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171.
William Parker Todd A. Wells Susanne Meza-Keuthen In-Soo Kim Pill-Soon Song 《Journal of Protein Chemistry》1995,14(2):53-57
Recently, Mummertet al. [Nature
363, 644–648 (1993)] isolated a proposed TCP1-related chaperone. Here we report several findings concerning the protein which they sequenced. Two similar N-terminal sequences were obtained from this abundant 60-kDa protein. Internal sequences were also acquired by protease digestion. Initially it was believed the protein was able to completely inhibit citrate synthase aggregation, but later purifications demonstrated that the 60-kDa polypeptide lacked both chaperone activity and the previously reported kinase activity [Grimmet al., Planta
178, 199–206 (1989)]. It is now our belief that this protein is neither a chaperone nor a kinase. 相似文献
172.
Six polyacetylenes have been isolated from immature seeds of safflower (Carthamus tinctorius L.) by thin-layer chromatography. They were identified as 1,11-tridecadiene-3,5,7,9-tetrayne, 1,3,11-tridecatriene-5,7,9-triyne, 1,3,5,11-tridecatetraene-7,9-diyne, 1-tridecene-3,5,7,9,11-pentayne, 1,3-tridecadiene-5,7,9,11-tetrayne and 1,3,5-tridecatriene-7,9,11-triyne from the results of their spectroscopic and chemical analyses. Three of these polyacetylenes had not been isolated from Carthamus tinctorius L.Changes in the polyacetylene content during maturation were followed by the measurement of ultraviolet absorbance. While 1,3,11-tridecatriene-5,7,9-triyne and 1,3,5,11-tridecatetraene-7,9-diyne had already occurred abundantly at the day of flowering, the amounts of the other polyacetylenes reached the maximum values at the fourth to sixth days after flowering. Although the total amounts of the six polyacetylenes were about 0.8 mmole/g lipid at the fourth day after flowering, no polyacetylene was detected in the mature seeds. 相似文献