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71.
P. Witzgall W. Sauter H.-R. Buser S. Rauscher H. Arn P. J. Charmillot Th. Wildbolz 《Entomologia Experimentalis et Applicata》1989,53(2):133-136
As shown by chemical analysis and field trapping, the sex pheromone of Grapholita lobarzewskii consists of a blend of (E)-8-dodecenyl acetate and (Z)-8-dodecenyl acetate. Maximum attractiveness was found at 80–95%. Dodecyl acetate and tetradecyl acetate, both present in the female gland, did not affect trap catch. G. lobarzewskii has recently gained importance as a pest of apple and plum, but has so far been referred to as Grapholita janthinana. The latter is attracted to the same two compounds, but in reversed portions (20% E).
Zusammenfassung Chemische Analysen und Feldversuche zeigen, dass das Sexualpheromon des Kleinen Fruchtwicklers, G. lobarzewskii aus (E)-8-Dodecenylacetat (Z8-12:Ac) und (Z)-8-Dodecenylacetat (Z8-12:Ac) besteht. Die grösste Lockwirkung wurde mit einem Gemisch der beiden Substanzen bei einem E-Anteil von 80–95% erzielt. Dodecylacetat (12:Ac) und Tetradecylacetat (14:Ac) wurden ebenfalls in den weiblichen Pheromondrüsen nachgewiesen, hatten aber im Freiland keinen Einfluss auf die Lockwirkung. G. lobarzewskii tritt seit einiger Zeit in der Schweiz als Schädling von Apfel und Zwetschge auf und ist an ihrem charakteristischem Frassgang leicht erkennbar. Die Art wurde bisher irrtümlich als G. janthinana bezeichnet. G. janthinana lebt auf Rosaceen, und wird vom gleichen Substanzpaar angelockt, allerdings bei umgekehrtem Mischungsverhältnis (20% E).相似文献
72.
73.
20-羟蜕皮素对家蚕后部丝腺细胞超微结构的影响 总被引:7,自引:1,他引:6
应用超薄切片和电镜技术,详细观察了蜕皮激素(MH)对家蚕Bombyx mori后部丝腺细胞超微结构的影响.电镜观察表明,家蚕后部丝腺细胞对MH处理极为敏感.一经MH处理,其细胞核的形态结构和细胞质中各种细胞器的发生、发展都呈现出明显的变化,并且与MH处理时期及剂量有关:五龄前期适量MH(4μg/头)处理,能促进与丝蛋白合成有关细胞器的形成与发展,加速腺细胞的成熟生长;较高剂量(8—16μg/头)处理,则导致自噬体的过早发生.五龄中、后期MH处理,一方面促进了粗面内质网等细胞器的进一步发达,另一方面也提高了自噬体的发生数量;处理剂量越高,后一种倾向越突出.这些结果证明,后部丝腺细胞超微结构的变化受MH调节. 相似文献
74.
The adenylyl cyclase system of the yeast Saccharomyces cerevisiae contains the CYR1 polypeptide, responsible for catalyzing formation of cAMP from ATP, and two RAS polypeptides, responsible for stimulation of cAMP synthesis by guanine nucleotides. We have obtained rabbit antibodies that recognize the CYR1 protein. Antibodies were raised against synthetic oligopeptides and against a recombinant beta-galactosidase/CYR1 fusion protein. These antibodies have allowed the identification of the CYR1 gene product as a 205 kDa protein. Treatment with trypsin (2 micrograms/ml) reduced the size of the CYR1 protein from 205 to 155 kDa and produced an activated enzyme which no longer responded to guanine nucleotides. This result is consistent with a model in which adenylyl cyclase activity is regulated by an inhibitory domain near the amino-terminus of the CYR1 protein. This model is further supported by the finding that adenylyl cyclase activity is also markedly elevated and unresponsive to guanine nucleotides in mutant yeast strains that express only the carboxy-terminal half of the CYR1 protein. Treatment with high trypsin concentrations (greater than 10 micrograms/ml) caused release of adenylyl cyclase activity from the membrane. Comparison of immunoreactive CYR1 fragments released by trypsin and membrane bound genetically altered proteins suggests that the CYR1 protein is attached to the membrane via a separate trypsin sensitive anchoring protein rather than via a membrane anchoring domain. 相似文献
75.
