贵州地区临床分离近平滑念珠菌复合群分布及其生物膜形成相关基因研究

目的 研究贵州地区近平滑念珠菌复合群临床分布和药敏特点,检测近平滑念珠菌生物膜形成能力及其CDC28基因的表达水平。方法 收集贵州地区2所三级甲等医院送检标本中分离出的菌株,利用BACTEC^TM FX仪、Phoenix-100仪对菌种进行鉴定,通过基因测序对近平滑念珠菌复合群各分型菌株进行鉴定;采用ATB FUNGUS 3试剂盒检测菌株体外药物敏感情况;检测血源性近平滑念珠菌生物膜形成能力及利用qRT-PCR技术检测它们的CDC28基因表达水平。结果 共分离鉴定出163株近平滑念珠菌复合群,其中近平滑念珠菌Ⅰ型、Ⅱ型和Ⅲ型分别占73.0%、6.1%和20.9%;尿液和血液是分离菌株最多的标本(均为50株);血液途径感染的菌株中,Ⅰ型、Ⅱ型和Ⅲ型分别占54.0%、10.0%和36.0%;三种基因型菌株对常见抗真菌药物无明显耐药性;17株临床菌株中,16株(94.1%)具有生物膜形成能力,其中2株、4株和10株菌株生物膜形成能力强、一般、弱,CDC28在2株生物膜形成能力强以及3株生物膜形成能力一般的菌株中表达上调。结论 分离的近平滑念珠菌复合群以Ⅰ型感染为主,三种基...     

黄柄钙皮菌的生活史

利用基物培养、燕麦-琼脂培养技术及扫描电镜技术研究了黄柄钙皮菌的个体发育过程,在燕麦琼脂培养基上完成了从孢子到孢子的生活史。结果表明,生活史包括单核的黏变形体或游动胞、多核的营养体原质团以及孢子形成阶段。孢子球形,表面具疣突。孢子萌发为裂式,释放1黏变形体。黏变形体行变形运动,在有水的条件下,可转变为游动胞并游动。可观察到1长具极性的鞭毛。合子形成原质团。成熟原质团棕色。原质团类型为显型,具有扇形网络状菌脉。琼脂培养基上获得的黄柄钙皮菌孢子与野生型相似,并具有可育性。     

环磷酸腺苷参与哺乳动物卵泡发育的研究进展

环磷酸腺苷(cyclic adenosine monophosphate,cAMP)是哺乳动物体内重要且保守的第二信使之一,可通过转导细胞外信号参与调节多种器官和组织的发育及生理功能.已有研究显示,雌性哺乳动物卵母细胞减数分裂进程与cAMP的水平变化密切相关且受到严格调控.卵母细胞中cAMP主要由腺苷酸环化酶3(ade...     

东方蝾螈原肠形成期瓶状细胞的结构及其对胚胎发育的影响

以１００只东方蝾螈早期胚胎为材料,用ＳＥＭ和ＴＥＭ对瓶状细胞形态结构和除去瓶状细胞的胚胎进行观察,去除瓶状细胞后,胚胎的原肠形成不会中断,外包和内卷仍能正常进行,并可以发育成幼螈,但有部分胚胎原肠前端缺失,长期培养的蝾螈胚对针刺激有反应,幼虫阶段中自由活动,我们的结论是：１）东方蝾螈原肠胚的瓶状细胞除了原肠前端存在外,还有部分瓶状细胞与原肠长轴垂直,从力学角度推断瓶状细胞在原肠形成过程中不起主要作     

荷斯坦奶牛红毛性状的基因检测及红毛形成机制的探讨

荷斯坦奶牛黑素皮质素受体1(MC1R)基因310G缺失突变产生e等位基因,导致红白花毛色。实验利用PCR-RFLP和DNA测序技术建立了荷斯坦奶牛红毛性状的基因检测方法,并在4个荷斯坦奶牛全同胞家系中得到了证实,可见其可用于鉴定携带e等位基因的种公牛,以指导奶牛育种。通过分析黑素皮质素(MC)受体蛋白家族序列,找到了MC1R蛋白结构与功能的区域(TM3、TM6、TM7和EL3)。利用VHMPT软件预测MC1R突变蛋白的结构,结果显示其丢失了TM3、TM6、TM7和EL3区,也失去了与α-促黑素(α-MSH)结合的区域,最终导致红毛的产生。     

Uterine receptivity and the plasma membrane transformation

This review begins with a brief commentary on the diversity of placentation mechanisms, and then goes on to examine the extensive alterations which occur in the plasma membrane of uterine epithelial cells during early pregnancy across species. Ultrastructural, biochemical and more general morphological data reveal that strikingly common phenomena occur in this plasma membrane during early pregnancy despite the diversity of placental types--from epitheliochorial to hemochorial, which ultimately form in different species. To encapsulate the concept that common morphological and molecular alterations occur across species, that they are found basolaterally as well as apically, and that moreover they are an ongoing process during much of early pregnancy, not just an event at the time attachment, the term ‘plasma membrane transformation‘ is suggested which also emphasises that alterations in this plasma membrane during early pregnancy are key to uterine receptivity.     

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA.Four lines of the transgenic plants were self-crossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization.Two lines,D1232 and D1653,showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leafy head.Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage,and the cuttings from head leaves had more roots than rosette leaves at heading stage.It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin.These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant orgens and for the breeding of new variety with rapid growth trait.     

空间分布型的形成过程研究

具合影个研究了广义空间分布型和复合空间分布型的形成问题,解释了空间分布型的形成机理,并给出了１１种广义空间分布型和９种复事空间分布型;同时提出了空间分布型的３种判定方法：扩散系数法、空间相关法和参数变动法,它们以可用于区分常见的空间分布型,也可以用于判定分布型的形成过程;为研究空间分布型的形成机理和变化规模提供了有力的手段。以马尾松毛虫的空间分布型的研究结果表明,马尾松毛虫幼虫的空间分布型是复合负     

光照、湿度和培养基对苎麻疫霉卵孢子产生量的影响

采用3因素随机区组设计研究了光照、湿度和培养基对苎麻疫霉(hytophthoraboehmeriae)卵孢子产生量的影响。结果表明,各因子对卵孢子产生量的影响效应大小次序为培养基>光照>湿度,其中培养基和光照两因子影响均在0.01水平上显着。在供试的4种常用培养基上,卵孢子产生量的大小次序为：SLA培养基(SLA)>利马豆培养基(LBA)>V₆汁培养基(V₆A)>澄清的V₆汁培养基(V₆B).在设置的3种光照条件中,卵孢子产生量以连续黑暗处理最高,连续光照最低,光照与黑暗交替处理居中。3个试验因子间的所有互作对卵孢子产生量均有极显着的影响。在不同光照、湿度、培养基组合中,卵孢子产生量以低湿+连续黑暗+SLA组合最高,低湿+连续光照+V₆B组合最低。     

林隙（gap）更新动态研究进展

林隙（ｇａｐ）更新动态研究进展臧润国（中国林业科学研究院生态环境研究所,北京１０００９３）ＲｅｓｅａｒｃｈＡｄｖａｎｃｅｓｏｆＧａｐＲｅｇｅｎｅｒａｔｉｏｎＤｙｎａｍｉｃｓ．ＺａｎｇＲｕｎｇｕｏ（ＩｎｓｔｉｔｕｔｅｏｆＥｃｏｌｏｇｙａｎｄＥｎｖｉｒ...