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Alan K. Keenan Alma Gal Alexander Levitzki 《Biochemical and biophysical research communications》1982,105(2):615-623
Turkey erythrocyte adenylate cyclase was activated by GppNHp and l-epinephrine to its stable, highly active form. In this form the enzyme could be solubilized by Lubrol-PX and subsequently re-inserted into phospholipid vesicles concomitantly with the removal of up to 99.3% of the Lubrol. The ability of GTP and l-epinephrine to reverse the GppNHp/epinephrine activated state was taken as a measure for the reappearance of hormone sensitivity in the reconstituted vesicles. An incomplete but significant reappearance of hormone sensitivity in the reconstituted adenylate cyclase was achieved. This hormone sensitivity was found to be stereospecific for (?)epinephrine. The 125I-cyanopindolol binding properties of the reconstituted β-receptor depend on the nature of the detergent and the phospholipids used in the reconstitution. 相似文献
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Tim Salditt 《生物化学与生物物理学报:生物膜》2006,1758(9):1483-1498
The conformation and correlations of amphiphilic and antimicrobial peptides and the associated changes of lipid bilayers can be studied in oriented lipid membranes deposited on solid substrates. Here we review recent work on these systems, as studied by modern interface-sensitive X-ray and neutron scattering methods. Density profile, short range order of acyl chains and molecular conformations of peptides and lipids are probed in the fluid state of the bilayer. With an emphasis on technical aspects, we review recent work illustrating the potential of the methods and discuss its potential in the field. 相似文献
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Rui Ma Atanas Koulov Christopher Moulton Manju Basu Sipra Banerjee Holly Goodson Subhash Basu 《Glycoconjugate journal》2003,20(5):319-330
Apoptosis, or programmed cell death, plays an important role in many physiological and diseased conditions. Induction of apoptosis
in cancer cells has been monitored during the cells' progression to apoptosis by anti-cancer drugs and inhibitors of the cell
surface glycolipids, gangliosides and SA-Lex biosyntheses [Basu, S (1991) Glycobiology, 1, 469–475; and ibid, 427–435] in animal tissues and human carcinoma cells, respectively. Induction of apoptosis in cancer cells by cell surface
glycolipids in the human breast cancer (SKBR3) cells is the aim in this study. We have employed the disialosyl gangliosides
(GD3 and GD1b) to initiate apoptosis in SKBR3 cells grown in culture in the presence of 14C-L-Serine. At lower concentrations (0–20 μM) of exogenously added non-radioactive GD3, GD1b, or bovine ganglioside mixture
(GM1:GD1a:GD1b:GT1a 2:4:4:2), the incorporation of radioactivity in both 14C-sphingolipid and 14C-ceramide was higher. However, at higher concentrations (20–100 μM), wherein apoptosis occurred in high frequency, the 14C-incorporation decreased in both GSLs and ceramide. Apoptosis induction was monitored by the concomitant appearance of caspase-3
activation and the binding of a fluorescent dye PSS-380 to the outer leaflet of phosphatidyl-serine. These results indicated
that, in addition to many unknown cell surface glycoconjugates GD3 or GD1b (disialosyl ganglioside) could play an important
role in the regulation of breast carcinoma cell death. Published in 2004.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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