We have studied the mechanisms of breakdown of 2'-5' oligoadenylates. We monitored the time-courses of degradation of ppp(A2'p5')nA (dimer to tetramer) and of 5'OH-(A2'p5')nA (dimer to pentamer) in unfractionated L1210 cell extract. The 5' triphosphorylated 2'-5' oligoadenylates are converted by a phosphatase activity. However, 2'-5' oligoadenylates are degraded mainly by phosphodiesterase activity which splits the 2'-5' phosphodiester bond sequentially at the 2' end to yield 5' AMP and one-unit-shorter oligomers. The nonlinear least-squares curve-fitting program CONSAM was used to fit these kinetics and to determine the degradation rate constant of each oligomer. Trimers and tetramers, whether 5' triphosphorylated or not, are degraded at the same rate, whereas 5' triphosphorylated dimer is rapidly hydrolyzed and 5'-OH dimer is the most stable oligomer. The interaction between degradation enzymes and the substrate strongly depends on the presence of a 5' phosphate group in the vicinity of the phosphodiester bond to be hydrolyzed; indeed, when this 5' phosphate group is present, as in pp/pA2'p5'A/or A2'/p5'A2'p5'A/, affinity is high and maximal velocity is low. Such a degradation pattern can control the concentration of 2'-5' oligoadenylates active on RNAse L either by limiting their synthesis (5' triphosphorylated dimer is the primer necessary for the formation of longer oligomers) and/or by converting them into inhibitory (e.g., monophosphorylated trimer) or inactive (e.g., nonphosphorylated oligomers) molecules. 相似文献
76.
The cytochrome b-f complex is composed of four polypeptide subunits, three of which, cytochrome f, cytochrome b-563 and subunit IV, are encoded in chloroplast DNA and synthesised within the chloroplast, and the fourth, the Rieske FeS protein, is encoded in nuclear DNA and synthesised in the cytoplasm. The assembly of the cytochrome b-f complex therefore requires the interaction of subunits encoded by different genomes. A key role for the nuclear-encoded Rieske FeS protein in the assembly of the complex is suggested by a study of cytochrome b-f complex mutants. The assembly of individual subunits of the complex may be regulated by the availability of prosthetic groups. The genes for the chloroplast-encoded subunits and cDNA clones for the Rieske FeS protein have been isolated and characterised. Cytochrome f and the Rieske FeS protein are synthesised initially with N-terminal presequences required for their correct assembly within the chloroplast. The deduced amino acid sequences of the four subunits have been used to suggest models for the arrangement of the polypeptides in the thylakoid membrane. 相似文献
77.
78.
G Floyd-Smith 《Journal of cellular biochemistry》1988,38(1):13-21
The levels of a (2'-5')An-dependent endonuclease (RNase L) were determined in extracts prepared from murine L cells and Ehrlich ascites tumor (EAT) cells by measuring specific binding of protein to a labeled derivative of (2'-5')An, (2'-5')A3[32P]pCp. RNase L levels were found to depend both on interferon (IFN) treatment and on cell growth conditions. Treatment of murine L cells and EAT cells with 100-2,000 IRU IFN beta or IFN gamma resulted in a similar 2-4-fold increase in the levels of RNase L when cells were present at low density. The levels of RNase L were also shown to increase 2-3-fold as cells approached saturation density. Serum-starved cells also displayed relatively high levels of RNase L. RNase L levels in cells maintained at high cell density did not change appreciably following treatment with IFN beta or IFN gamma. Regulation of RNase L levels by cell growth conditions as well as by IFN beta or IFN gamma treatment suggests that RNase L may play an important role in regulating the levels of cellular mRNAs as well as acting to degrade viral RNAs. 相似文献
79.
The possibility of interaction of hepatocytes with the heparin binding domain of Fibronectin was examined. Rat hepatocytes
adhered to coverslips coated with the 33-kDa heparin binding fragment of the C-terminal region of plasma fibronectin. When
different concentrations of the heparin binding fragment were used to coat coverslips and used as substratum, cell attachment
showed saturation kinetics. Half the maximum attachment was observed at 30–40 min after seeding of cells. The cells became
flat after 2–3 h indicating that they spread on the heparin binding domain as they do on intact fibronectin. Among the different
glycosaminoglycans tested, maximum inhibition of attachment was observed for heparin. However it was not possible to completely
inhibit attachment even at high concentrations. These results indicate that hepatocytes interact with fibronectin not only
through the Arg-Gly-Asp-containing cell binding fragment, but also through the heparin binding domain of fibronectin and,
further, that there exist heparin-dependent and heparin-independent mechanisms of interaction of cells with the 33-kDa heparin
binding fragment of fibronectin 相似文献
80.
Roman G. Efremov Dmitry I. Gulyaev Gerard Vergoten Nikolai N. Modyanov 《Journal of Protein Chemistry》1992,11(6):665-675
A new computer-aided molecular modeling approach based on the concept of three-dimensional (3D) molecular hydrophobicity potential has been developed to calculate the spatial organization of intramembrane domains in proteins. The method has been tested by calculating the arrangement of membrane-spanning segments in the photoreaction center ofRhodopseudomonas viridis and comparing the results obtained with those derived from the X-ray data. We have applied this computational procedure to the analysis of interhelical packing in membrane moiety of Na+, K+-ATPase. The work consists of three parts. In Part I, 3D distributions of electrostatic and molecular hydrophobicity potentials on the surfaces of transmembrane helical peptides were computed and visualized. The hydrophobic and electrostatic properties of helices are discussed from the point of view of their possible arrangement within the protein molecule. Interlocation of helical segments connected with short extramembrane loops found by means of optimization of their hydrophobic/hydrophilic contacts is considered in Part II. The most probable 3D model of packing of helical peptides in the membrane domain of Na+, K+-ATPase is discussed in the final part of the work. 相似文献