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991.
The effects of hyper- and hypo-saline stresses on the levels of various inorganic and organic solutes inUlva lactuca have been recorded. Hypoosmotic stress decreased the tissue concentration of K+, Na+ and Cl- while hyper-osmotic stress caused a transient increase in Na+ and a stable accumulation of K+ and Cl-. The tissue content of -dimethylsulphoniopropionate (-dimethylpropiothetin) responded to changes in salinity. The time course of hypersaline stress showed the -dimethylsulophoniopropionate concentration rose as the Na+ level fell. The levels of free sugars and amino acids, including proline, were relatively low in this alga and did not appear to be important in osmotic adjustment. The possibility that tertiary sulphonium dipolar ions have an analogous role in some algae to glycinebetaine and possibly other quaternary nitrogen compounds in higher plants as cytoplasmic osmotica is discussed briefly.Abbreviations DMSP
-dimenthylsulphomiopropionate
- AFT
apparent free space
- TLE
thin layer electrophoresis
- NPS
ninhydrin positive substances
- TLC
thin layer chromatography 相似文献
992.
Activities of Phosphorylase, glyceraldehyde-3 -phosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase and succinate
dehydrogenase in the rat endometrial tissue are significantly inhibited by an intrauterine copper device, while it stimulated
glucose-6-phosphate dehydrogenase activity. The copper device decreased the lactate/pyruvate ratio in the tissue; pyruvate
utilizationin vitro by the rat endometrium is also blocked by copper. These findings suggested that the normal carbohydrate metabolism of the
tissue may be affected in presence of copper, thus resulting in a change of the endometrial function, which may be one of
the factors responsible for the contraceptive and pharmacological action of an intrauterine copper device. 相似文献
993.
K. Watanabe S. Hakomori M.E. Powell M. Yokota 《Biochemical and biophysical research communications》1980,92(2):638-646
Two amphipathic protein fractions soluble in organic solvents as well as in water have been isolated from the ganglioside fraction of bovine erythrocyte membranes by successive chromatography in chloroform-methanol mixture on DEAE-Sephadex, silicic acid, and α-hydroxypropylated Sephadex G50 (LH60) columns. These two fractions contained a similar low molecular weight protein but with distinctively different amino acid composition. One of these proteins has been characterized by having a strong Paul-Bunnell antigen activity and had a binding affinity to ganglioside. A similar protein without Paul-Bunnell antigen activity was isolated as the major ganglioside-associated protein. 相似文献
994.
During chloroplast development in the primary leaves of Phaseolus vulgaris, the extractable activity of adenosine 5-phosphosulfate sulfotransferase increased ten-fold. When chloroplast development took place in air enriched with 3.5 l H2S·l-1 there was a decrease in adenosine 5-phosphosulfate sulfotransferase activity. Cyst(e)ine in concentrations up to 1 mM (in the external medium) did not affect the increase in adenosine 5-phosphosulfate sulfotransferase activity in intact plants. In plants with excised roots, 0.75 mM cyst(e)ine inhibited this increase. In green primary leaves, H2S or cyst(e)ine treatment resulted in a decrease of extractable adenosine 5-phosphosulfate sulfotransferase activity. In intact plants, this effect of cyst(e)ine was observed at a concentration of 1 mM, and in plants with excised roots, 0.25 mM had a comparable effect.In developing plants, the extractable activities of O-acetyl-L-serine sulfhydrylase (EC 4.2.99.9) and ribulosebisphosphate carboxylase (EC 4.1.1.39.) were not affected by H2S or cyst(e)ine.Abbreviations APS
adenosine 5-phosphosulfate
- APSSTase
adenosine 5phosphosulfate sulfotransferase
- BSA
bovine serum albumin
- DTE
dithioerythritol
- EDTA
ethylenediaminetetra-acetic acid
- OASSase
O-acetyl-L-serine sulfhydrylase
- PAPS
adenosine 3-phosphate 5-phosphosulfate
- POPOP
1,4 Di 2-(5-phenyloxazolyl)-benzene
- PPO
2,5-diphenyloxazol
- RubP
ribulose-bisphosphate
- RubPCase
ribulosebiphosphate carboxylase
This is no. 8 in the series Regulation of Sulfate Assimilation in Plants. The term cysteine is used when it is clear that cystine is not involved; cyst(e)ine is used for an undefined mixture of cysteine and cystine. The concentrations are expressed in all cases relative to cysteine 相似文献
995.
