°C水浴处理2.5小时,3%的NaClO溶液40°C水浴离析2小时,叶片脉序的观测效果最佳。     

RAPD反应体系优化的研究

筛选随机扩增多态性DNA(RAPD)反应体系的最佳优化方案,以期获得稳定可靠的实验结果。选取对结果影响较大的4种因素(Taq酶、dNTP、引物、Mg2+)进行单因素设计,寻找最佳反应浓度。优化后得到4种因素的最佳反应浓度分别为Taq酶2.5U、dNTP 200μmol/L、引物1μmol/L、Mg2+2.5mmol/L。在优化的反应条件下,RAPD的稳定性和重复性均好。