山黧豆幼苗对干旱胁迫的生理响应

用PEG6000模拟干旱对山黧豆进行胁迫。结果发现,胁迫初期（0~48 h）,突然的水分亏缺使气孔导度（Sc）和蒸腾速率（Tr）迅速下降,而净光合速率（Pn）和水分有效利用率（WUE）基本维持稳定。胁迫后期（48~108 h）,上述四个光合指标均不同程度地向对照水平恢复;脯氨酸和相对电导率（REC）受胁迫强度影响较小,但与胁迫时间呈明显正相关,分别在48和60 h增幅最大,而且当REC上升至对照的50%左右时,脯氨酸含量已达对照的14倍之多。由此认为,山黧豆可能主要通过迅速减小Sc和Tr以及大量而急剧的积累脯氨酸来减轻干旱所造成的伤害。     

逻辑方法应用于遗传学概念教学

在遗传学概念的教学中运用逻辑方法,用概念定义方式帮助学生对概念内涵的理解,用概念划分的方法明确概念的外延.运用概念的正反例、变式等策略掌握概念本质特征.应用概念关系图构建遗传学概念之间的逻辑关系.     

变功率微波法制备壳聚糖絮凝剂的研究

研究了变功率下微波加热对甲壳素脱乙酰化反应和粘度的影响。结果表明:在4 80W下反应4min、然后在16 0W下反应6min的最佳功率组合下,可制得脱乙酰度77.3%、粘度4 6mPa·s的壳聚糖产品;与恒功率微波法相比,变功率微波法制备壳聚糖的脱乙酰度稍低,但粘度高出31% ,反应时间缩短三分之一,能耗降低6 0 %     

福建金线莲的化学成分研究Ⅲ

从福建金线莲(Anoectochilus roxburghii)乙醇提取物的己烷萃取部分分得3个化合物和-甾体混合物,三个化合物结构分别鉴定为Sorghumol(1),木栓酮(2)和棕榈酸(3),甾体混合物经EI-MS、ESI-MS／MS技术确定含有24-异丙烯基胆甾醇、开唇兰甾醇、β-谷甾醇、豆甾醇和菜油甾醇。所有化合物均为该植物首次报道,其中化合物1为首次从兰科植物中分得,并首次对其光谱数据进行了详细的分析,化合物2为从该属植物中首次分得。     

9F系列海洋真菌代谢产物分离纯化及稻瘟霉活性研究

自帕劳群岛的3株9F系列海洋真菌中分离得到4个化合物,采用MS,NMR等光谱技术,确定结构分别为trans-dehydrocurvularin（1）,terrein（2）,paxilline（3）和citreopyrone（4）,其对稻瘟霉P-2b菌株的最小抑制浓度分别为：10,100,1．56,3．12μg/mL。其中化合物1是首次从海洋真菌中分离得到。     

耐药性木霉菌株的筛选及其对灰霉病的防治

兽籁当莨8咖耋楹∞102025323840455055培养时间（h）Time（h）图1ELll2单细胞生长曲线Fig．13heC（Kllx3eofthecells’samountsofELll2510152530354050培养时间Time（h）图2不同发酵时间的酶活Fig．23hed~stolyticactivityoffem~ent’8processing异。许多研冗表明,微生物营乔和代掰荚型与共生长蚧境碉着很大的关系。所以在采样时选择肉类加为了提高生物菌剂对植物病害的防治效果和稳定性,达到生物农药和化学农药综合防治的目的,采用了紫外诱变处理与药物培养基驯化培养相结合的方法,分离筛选出对化学杀菌剂速克灵有显著抗性的突变型木霉菌株T-4。该菌株与亲本菌株相比其抑菌能力及耐受性均优于亲本木霉菌株。经菌和药的协同作用研究发现,木霉菌剂与速克灵配比为8：2条件下,协同抑菌率达84．3％;活体拮抗性测定防治效果达到81％。这表明综合利用生物与化学防治,不仅可以降低农药使用量,减轻对环境的污染。而且,对植物病害的防效均高于单独使用木霉菌剂和速克灵的防治效果。     

Isolation and Expression Pattern Analysis of Two Ferritin Genes in Tobacco

For understanding of the ferritin gene expression pattern and the mechanism of iron homeostasis in tobacco (Nicotiana tabaccum L.) plants, two full-length ferritin cDNAs, NtFerl and NtFer2, were isolated from tobacco seedlings and characterized. These cDNAs are 1 214 and 1 125 bp nucleotides and encode 251 and 259 amino acid residues, respectively. The deduced amino acid sequences showed that two tobacco ferritins share the same characteristics as the plant ferritins from Arabidopsis, soybean, and maize. Southern blotting analysis indicated that both NtFerl and NtFer2 were probably multicopy genes in the tobacco genome. Northern blotting analysis indicated that iron loading of tobacco plantlets increased the ferritin mRNA abundance and that NtFerl expression was higher and more sensitive to iron than NtFer2 expression. Furthermore, NtFerl was expressed in both leaves and roots, whereas NtFer2 was expressed mainly in leaves.     

Spatial heterogeneity and population risk analysis of cotton bollworm, Helicoverpa armigera, in China

In this study, semivariance was used to quantitatively measure the spatial heterogeneity for the egg population of cotton bollworm during a growing season. The typical characteristic parameters of theoretical semivariance models against lag distances were applied to measure components of spatial heterogeneity： trend, range, spatial dependence, and the strength of spatial dependence. Then, kriging interpolation was used to evaluate the population risk of cotton bollworm exceeding economic thresholds. From early June through early September, the population densities were sampled 10 times in the study field. Results showed that the spatial patterns were related to population density. For its low-density population, the spatially heterogeneous trends were usually of spherical shapes; but for highdensity ones, the trends shifted to Gaussian shapes. The spatial dependence appeared at varied distances ranging from 52 meters to 936 meters, and the spatial dependence was in the range of 0.39-288.60, which changed with population densities. While having high heterogeneity, the strength of spatial dependence became much stronger. Results of population risk analysis showed that there was a high risk during its early stages, especially in mid-June. In August, population risk was so low that it did not need to be controlled.     

不同地区根霉分离株的RAPD多样性分析

为了研究微生物的分子多样性,在获得系列米根霉分离物的基础上,应用经筛选的13条随机引物,对来自不同地区的18株米根霉分离物进行了RAPD分析,聚类结果表明,18株分离物在0.75的相异水平上可以聚为6个群体,其分子多样性同地理来源之间具有一定的相关性。     

60Coγ射线对高免卵黄液中霉形体杀灭作用的研究

用^60Coγ射线处理高免卵黄液,研究了不同剂量、剂量率对霉形体的灭活效果和对卵黄抗体的影响。结果表明,^60Coγ射线可以完全杀灭高免卵黄液中的霉形体,霉形体在卵黄液中的灭活剂量为6．0kGy;10．0kGy以下的辐照剂量不影响卵黄液中蛋白质含量、pH值和活性氧自由基,同时不影响抗体效价。