Influence of exercise on cardiac and skeletal muscle myofibrillar proteins

The purpose of this study was to examine the Ca²⁺-Mg²⁺ myofibrillar ATPase and protein composition of cardiac and skeletal muscle following strenuous activity to voluntary exhaustion. Sprague-Dawley rats (200 g) were assigned to a control and exercised group, with the run group completing 25 m·min^–1 and 8% grade for 1 hour. Following activity, the myocardial Ca²⁺–Mg²⁺ myofibrillar ATPase activity -pCa relationship had undergone a rightward shift in the curve. Electrophoretic analysis revealed a change in the pattern of cardiac myofibrillar protein bands, particularly in the 38–42 Kdalton region. Enzymatic analysis of myofibrillar proteins from plantaris muscle, revealed no change in Ca²⁺ regulation following exercise. Electronmicrographic and electrophoretic analysis revealed extensively disrupted sarcomeric structure and a change in the ratio of several plantaris myofibrillar proteins. No difference was observed for myosin: Actin: tropomyosin ratios; however a dramatic reduction in 58 and 95 Kdalton proteins were evident. The results indicate that prolonged running is associated with similar responses in cardiac and skeletal muscle myofibrillar protein compositions. The abnormalities in myofibrillar ultrastructure may implicate force transmission failure as a factor in exercised-induced muscle damage and/or fatigue.     

静态负荷诱发肌肉疲劳后恢复期sEMG信号变化规律

目的:探讨肌肉疲劳过程中sEMG功率谱变化与H 的关系以及可能存在的其它影响因素.方法:利用肌肉进行疲劳收缩结束后,短时间内肌肉pH值尚无明显改变的特性,观察恢复期30 s内s EMG功率谱的变化规律.八名男性受试者,以肱二头肌为目标肌肉,负荷强度为60%MVC,静态持续负荷至疲劳点后,在恢复期以同样负荷分别观察2 s、4 s、6 s、8 s、10 s、20 s、30 s时的sEMG信号特征.结果:肱二头肌在以60%MVC静态疲劳负荷过程中MPF呈线性下降.在疲劳负荷后的恢复期,MPF恢复极其迅速,运动结束后仅2 s,MPF已恢复到整个下降范围的26.5%;至30 s,MPF已恢复到整个下降范围的87.7%.结论:由[H ]增加引起的肌纤维动作电位传导速度下降不是决定sEMG功率谱左移的唯一因素,提示sEMG功率谱左移可能与神经源性的中枢机制的作用有关.     

Progressive resistance training in neuromuscular patients. Effects on force and surface EMG

In a randomized clinical trial the efficacy of strength training was studied in patients with myotonic dystrophy (n=33) and in patients with Charcot-Marie-Tooth disease (n=29). Measurements were performed at the start and after 8, 16 and 24 weeks of progressive resistance training. Surface electromyography (SEMG) of proximal leg muscles was recorded during isometric knee extension at maximum voluntary contraction (MVC) and at 20, 40, 60 and 80% of MVC. Changes in MVC, maximum electrical activity and torque–EMG ratios (TER) were calculated. Fatigue was studied by determining the changes in endurance and in the decline of the median frequency (F_med) of the SEMG during a sustained contraction at 80% MVC. These parameters showed no significant changes after the training in either of the diagnostic groups. Only the Charcot-Marie-Tooth training group showed a gradual significant increase in mean MVC over the whole training period (21%). After 24 weeks, the increase in mean RMS was similar (25%), but this was mainly due to a sharp rise during the first 8 weeks of training (20%). The findings indicate that the initial strength increase was due to a neural factor, while the subsequent increase was mainly due to muscle hypertrophy.