小鼠脑组织腺苷A2A受体缺失模型的建立与评价

制备了脑组织腺苷A2A受体基因缺失的小鼠模型并对该模型进行评价。在采用2次6.2 Gy X线间隔照射对敲除A2A受体基因的雌性C57BL/6小鼠进行清髓处理后, 将野生型雄性C57BL/6小鼠骨髓细胞移植到其体内, 使其脑组织的A2A受体仍保持缺失型。然后对移植效果进行鉴定, 并对模型的生理指标进行观察和评价。结果发现: 骨髓移植6周后, 受体小鼠的白细胞性染色体基因由雌性变为雄性; 骨髓中A2A受体阳性细胞率为94.85%, 而脑内A2A受体mRNA与A2A受体基因敲除小鼠比较, 无显著差异。模型小鼠除心率略低于野生型小鼠外, 在呼吸频率、脑含水量以及脑内谷氨酸含量等生理指标上均与野生型小鼠和A2A基因敲除小鼠无显著差异。     

铜绿假单胞菌Ⅲ型分泌系统的分子调控机制

铜绿假单胞菌是临床上重要的革兰氏阴性条件致病菌.通过Ⅲ型分泌系统,铜绿假单胞菌将其毒力因子注入到真核宿主细胞内部,逃避宿主巨噬细胞的吞噬降解,引起宿主相应的病理变化,是铜绿假单胞菌感染致病的重要原因.本文在简单介绍铜绿假单胞菌Ⅲ型分泌系统组成和功能的基础上,主要对调控T3SS基因转录表达的分子机制的研究进展进行综述和讨论.     

Ⅰ型蛋白磷酸酶研究进展

Ⅰ型蛋白磷酸酶(PP1)属丝/苏氨酸磷酸酶的一种,在生物体中广泛存在,参与调节多种重要的生理功能,包括转录、翻译、代谢、细胞生长及分化等.PP1分子结构表面的3个凹槽及β 12-β 13 Loop环结构,它在底物与抑制剂的结合方面起决定作用.近期研究发现,Loop环结构除了是抑制剂的结合部位之外,对整个酶分子的结构和性质都起重要作用.功能研究也证明PP1还参与HIV-1转录过程的调节,并且与老年性痴呆等多种疾病密切相关.主要对PP1的组织分布、分子结构、酶学特性、催化机制以及生物学功能等方面进行了相应的综述.     

植物细胞Ca2+的微调系统——Ca2+_ATPase

本文对植物体细胞Ca２＋-ATPase的类型、亚细胞定位、生化特性、分子量差异、基因克隆、酶活性调节剂以及生理功能等方面的研究进展进行综述和讨论。     

表达EB病毒主要膜蛋白gp350／220的非复制型重组痘苗病毒的构建

利用非复制痘苗病毒质粒载体pNEOCK11β75及pNEOCK,改造了表达EB病毒主要膜蛋白gp350/22的复制型重组痘苗病毒VMA,构建了非复制型重组痘苗病毒VMA△CK。该病毒能在鸡胚原代成纤维细胞（CEF）中正常繁殖,而在人源细胞中不能正常繁殖。在CEF中连续传代至第25代,经PCR证明,该病毒符合非复制型重组痘苗病毒的特征。经免疫荧光及免疫酶斑法证实,VMA△CK可稳定表达gp350/220,且表达水平与VAM无明显差异。VMA△CK经腹腔免疫Balb/C小鼠,4周后能诱生一定水平的抗gp350/220特异性抗体,加强免疫2周后该抗体水平明显升高。这一结果类似于VMA免疫Balb/C小鼠的结果,初免后,VMA△CK且抗痘苗抗体水平明显低于VMA免疫组;加强免疫2周后,两组小鼠的抗痘苗抗体水平趋于一致。上述结果证明,所构建的非复制痘苗病毒不影响目的抗原的表达,也不影响该抗原的免疫原性,但导致病毒毒力下降,而且用该病毒免疫小鼠后小鼠抗痘苗病毒载体的免疫反应明显下降。     

Blockage of IGF－1R signaling sensitizes urinary bladder cancer cells to mitomycin－mediated cytotoxicity

INTRODUCTIONtransitional cell carcinoma (TCC) of the bladder represents the fifth most preValent malignancy inwestern population. A major problem in the management of TCC is the low sensitivity to chemotherapy and the high recu-rrence after transurethral resection, which occupies a large proportion (approximately 40%) among bladder cancer patients[1, 21. Sodrug resistance remains a major and difficult problem to resolye in TCC chemotherapy. This phenomenon has often been ascribed to so…     

合成肽抗原在戊型肝炎病毒感染诊断中的应用

An ELISA for the detection of anti HEV using synthetic peptide antigens was developed. The synthetic antigens were encoded by OFR2 and OFR3 genes of HEV. The purpose of this study was to determine the applicability of the synthetic antigens in the serodiagnosis of hepatitis E. The anti HEV detection using synthetic antigens was carried out in 47 healthy subjects and 89 patients with acute or chronic viral hepatitis. The results showed that the positive rate of anti HEV IgG in healthy subjects was 4.2%(2/47), and no IgM antibody to HEV was found. The positive rates of IgG and IgM antibodies to HEV in the hepatitis patients were 8.9% and 10% respectively. In addition, we compared the detecting efficacy of the synthetic antigens with that of the market reagent in 57 serum samples, the total coincident rate was 87.7% (50/57). All of the results accorded with the literatures reported. This study suggests that the ELISA based on the synthetic peptide antigens was specific, sensitive and convenient in diagnosis of HEV infection, it can be widely used in both clinical and epidemiological reseaches.