生物产品的经济效益优化

为了优化经济效益，许多文献一般以现值作为目标函数，其贴现率的主观性很大．本文以内部收益率作为目标函数，从根本上排除了主观性，与实际目标较为相符．     

Anti-tumor activity and immunological modification of ribosome-inactivating protein （RIP） from Momordica charantia by covalent attachment of polyethylene glycol

Ribosome-inactivating proteins （RIPs） are a family of enzymes that depurinate rRNA and inhibit protein biosynthesis. Here we report the purification, apoptosisinducing activity, and polyethylene glycol （PEG） modification of RIP from the bitter melon seeds. The protein has a homogenous N-terminal sequence of N- Asp-Val-Ser-Phe-Arg. Moreover, the RIP displayed strong apoptosis-inducing activity and suppressed cancer cell growth. This might be attributed to the acti- vation of caspases-3. To make it available for in vivo application, the immunogenicity of RIP was reduced by chemical modification with 20 kDa （mPEG）2-Lys-NHS. The inhibition activity of both PEGylated and non- PEGylated RIP against cancer cells was much stronger than against normal cells, and the antigenicity of PEGylated RIP was reduced significantly. Our results suggested that the PEGylated RIP might be potentially developed as anti-cancer drug.     

大肠杆菌中核糖体蛋白质S12依赖链霉素突变抑制λN基因的表达

将质粒pKC101上λcI基因切除，建成新质粒pHD109。用F'cI857控制质粒pHD109上λN基因的表达。结果表明：（1）核糖体蛋白质S12依赖链霉素突变抑制λN基因的表达；（2）F’cI857对质粒pHD109上λN基因表达的调节，受温度的控制。     

非核糖体肽合成酶工程改造研究进展

非核糖体肽合成酶合成的非核糖体肽类天然产物具有丰富的结构和多样的功能,在医药、农业、工业等领域具有广泛的应用潜力.利用合成生物技术工程改造非核糖体肽合成酶,在微生物细胞工厂中组合生物合成新型非核糖体肽分子顺应绿色化学的发展理念,是国内外学者关注的热点.文中归纳了3种不同的非核糖体肽合成酶工程改造策略,并对近年来相关领域...     

利用CRISPR-Cas9系统与核糖体工程获得新型可利霉素产生菌

可利霉素(Carrimycin,CAM)是将异戊酰基转移酶基因(Isovaleryltransferase gene,ist)导入到螺旋链霉菌中产生的以异戊酰螺旋霉素(Isovalerylspiramycin,ISP)为主组分的抗生素.原工程菌中的ist基因与螺旋霉素(Spiramycin,SP)生物合成基因簇相距较远...