Both enantiomers of -aminooxy--phenylpropionic acid (AOPP), potent inhibitors of L-phenylalanine ammonia-lyase, and their N-benzyloxycarbonyl (N-BOC) derivatives inhibit anthocyanin formation in developing flowers of Ipomoea tricolor Cav. and Catharanthus roseus Don. as well as in seedlings of Brassica oleracea var. caulo-rapa DC (kohlrabi) and B. oleracea var. capitata L. (red cabbage) with little interference with their normal development. Kohlrabi seedlings tolerate up to 0.3 mM L-AOPP and N-BOC-L-AOPP without a reduction of fresh weight or chlorophyll content, while anthocyanin is reduced to less than 20%.Abbreviations AOPP
-aminooxy--phenylpropionic acid
- N-BOC
N-benzyloxycarbonyl
- PAL
L-phenylalanine ammonia-lyase (EC 4.3.1.5) 相似文献
996.
The initiation of sporangiophores of Phycomyces was analyzed under oxygen-limiting conditions. Mutants lacking -carotene have a higher oxygen threshold than the wild type depending on the residual amount of -carotene. The supersensitivity to low oxygen tension is specific for sporangiophore initiation and can be suppressed by addition of either retinal, retinol or retinol acetate to the medium. It is suggested that retinol is a natural regulator of differentiation in Phycomyces. 相似文献
997.
Y J Abul-Hajj 《Steroids》1979,33(1):115-124
Steroid delta 4-5 alpha- and delta 4-5 beta-reductase activity was determined in 16 human mammary tumors and 8 DMBA-induced rat mammary tumors using a spectrophotometric assay. Steroid delta 4-5 alpha-reductase was present in all tumors investigated while delta 4-5 beta-reductase was detected in only 6 estrogen receptor negative human breast tumors and absent in all estrogen receptor positive human breast tumors as well as in all rat mammary tumors. Further support for the presence of delta 4-5 beta-reductase was established by using a dual-labelling technique consisting of incubating tumor slices with [14C] testosterone and adding [3H] etiocholanolone, [3H] testosterone and [3H]-5 alpha-dihydrotestosterone at the end of the reaction. Following extraction and chromic acid oxidation, 4-androstenedione, 5 beta-androstanedione and 5 alpha-androstanedione were isolated and purified, and the constancy of the 14C/3H ratio was used as proof of 5 alpha-reductase and 5 beta-reductase. These results were shown to be consistent with the data obtained using the spectrophotometric assay. 相似文献
998.
Alwyn H. Gentry 《Plant Systematics and Evolution》1981,137(1-2):95-105
Field studies in Amazonian Peru show that thePassiflora vitifolia complex, a microcosm of many larger taxa, is concentrated in Amazonia with 4 ecologically separated species. One species is restricted to seasonally inundated forests, a second to non-inundated white sand soils, a third to non-inundated lateritic soils, and the fourth mostly to non-inundated alluvial soils. A new combination is formally proposed to recognize the white sand taxon, previously regarded as a variety, as a distinct species:P. involucrata. The importance of -diversity in maintaining high Amazonian species diversity is emphasized. 相似文献
999.
1000.
Central neural peptides and catecholamines in spontaneous and DOCA/salt hypertension 总被引:1,自引:0,他引:1
Hypothalamic and neurophypophyseal levels of catecholamines and peptides were measured in spontaneous and deoxycorticosterone (DOCA)/salt hypertension. Catecholamines, norepinephrine, epinephrine and dopamine were measured by electrochemical detection while the peptides, vasopressin, oxytocin, luteinizing hormone-releasing hormone (LHRH), the enkephalins and somatostatin (SRIF) were measured by radioimmunoassay. Blood pressure was significantly elevated in both groups as compared to their controls. Marked changes in central neural peptides were observed in the SHR, while no differences were seen in DOCA/salt hypertension. Hypothalamic vasopressin, oxytocin, LHRH and SRIF were significantly decreased. In the posterior pituitary, enkephalins were increased twofold in the SHR. With regard to catecholamines, there was no change in hypothalamic content. However, a dramatic decrease in neurohypophyseal dopamine was observed in SHR. Plasma levels of vasopressin were significantly elevated in both types of hypertension while oxytocin was increased only in the DOCA/salt model. These result show that (1) a wide spectrum of neuroendocrine changes are associated with genetic hypertension, (2) there are CNS differences between DOCA/salt and spontaneous hypertension, and (3) central aminergic changes may be involved in th neuroendocrine alterations seen in the SHR. 相似文献