An alternate carbon source for enhancing production of polysaccharides by Silene vulgaris callus

Pectin termed silenan and acidic arabinogalactan were isolated as cell-wall polysaccharides of Silene vulgaris callus in the presence of various carbon sources as components of the media. The maximum yields, productivity per litre of medium and production per day of acidic arabinogalactan, were achieved using glucose or galactose as the carbon source. Sucrose was found to increase the production of the polysaccharides. Yields, productivity and rate of production of arabinogalactan per day were decreased in the presence of arabinose. Yields of silenan, productivity and rate of production per day were closely related irrespective of the sugar used as the carbon source in the media (sucrose, glucose or galactose) and yields of silenan from the callus growing on arabinose were comparable. A concentration of sucrose in the 20-50 g/L range enhanced the biosynthesis of silenan and at 50 g/L the silenan contained the linear backbone and the ramified regions of the macromolecule.     

Enhancing Biological Control of Leafrollers (Lepidoptera: Tortricidae) by Sowing Buckwheat (Fagopyrum esculentum) in an Orchard

Two successive trials were carried out in stone-fruit and apple orchards in the summers of 1994-95 and 1995-96 respectively to determine the effect of floral resources on leafroller parasitoid numbers. Orchards were divided into eight blocks, each containing one replicate of a control and a treatment. The treatment had buckwheat (Fagopyrum esculentum Moench) drilled (as 3 m 2 m plots) in the centre of each replicate, while the control areas were kept weed free with herbicides. Catches of parasitoids in yellow pan traps were compared between the control and treatment areas. In the first trial, on average 10 times more parasitoids were captured in the buckwheat plots than in the controls. However, few of the parasitoids were natural enemies of leafrollers; the catches were dominated by Anacharis sp., a parasitoid of the brown lacewing ( Micromus tasmaniae Walker), itself a beneficial insect. Leafrollers were very rare in this orchard, which explains the absence of their parasitoids in the traps. In the second season, with the experiment conducted in an orchard with a history of leafroller populations, higher numbers of Anacharis sp. were again caught in buckwheat than in control plots. However, significantly higher (34% compared with 20%) levels (P< 0.05) of parasitism (by Dolichogenidea tasmanica (Cameron)) of released leafroller larvae were observed in buckwheat plots than in controls, despite the small plot size. Also, higher numbers of D. tasmanica were captured in yellow sticky traps 2 m above the buckwheat than in the equivalent position in the control plots.     

The effect of sampling height on dendroclimatic analysis

The radial growth response to climate of white spruce in open grown conditions was examined at two measurement heights [breast height (1.3 m aboveground) versus stump height (0.3 m aboveground)] in order to examine the role of sampling height in dendroclimatic analysis. Twenty-four white spruce [Picea glauca (Moench) Voss] islands were sampled within Epinette prairie in the Spruce Woods Provincial Park (SWPP) located in south-western Manitoba. The structure of breast height and stump height residual chronologies were essentially the same after standardization and autoregressive (AR) modeling to stabilize growth variability, and AR modeling to remove temporal autocorrelation and thereby reduce low-frequency variation and enhance high-frequency variation. Correlation and response function analysis indicated that white spruce at the two sampling heights responded similarly to total precipitation, moisture index (precipitation subtract potential evapotranspiration), and minimum, mean, and maximum temperature. Both sampling heights also responded strongly to the cumulative effects of precipitation during the period of August of the previous year (t−1) to July of the current year (t). Regardless of the sampling heights considered, the results indicate that white spruce at its southern distribution limit responded strongly to moisture stress intensified by high temperatures. The results support the recommendation, at least for samples of white spruce at its southern distribution limit, that samples taken at stump height can be safely incorporated into tree-ring chronologies without the risk of diminishing the strength of chronologies in detecting climatic signals.